Vol. 57 No. 2 (2018)
Research Papers

Prevalence and genetic diversity of Grapevine virus A in Tunisia

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  • Ilhem SELMI,
  • Arezki LEHAD,
  • Davide PACIFICO,
  • Francesco CARIMI,
  • Naima Mahfoudhi
Ilhem SELMI
Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 1004 ElMenzah, Tunis, Tunisie
Arezki LEHAD
Laboratoire de Phytopathologie et Biologie Moléculaire. Ecole Nationale Supérieure d’Agronomie, Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, Algérie
Davide PACIFICO
Istituto di Bioscienze e BioRisorse (IBBR), Consiglio Nazionale delle Ricerche- CNR Corso Calatafimi 414, 90129 Palermo, Italia
Francesco CARIMI
Istituto di Bioscienze e BioRisorse (IBBR), Consiglio Nazionale delle Ricerche- CNR Corso Calatafimi 414, 90129 Palermo, Italia
Naima Mahfoudhi
Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 1004 ElMenzah, Tunis, Tunisie
DOI: https://doi.org/10.14601/Phytopathol_Mediterr-22123

Published 2018-09-17

Keywords

  • grapevine,
  • GVA,
  • molecular analysis,
  • diversity

How to Cite

[1]
I. SELMI, A. LEHAD, D. PACIFICO, F. CARIMI, and N. Mahfoudhi, “Prevalence and genetic diversity of Grapevine virus A in Tunisia”, Phytopathol. Mediterr., vol. 57, no. 2, pp. 237–244, Sep. 2018.

Copyright (c) 2018 Ilhem SELMI, Arezki LEHAD, Davide PACIFICO, Francesco CARIMI, Naima Mahfoudhi

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Prevalence and genetic diversity of the complete CP gene of Grapevine virus A (GVA) were assessed in isolates from rootstocks, wine and table grape varieties and autochthonous grapevines. Wine grapes were the most infected (63%), followed by table grapes (49%) and rootstocks (44%). Autochthonous grapevines were the less infected (35%). Analyses of the complete coat protein sequences of 20 GVA isolates from the main grapevine growing areas of Tunisia identified three phylogroups, accounting, respectively, for 70% (group I), 25% (group IV) and 5% (group III) of the isolates. No sequences clustered into group II. Phylogenetic analyses indicated that Tunisian GVA isolates are not grouped by the host cultivar or geographic origin.

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