Vol. 64 No. 2 (2025)
Articles

Occurrence of Grapevine fanleaf virus in Algerian vineyards, and complete genome sequencing

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  • Nour Elhouda LAIDOUDI,
  • Osamah ALISAWI,
  • Bilal YAHIAOUI,
  • Anfel DJENAOUI,
  • Imane MAHDID,
  • Adel BACHIR,
  • Francesca DE LUCA,
  • Elena FANELLI,
  • Angelantonio MINAFRA,
  • Naima MAHFOUDHI,
  • Arezki LEHAD
Nour Elhouda LAIDOUDI
Laboratory of applied Microbiology, Department of Agronomy, Faculty of Natural and Life Sciences, Ferhat Abbas University
Osamah ALISAWI
Department of Plant Protection, Faculty of Agriculture, University of Kufa, Najaf, Iraq
Bilal YAHIAOUI
Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, Ferhat Abbas University, Setif, Algeria
Anfel DJENAOUI
Phytopathology and molecular biology laboratory, National high school of agronomy, Belfort, Elharrach, Algiers, Algeria
Imane MAHDID
Phytopathology and molecular biology laboratory, National high school of agronomy, Belfort, Elharrach, Algiers, Algeria
Adel BACHIR
Phytopathology and molecular biology laboratory, National high school of agronomy, Belfort, Elharrach, Algiers, Algeria
Francesca DE LUCA
Istituto per la Protezione delle Piante, UOS-Bari, Consiglio Nazionale delle Richerche (C.N.R.), Via Amendola 122/D, 70126 Bari, Italy
Elena FANELLI
Istituto per la Protezione delle Piante, UOS-Bari, Consiglio Nazionale delle Richerche (C.N.R.), Via Amendola 122/D, 70126 Bari, Italy
Angelantonio MINAFRA
Istituto per la Protezione delle Piante, UOS-Bari, Consiglio Nazionale delle Richerche (C.N.R.), Via Amendola 122/D, 70126 Bari, Italy
Naima MAHFOUDHI
Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 2049 Ariana, Tunisia
Arezki LEHAD
Phytopathology and molecular biology laboratory, National high school of agronomy, Belfort, Elharrach, Algiers, Algeria
DOI: https://doi.org/10.36253/phyto-15993

Published 2025-09-12

Keywords

  • DAS-ELISA,
  • High Throughput Sequencing,
  • RT-PCR,
  • Grapevine,
  • GFLV

How to Cite

[1]
N. E. LAIDOUDI, “Occurrence of Grapevine fanleaf virus in Algerian vineyards, and complete genome sequencing”, Phytopathol. Mediterr., vol. 64, no. 2, pp. 219–228, Sep. 2025.

Copyright (c) 2025 Nour Elhouda LAIDOUDI, Osamah ALISAWI, Bilal YAHIAOUI, Anfel DJENAOUI, Imane MAHDID, Adel BACHIR, Francesca DE LUCA, Elena FANELLI, Angelantonio MINAFRA, Naima MAHFOUDHI, Arezki LEHAD

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Prevalence and genetic diversity of Grapevine fanleaf virus (Nepovirus foliumflabelli, GFLV) were determined in vineyards and grape varieties in Algeria. Samples (414) from different cultivars and viticulture areas were screened using DAS-ELISA and partially confirmed by RT-PCR, revealing 21% infection incidence. In Ahmer Bou Amer the greatest incidence of infection was recorded (61%). Some vines, confirmed to be GFLV-infected, had characteristic symptoms of leaf yellowing, chloroses, and mosaic patterns, reducing vine vigour and fruit quality. High throughput sequencing and bioinformatics analyses of a single GFLV-infected accession obtained a nearly complete grapevine fanleaf virus RNA1 consensus sequence of 5,979 nt, and an RNA2 with complete consensus sequence of 3,711 nt. Grapevine yellow speckle viroid, Hop stunt viroid and other viruses were also identified in the ‘background’ virome. Phylogenetic analyses of an amplified fragment of the GFLV coat protein gene from some of the accessions indicated close genetic relationships between Algerian and Russian/United States of America GFLV isolates, suggesting potential shared origins or transmission pathways. These results emphasize the need for implementing strict phytosanitary measures (e.g. use of virus-free planting material) to mitigate GFLV spread and its detrimental effects on grapevine production in Algeria.

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