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Assessment of damage potential of Gnomoniopsis castaneae to fruit and trees of European Chestnut (Castanea sativa)

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  • Francesca CARLONI,
  • Carlo BREGANT,
  • Benedetto T. LINALDEDDU,
  • Giorgio MARESI,
  • Sergio MUROLO
Francesca CARLONI
Department of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche 10, 60131, Ancona, Italy
Carlo BREGANT
Department of Land and Agro-Forestry Systems, University of Padua, Viale dell’Università, 16, 35020 Legnaro, Italy
Benedetto T. LINALDEDDU
Department of Land and Agro-Forestry Systems, University of Padua, Viale dell’Università, 16, 35020 Legnaro, Italy
Giorgio MARESI
Fondazione Edmund Mach, Technology Transfer Center, Via E. Mach 1, 38010 San Michele all’Adige, Trento, Italy
Sergio MUROLO
Department of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche 10, 60131, Ancona, Italy
DOI: https://doi.org/10.36253/phyto-16789
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Published 2026-03-16

Keywords

  • endophytic and pathogenic behavior,
  • molecular typing,
  • pathogenicity test,
  • water stress

How to Cite

[1]
F. CARLONI, C. BREGANT, B. T. LINALDEDDU, G. MARESI, and S. MUROLO, “Assessment of damage potential of Gnomoniopsis castaneae to fruit and trees of European Chestnut (Castanea sativa)”, Phytopathol. Mediterr., Mar. 2026.

Copyright (c) 2026 Francesca CARLONI, Carlo BREGANT, Benedetto T. LINALDEDDU, Giorgio MARESI, Sergio MUROLO

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Gnomoniopsis castaneae has become an important pathogen of European chestnut (Castanea sativa), affecting fruits and colonizing shoots and branches, as well as galls caused by the oriental chestnut gall wasp (Dryocosmus kuriphilus). On apparently asymptomatic fruits, G. castaneae can colonize the endosperms, causing quality issues, especially in post-harvest. Given the alarming spread of G. castaneae infections in Italy and the current knowledge gaps regarding aspects of G. castaneae biogeography and virulence, research was addressed to: 1) evaluate occurrence of G. castaneae in chestnut nuts and branches in different Italian regions; 2) study occurrence and distribution of the two known G. castaneae haplotypes throughout Italy; and 3) evaluate their virulence on chestnut under different water regimes. Fungal isolation from representative chestnut branch and nut samples consistently yielded colonies that were morphologically consistent with G. castaneae, the identity of which was confirmed by analysis of ITS sequences. Analysis of β-tubulin sequences confirmed the presence of two distinct genetic lineages (Gc-haplotypes A and B). To assess pathogenicity, G. castaneae isolates were inoculated onto chestnuts, chestnut cuttings and 3-year-old young plants grown under two water regimes. All assessed isolates were pathogenic on chestnut, and water-stressed plants exhibited more extensive necrosis than well-watered plants when inoculated with the Gc-haplotype A, highlighting the influence of environmental conditions on disease expression. This study expands current knowledge on the distribution, genetic diversity, and effects of water stress on the pathogenic potential of G. castaneae on chestnut.

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References

  1. Bassanelli C., Bischetti G.B., Chiaradia E.A., Rossi L., Vergani, C., 2013. The contribution of chestnut coppice forests on slope stability in abandoned territory: a case study. Journal of Agricultural Engineering 44: (s2). https://doi.org/10.4081/jae.2013.254
  2. Battisti A., Bevegnu I., Colombari F., Haack R.A., 2014. Invasion by the chestnut gall wasp in Italy causes significant yield loss in Castanea sativa nut production. Agricultural and Forest Entomology 16: 75–79. https://doi.org/10.1111/afe.12036
  3. Bianco L., Fontana P., Marchesini A., Torre S., Moser M., … Palmieri L., 2024. The de novo, chromosome-level genome assembly of the sweet chestnut (Castanea sativa Mill.) Cv. Marrone Di Chiusa Pesio. BMC Genomic Data 25(1): 64. https://doi.org/10.1186/s12863-024-01245-7
  4. Bernard A., Hamdy S., Le Corre L., Dirlewanger E., Lheureux, F., 2020. 3D characterization of walnut morphological traits using X-ray computed tomography. Plant Methods 16(1): 115. https://doi.org/10.1186/s13007-020-00657-7
  5. Conedera M., Krebs P., Tinner W., Pradella M., … Torriani D., 2004. The cultivation of Castanea sativa (Mill.) in Europe, from its origin to its diffusion on a continental scale. Vegetation History and Archaeobotany 13(3): 161–179. https://doi.org/10.1007/s00334-004-0038-7
  6. Dar M.A., Rai M., 2015. Gnomoniopsis smithogilvyi, a canker causing pathogen on Castanea sativa: First report. Mycosphere 6(3): 327–336. https://doi.org/10.5943/mycosphere/6/3/8
  7. Dennert F.G., Broggini G.A.L., Gessler C., Storari M., 2015. Gnomoniopsis castaneae is the main agent of chestnut nut rot in Switzerland. Phytopathologia Mediterranea 54: 199–211. https://doi.org/10.14601/Phytopathol_Mediterr-14712
  8. Dobry E., Campbell M., 2023. Gnomoniopsis castaneae: an emerging plant pathogen and global threat to chestnut systems. Plant Pathology 72: 218–231. https://doi.org/10.1111/ppa.13670
  9. Doyle J.J., Doyle J.L., 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
  10. Donis González I.R., Fulbright D.W., Ryser E.T., Guyer D., 2009. Shell mold and kernel decay of fresh chestnuts in Michigan. In I European Congress on Chestnut-Castanea 2009 866: 353-362. https://doi.org/10.17660/ActaHortic.2010.866.45
  11. European and Mediterranean Plant Protection Organization, 2017. EPPO Reporting Service No. 02–2017 Num. Article: 2017/047. Available online: http://archives.eppo.int/EPPOReporting/2017/Rse-1702.pdf (accessed 22 March 2021).
  12. Freitas T.R., Santos J.A., Silva A.P., Fraga H., 2021. Influence of climate change on chestnut trees: a review. Plants 10: 1463. https://doi.org/10.3390/plants10071463
  13. Gabrielli A., 1994. La civiltà del castagno. Monti e boschi 65: 3.
  14. Gaffuri F., Longa C.M.O., Turchetti T., Danti R., Maresi G., 2017. ‘Pink rot’: infection of Castanea sativa fruits by Colletotrichum acutatum. Forest Pathology 47: e12307. https://doi.org/10.1111/efp.12307
  15. Glass N.L., Donaldson G.C., 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61: 1323–1330. https://doi.org/10.1128/AEM.61.4.1323-1330.1995
  16. González I.R., Guyer D.E., Fulbright D.W., 2016. Quantification and identification of microorganisms found on shell and kernel of fresh edible chestnuts in Michigan. Journal of the Science of Food and Agriculture 96: 4514–4522. https://doi.org/10.1002/jsfa.7667
  17. Kolp M., Double M. L., Fulbright D. W., MacDonald W. L., Jarosz A. M., 2020. Spatial and temporal dynamics of the fungal community of chestnut blight cankers on American chestnut (Castanea dentata) in Michigan and Wisconsin. Fungal Ecology 45:100925. https://doi.org/10.1016/j.funeco.2020.100925
  18. Lema F., Baptista P., Oliveira C., Ramalhosa E., 2023. Brown rot caused by Gnomoniopsis smithogilvyi (syn. Gnomoniopsis castaneae) at the level of the chestnut tree (Castanea sativa Mill.). Applied Sciences 13: 3969. https://doi.org/10.3390/app13063969
  19. Linaldeddu B.T., Deidda A., Scanu B., Franceschini A., Alves A., … Phillips A.J.L., 2016. Phylogeny, morphology and pathogenicity of Botryosphaeriaceae, Diatrypaceae and Gnomoniaceae associated with branch diseases of hazelnut in Sardinia (Italy). European Journal of Plant Pathology 146(2): 259–279. https://doi.org/10.1007/s10658-016-0912-z
  20. Lione G., Giordano L., Sillo, F., Gonthier P., 2015. Testing and modelling the effects of climate on the incidence of the emergent nut rot agent of chestnut Gnomoniopsis castanea. Plant Pathology 64: 852–863. https://doi.org/10.1111/ppa.12319
  21. Lione G.G., 2016. Ecology and epidemiology of the emerging plant pathogen Gnomoniopsis castaneae. Journal of Plant Pathology 98(4): S5-S5.
  22. Lione G., Danti R., Fernandez-Conradi P., Ferreira-Cardoso J.V., Lefort F., … Gonthier P., 2019. The emerging pathogen of chestnut Gnomoniopsis castaneae: the challenge posed by a versatile fungus. European Journal of Plant Pathology 153: 671–685. https://doi.org/10.1007/s10658-018-1597-2
  23. Lione G., Giordano L., Sillo F., Brescia F., Gonthier P., 2021. Temporal and spatial propagule deposition patterns of the emerging fungal pathogen of chestnut Gnomoniopsis castaneae in orchards of north‐western Italy. Plant Pathology 70(9): 2016–2033. https://doi.org/10.1111/ppa.13451
  24. Marais A., Murolo S., Faure C., Brans Y., Larue C., … Candresse, T., 2021. Sixty years from the first disease description, a novel badnavirus associated with chestnut mosaic disease. Phytopathology 111(6): 1051–1058. https://doi.org/10.1094/PHYTO-09-20-0420-R
  25. Maresi G., Oliveira Longa C.M., Turchetti T., 2013. Brown rot on nuts of Castanea sativa Mill: an emerging disease and its causal agent. iForest 6: 294–301. https://doi.org/10.3832/ifor0952-006
  26. Mattioli W., Mancini L. D., Portoghesi L., Corona P., 2016. Biodiversity conservation and forest management: The case of the sweet chestnut coppice stands in Central Italy. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology 150(3): 592-600. https://doi.org/10.1080/11263504.2015.1054448
  27. Matsui H., 2022. Biological Sensing Using Infrared. Biosignal Processing 209.
  28. McKinney H.H., 1923. Influence of soil temperature and moisture on infection of wheat seedlings by Helminthosporium sativum. Journal of Agricultural Research 26: 195–218.
  29. Morales-Rodriguez C., Bastianelli G., Caccia R., Bedini G., Massantini R., … Vannini A., 2022. Impact of ‘brown rot’ caused by Gnomoniopsis castaneae on chestnut fruits during the post-harvest process: critical phases and proposed solutions. Journal of the Science of Food and Agriculture 102: 680–687. https://doi.org/10.1002/jsfa.11397
  30. Nicoletti R., Beccaro G.L., Sekara A., Cirillo C., Di Vaio C., 2021. Endophytic fungi and ecological fitness of chestnuts. Plants 10: 542. https://doi.org/10.3390/plants10030542
  31. Pasche S., Calmin G., Auderset G., Crovadore J., Pelleteret P., … Lefort F., 2016. Gnomoniopsis smithogilvyi causes chestnut canker symptoms in Castanea sativa shoots in Switzerland. Fungal Genetics and Biology 87: 9–21. https://doi.org/10.1016/j.fgb.2016.01.002
  32. Pérez-Girón J.C., Álvarez-Álvarez P., Díaz-Varela E.R., Mendes Lopes D.M., 2020. Influence of climate variations on primary production indicators and on the resilience of forest ecosystems in a future scenario of climate change: application to sweet chestnut agroforestry systems in the Iberian Peninsula. Ecological Indicators 113: 106199. https://doi.org/10.1016/j.ecolind.2020.106199
  33. Pezzi G., Ferretti F., Maltoni A., Krebs P., Conedera M., Maresi G., 2022. The chestnut orchards in the Bolognese Apennines: A vanishing socio-ecological habitat. In Historical Ecology: Learning from the Past to Understand the Present and Forecast the Future of Ecosystems pp. 195–205.
  34. Possamai G., Dallemole-Giaretta R., Gomes-Laranjo J., Sampaio A., Rodrigues P., 2023. Chestnut brown rot and Gnomoniopsis smithogilvyi: Characterization of the causal agent in Portugal. Journal of Fungi 9(4): 401. https://doi.org/10.3390/jof9040401
  35. Prada M., Bravo F., Berdasco L., Canga E., Martínez-Alonso C., 2016. Carbon sequestration for different management alternatives in sweet chestnut coppice in northern Spain. Journal of Cleaner Production 135: 1161-1169. https://doi.org/10.1016/j.jclepro.2016.07.041
  36. Prospero S., Heinz M., Augustiny E., Chen Y. Y., Engelbrecht J., … Fonti P., 2023. Distribution, causal agents, and infection dynamic of emerging ink disease of sweet chestnut in Southern Switzerland. Environmental Microbiology 25(11): 2250–2265. https://doi.org/10.1111/1462-2920.16455
  37. Rigling D., Prospero S., 2018. Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control. Molecular Plant Pathology 19(1): 7–20. https://doi.org/10.1111/mpp.12542
  38. Rodrigues P., Driss O.J., Gomes-Laranjo J., Sampaio A., 2022. Impact of cultivar, processing and storage on the mycobiota of European chestnut fruits. Agriculture 12: 1930. https://doi.org/10.3390/agriculture12111930
  39. Santos M.J., Pinto T., Vilela A., 2022. Sweet chestnut (Castanea sativa Mill.) nutritional and phenolic composition interactions with chestnut flavor physiology. Foods 11: 4052. https://doi.org/10.3390/foods11244052
  40. Schulz B., Guske S., Dammann U., Boyle C., 1998. Endophyte host interactions II. Defining symbiosis of the endophyte-host interaction. Symbiosis 25: 213–227.
  41. Seddaiu S., Mello A., Sechi C., Cerboneschi A., Linaldeddu B.T., 2021. First Report of Neofusicoccum parvum associated with chestnut nut rot in Italy. Plant Disease 105: 3743. https://doi.org/10.1094/PDIS-01-21-0072-PDN
  42. Seddaiu S., Mello A., Sarais L., Mulas A., Sechi C., Ruiu P.A., … Linaldeddu B.T., 2023. Haplotypes distribution and virulence of Gnomoniopsis castaneae in Italy. Journal of Plant Pathology 105: 1135–1140. https://doi.org/10.1007/s42161-023-01459-1
  43. Shuttleworth L.A., 2012 . Fungal planet description sheet 108: Gnomoniopsis smithogilvyi LA Shuttleworth, ECY Liew & DI Guest, sp. nov. Persoonia 28: 142–143. https://doi.org/10.3767/003158512X652633
  44. Shuttleworth L.A., Liew E.C.Y., Guest D.I., 2013. Survey of the incidence of chestnut rot in south-eastern Australia. Australasian Plant Pathology 42: 63–72. https://doi.org/10.1007/s13313-012-0170-2
  45. Shuttleworth L.A., Guest D.I., 2017. The infection process of chestnut rot, an important disease caused by Gnomoniopsis smithogilvyi (Gnomoniaceae, Diaporthales) in Oceania and Europe. Australasian Plant Pathology 46(5): 397–405. https://doi.org/10.1007/s13313-017-0502-3
  46. Suna S., Avşar B., Koçer S., Çopur Ö.U., 2021. Effects of different pretreatments on the physicochemical characteristics and quality criteria of chestnut (Castanea sativa Mill.) pickle: a new value-added product. Journal of Food Processing and Preservation 45:e15669. https://doi.org/10.1111/jfpp.15669
  47. Topalidou E., Lagiotis G., Bosmali I., Stefanidou E., Tsirogiannis D., Vettraino A.M., Madesis P., 2024. Water stress enhances the pathogenicity of Gnomoniopsis castaneae in chestnut trees. Journal of Plant Pathology 106: 345–352. https://doi.org/10.1016/j.funbio.2024.06.003
  48. Ugolini F., Massetti L., Pedrazzoli F., Tognetti R., Vecchione A., … Maresi G., 2014. Ecophysiological responses and vulnerability to other pathologies in European chestnut coppices, heavily infested by the Asian chestnut gall wasp. Forest Ecology and Management 314: 38–49. https://doi: 10.1016/j.foreco.2013.11.031
  49. Vettraino A. M., Luchi N., Rizzo D., Pepori A. L., Pecori F., Santini A., 2021. Rapid diagnostics for Gnomoniopsis smithogilvyi (syn. Gnomoniopsis castaneae) in chestnut nuts: new challenges by using LAMP and real-time PCR methods. AMB Express 11(1): 105. https://doi.org/10.1186/s13568-021-01266-w
  50. Villa S., Marchesini A., Torre S., Bianco L., Fontana P., De Quattro C., … Sebastiani F., 2025. First complete mitogenome assembly of Castanea sativa: structure, comparative genomics, and phylogeny. Tree Genetics & Genomes 21(4): 22. https://doi.org/10.1007/s11295-025-01707-8
  51. Visentin I., Gentile S., Valentino D., Gonthier P., Tamietti G., Cardinale F., 2012. Gnomoniopsis castaneae sp. nov. (Gnomoniaceae, Diaporthales) as the causal agent of nut rot in sweet chestnut. Journal of Plant Pathology 94: 411–419. http://www.jstor.org/stable/45156050.
  52. Washington W.S., Allen A.D., Dooley L.B., 1997. Preliminary studies on Phomopsis castanea and other organisms associated with healthy and rotted chestnut fruit in storage. Australasian Plant Pathology 26: 37–43. https://doi.org/10.1071/AP97006
  53. White T.J., Bruns T., Lee S., Taylor J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. (Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. ed.). San Diego, C, USA, Academic Press, 315–322.

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