Vol. 63 No. 3 (2024)
Articles

Genetic diversity of GRSPaV-associated virus in Algeria

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  • Adel BACHIR,
  • Manel EL AIR,
  • Osamah ALISAWI,
  • Anfel DJENAOUI,
  • Nourelhouda LAIDOUDI,
  • Imane MAHDID,
  • Bilal YAHIAOUI,
  • Meriem LOUANCHI,
  • Naima MAHFOUDHI,
  • Arezki LEHAD
Adel BACHIR
Laboratoire de Phytopathologie et Biologie Moléculaire, Ecole Nationale Supérieure d’Agronomie, Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, Algeria
Manel EL AIR
Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 1004 El Menzah, Tunis, Tunisia
Osamah ALISAWI
Department of Plant Protection, Faculty of Agriculture, University of Kufa, Najaf, Iraq
Nourelhouda LAIDOUDI
Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université Ferhat Abbas Sétif1, Algérie
Imane MAHDID
Laboratoire de Phytopathologie et Biologie Moléculaire, Ecole Nationale Supérieure d’Agronomie, Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, Algeria
Bilal YAHIAOUI
Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université Ferhat Abbas Sétif1, Algérie
Meriem LOUANCHI
Laboratoire de Phytopathologie et Biologie Moléculaire, Ecole Nationale Supérieure d’Agronomie, Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, Algeria
Naima MAHFOUDHI
Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 1004 El Menzah, Tunis, Tunisia
Arezki LEHAD
Laboratoire de Phytopathologie et Biologie Moléculaire, Ecole Nationale Supérieure d’Agronomie, Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, Algeria
DOI: https://doi.org/10.36253/phyto-15554
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Published 2024-12-30

Keywords

  • GRSPav,
  • high throughput sequencing,
  • RT-PCR

How to Cite

[1]
A. BACHIR, “Genetic diversity of GRSPaV-associated virus in Algeria”, Phytopathol. Mediterr., vol. 63, no. 3, pp. 443–451, Dec. 2024.

Copyright (c) 2024 Adel BACHIR, Manel EL AIR, Osamah ALISAWI, Anfel DJENAOUI, Nourelhouda LAIDOUDI, Imane MAHDID, Bilal YAHIAOUI, Meriem LOUANCHI, Naima MAHFOUDHI, Arezki LEHAD

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Grapevine rupestris stem pitting-associated virus (GRSPaV; Foveavirus rupestris, Betaflexiviridae) is the most widespread grapevine virus in most viticulture regions, and this virus has high genetic variability. Genetic diversity of GRSPaV in Algeria was examined, based on the complete capsid protein (CP) gene. Similarity proportions between the CP sequences varied from 76% to 99%. The complete coding sequence of Algerian GRSPaV isolate ALG99 (8,646 nt) was determined using high-throughput sequencing and bioinformatic analyses. Algerian GRSPaV isolates clustered into four groups, with most sequences in groups III and IV. This research is the first to determine the genetic variability of GRSPaV in Algeria.

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