Vol. 51 No. 3 (2012)
Research Papers

Phytophthora nicotianae is the predominant Phytophthora species in citrus nurseries in Egypt

Yosra AHMED
CIHEAM/Mediterranean Agronomic Institute of Bari, Via Ceglie 9, 70010 Valenzano (BA), Italy
Anna Maria D’ONGHIA
CIHEAM/Mediterranean Agronomic Institute of Bari, Via Ceglie 9, 70010 Valenzano (BA), Italy
Antonio IPPOLITO
Dipartimento di Biologia e Chimica Agro-forestale ed Ambientale, Università degli Studi di Bari, Via Amendola 165/A, Bari, Italy
Hamed EL SHIMY
Plant Pathol. Res. Inst., Agric. Res. Centre, Giza, Egypt
Gabriella CIRVILLERI
Dipartimento di Scienze e Tecnologie Fitosanitarie, Università degli Studi, Via S. Sofia 100, 95123 Catania, Italy
Thaer YASEEN
CIHEAM/Mediterranean Agronomic Institute of Bari, Via Ceglie 9, 70010 Valenzano (BA), Italy

Published 2012-11-02

Keywords

  • Phytophthora root rot,
  • P. citrophthora,
  • P. palmivora,
  • seasonal variation

How to Cite

[1]
Y. AHMED, A. M. D’ONGHIA, A. IPPOLITO, H. EL SHIMY, G. CIRVILLERI, and T. YASEEN, “Phytophthora nicotianae is the predominant Phytophthora species in citrus nurseries in Egypt”, Phytopathol. Mediterr., vol. 51, no. 3, pp. 519–527, Nov. 2012.

Abstract

Phytophthora root rot is considered to be the most destructive disease to citrus production in Egypt. Phytophthora species are generally present in citrus nurseries, where soil pots containing the survival propagules are considered responsible for their spread into new orchards. The goal of this study was to investigate the distribution and seasonal variation of Phytophthora species in soil and feeder roots in two Egyptian citrus nurseries, characterized by different management, and to identify Phytophthora species associated with root rot. Soil and root samples were collected at monthly intervals from Sour orange and Volkameriana lemon rootstocks during March-July period. The inoculum density of Phytophthora species, and the percentage of infected feeder roots, were estimated using the plate dilution method in conjunction with selective media. Phytophthora isolates were identified according to their morphological characteristics and on the basis of the ITS regions of the rDNA. Phytophthora nicotianae was the predominant isolated species, followed by P. citrophthora and P. palmivora. Phytophthora nicotianae was detected in both nurseries, while P. citrophthora and P. palmivora were recovered only in one nursery. Inoculum density of Phytophthora species fluctuated during spring and summer according to the environmental conditions, rootstock, and nursery management practices.

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