Vol. 54 No. 3 (2015)
Research Papers

On-site detection of Xylella fastidiosa in host plants and in “spy insects” using the real-time loop-mediated isothermal amplification method

Sandro DRAGO
Bionat Italia s.r.l. via Aquileia, 34, 90144 Palermo, Italy
Franco VALENTINI
CIHEAM - Istituto Agronomico Mediterraneo, Via Ceglie 9, 70010 Valenzano (BA), Italy
Toufic ELBEAINO
CIHEAM - Istituto Agronomico Mediterraneo, Via Ceglie 9, 70010 Valenzano (BA), Italy
Giuseppe STAMPONE
Bionat Italia s.r.l. via Aquileia, 34, 90144 Palermo, Italy
Anna D'ONGHIA
CIHEAM - Istituto Agronomico Mediterraneo, Via Ceglie 9, 70010 Valenzano (BA), Italy

Published 2015-09-24

Keywords

  • real-time LAMP,
  • Philaenus spumarius

How to Cite

[1]
T. YASEEN, “On-site detection of Xylella fastidiosa in host plants and in ‘spy insects’ using the real-time loop-mediated isothermal amplification method”, Phytopathol. Mediterr., vol. 54, no. 3, pp. 488–496, Sep. 2015.

Abstract

A recent severe outbreak of Xylella fastidiosa associated with ‘olive quick decline syndrome’ (OQDS) was reported in Apulia (Southern Italy). In this study an on-site real-time loop-mediated isothermal amplification (real-time LAMP) was developed for detecting X. fastidiosa in host plants and insects. A marked simplification of the DNA extraction procedure was obtained by heating the samples in a portable Smart-Dart device and using an optimized enhancer reaction buffer. The connection to a tablet or Smartphone allowed to visualize the results of the reaction in real time. Compared to PCR and ELISA, with which it showed comparable results in terms of sensitivity and reliability in the X. fastidiosa detection, this simplified real-time LAMP procedure proved to be “user friendly”, displaying the advantages to be an on-site detection method of easy handling, rapid execution and low cost.

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