First report of camellia ring spot associated virus 3 in Camellia japonica in Europe
Francesco DESIDERIO- Myeonghwan KWAK
- Eui-Joon KIL
- Giuseppe PARRELLA
Published 2026-07-12
Keywords
- CRSaV-3,
- High Throughput Sequencing,
- leaf variegation,
- chlorotic spots,
- virus detection
How to Cite
Copyright (c) 2026 Francesco DESIDERIO, Myeonghwan KWAK, Eui-Joon KIL, Giuseppe PARRELLA
This work is licensed under a Creative Commons Attribution 4.0 International License.
Funding data
-
Consiglio Nazionale delle Ricerche
Grant numbers Short Term Mobility STM 2024
Abstract
Numerous viruses have been described in camellia, often found in association, and a variety of symptoms have been associated with these infections. In this study, a plant with chlorotic spots, including complete yellowing of the leaves, was subjected to high-throughput sequencing (HTS). Three viral contigs were identified by HTS, and complete genome sequences of a new isolate of camellia ring spot associated virus 3 (CRSaV-3) were determined using nested PCR to bridge the gap between discontinuous contigs. The new CRSaV-3 isolate shared the highest percentage of nucleotide identity (87.1%) with the American isolate CJ5_676. This represents the first report of CRSaV-3 in symptomatic camellia in Europe and, since only this virus was diagnosed, for the first time it was possible to reliably associate the symptoms observed in camellia to a specific CRSaV-3 isolate.
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References
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