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Occurrence and genetic diversity of grapevine leafroll-associated virus 4 in Algeria

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  • Anfel DJENAOUI,
  • Osamah ALISAWI,
  • Manel EL AIR,
  • Nour Elhouda LAIDOUDI,
  • Imene MAHDID,
  • Rima Hind BOUDCHICHA,
  • Naima MAHFOUDHI,
  • Arezki LEHAD
Anfel DJENAOUI
Laboratoire de Phytopathologie et Biologie Moléculaire, Département de Botanique, Ecole Nationale Supérieure Agronomique (ENSA-ES 1603), Avenue Hassan Badi, El-Harrach, Algiers, 16200, Algeria
Osamah ALISAWI
Department of Plant Protection, Faculty of Agriculture, University of Kufa, Najaf, Iraq
Manel EL AIR
Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 1004 El Menzah, Tunis, Tunisia
Nour Elhouda LAIDOUDI
Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université Ferhat Abbas Sétif 1, Setif, Algeria
Imene MAHDID
Laboratoire de Phytopathologie et Biologie Moléculaire, Département de Botanique, Ecole Nationale Supérieure Agronomique (ENSA-ES 1603), Avenue Hassan Badi, El-Harrach, Algiers, 16200, Algeria
Rima Hind BOUDCHICHA
Biotechnology Research Center, Constantine (CRBT), Constantine, Algeria
Naima MAHFOUDHI
Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 1004 El Menzah, Tunis, Tunisia
Arezki LEHAD
Laboratoire de Phytopathologie et Biologie Moléculaire, Département de Botanique, Ecole Nationale Supérieure Agronomique (ENSA-ES 1603), Avenue Hassan Badi, El-Harrach, Algiers, 16200, Algeria
DOI: https://doi.org/10.36253/phyto-16539
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Published 2026-03-16

Keywords

  • GLRaV-4,
  • RT-PCR,
  • P23,
  • genetic diversity,
  • high throughput sequencing

How to Cite

[1]
A. DJENAOUI, “Occurrence and genetic diversity of grapevine leafroll-associated virus 4 in Algeria”, Phytopathol. Mediterr., Mar. 2026.

Copyright (c) 2026 Anfel DJENAOUI, Osamah ALISAWI, Manel EL AIR, Nour Elhouda LAIDOUDI, Imene MAHDID, Rima Hind BOUDCHICHA, Naima MAHFOUDHI, Arezki LEHAD

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Grapevine leafroll-associated virus 4 (GLRaV-4, Ampelovirus tetravitis) has considerable genetic diversity. A survey was conducted in central, western, and southern Algeria to investigate the distribution and the genetic diversity of GLRaV-4 in commercial and autochthonous grape cultivars. DAS-ELISA detected an overall grapevine infection rate of 18.2%, and infections in a subset of samples were confirmed by RT-PCR. Analysis of the P23 protein gene sequence revealed six known phylogenetic groups, with Algerian isolates clustering in strains -4, -5, -6, -9, and -Pr of GLRaV-4. In addition to this virus, three viruses and two viroids were identified using high throughput sequencing: grapevine Pinot gris virus (GPGV, Trichovirus pinovitis), grapevine leafroll-associated virus 2 (GLRaV-2, Closterovirus vitis), grapevine fanleaf virus (GFLV, Nepovirus foliumflabelli), hop stunt viroid (HSVd, Hostuviroid impedihumuli) and grapevine yellow speckle viroid 1 (GYSVd-1, Apscaviroid alphaflavivitis). This study was the first research on genetic diversity of GLRaV-4 in Algeria.

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