Vol. 63 No. 1 (2024)
Articles

Mixed infections of Tomato yellow leaf curl New Delhi virus and a ‘Candidatus Phytoplasma asteris’ strain in zucchini squash in Italy

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  • Giuseppe PARRELLA,
  • Elisa TROIANO
Giuseppe PARRELLA
Institute for Sustainable plant Protection of National Research Council (IPSP-CNR), Piazzale Enrico Fermi 1, 80055 Portici (NA)
Elisa TROIANO
Institute for Sustainable plant Protection of National Research Council (IPSP-CNR), Piazzale Enrico Fermi 1, 80055 Portici (NA)
DOI: https://doi.org/10.36253/phyto-15110
Categories

Published 2024-04-30

Keywords

  • Aster yellow phytoplasma,
  • ToLCNDV,
  • Cucurbita pepo,
  • mixed infection

How to Cite

[1]
G. PARRELLA and E. TROIANO, “Mixed infections of Tomato yellow leaf curl New Delhi virus and a ‘Candidatus Phytoplasma asteris’ strain in zucchini squash in Italy”, Phytopathol. Mediterr., vol. 63, no. 1, pp. 73–78, Apr. 2024.

Copyright (c) 2024 Giuseppe PARRELLA, Elisa TROIANO

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

A new disease syndrome of zucchini squash was observed in Southern Italy, in 2018 and again in 2020. Affected plants were severely stunted and leaves were bent downwards, small, stiff, thick, leathery, and had interveinal chloroses. In addition, flowers were virescent and fruits were deformed and often cracked. Disease incidence was 20 and 30% in two different zucchini cultivations in Campania region (Southern Italy). Tomato yellow leaf curl New Delhi virus (ToLCNDV) was detected in eight samples, by loop-mediated isothermal amplification–based (LAMP) kit and by PCR and Sanger sequencing of the AV1 gene. Phytoplasmas were detected in the same samples using nested PCR assays with primer pairs P1/P7 and R16F2n/R16R2. Phytoplasma associations in plant samples were confirmed using specific primers for the multilocus genes SecY, tuf and rp. Sequence comparison of multilocus genes and phylogenetic analyses of the 16S rDNA gene confirmed the association of a phytoplasma strain closely related to ‘Candidiatus Phytoplamsa asteris’. This is the first report of mixed infections of ToLCNDV and a putative ‘Ca. Phytoplamsa asteris’ strain in zucchini, associated with a new Squash-Phytoplasma-Begomovirus (SqPB) disease syndrome.

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