Vol. 62 No. 3 (2023)
Articles

Identification of pathogens causing brown rot of stone fruit in Cuneo province (Italy) and assessment of sensitivity to azoxystrobin, cyprodinil, fenhexamid, fludioxonil, and tebuconazole

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  • Greta DARDANI,
  • Vladimiro GUARNACCIA,
  • Luca NARI,
  • Stefanos I. TESTEMPASIS,
  • George S. KARAOGLANIDIS,
  • M. Lodovica GULLINO
Greta DARDANI
Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Braccini 2, 10095 Grugliasco (TO)
Vladimiro GUARNACCIA
Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Braccini 2, 10095 Grugliasco (TO)
Luca NARI
AGRION, The Foundation for Research, Innovation and Technological Development of Piedmont Agriculture, 12030 Manta
Stefanos I. TESTEMPASIS
Laboratory of Plant Pathology, Aristotle University of Thessaloniki, P.O. Box 269, 54124, Thessaloniki
George S. KARAOGLANIDIS
Laboratory of Plant Pathology, Aristotle University of Thessaloniki, P.O. Box 269, 54124, Thessaloniki
M. Lodovica GULLINO
Interdipartimental Centre for Innovation in the Agro-Environmental Sector, AGROINNOVA, University of Torino, Largo Braccini 2, 10095 Grugliasco (TO)
DOI: https://doi.org/10.36253/phyto-14399
Categories

Published 2023-12-30

Keywords

  • Fungal characterization,
  • Chemical control,
  • Monilinia

How to Cite

[1]
G. DARDANI, V. GUARNACCIA, L. NARI, S. I. TESTEMPASIS, G. S. KARAOGLANIDIS, and M. L. GULLINO, “Identification of pathogens causing brown rot of stone fruit in Cuneo province (Italy) and assessment of sensitivity to azoxystrobin, cyprodinil, fenhexamid, fludioxonil, and tebuconazole”, Phytopathol. Mediterr., vol. 62, no. 3, pp. 455–465, Dec. 2023.

Copyright (c) 2023 Greta DARDANI, Vladimiro GUARNACCIA, Luca NARI, Stefanos I. TESTEMPASIS, George S. KARAOGLANIDIS, M. Lodovica GULLINO

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Abstract

Monilinia spp. cause brown rot and blossom blight of stone fruit. This study characterized the diversity of Monilinia spp. associated with stone fruit rots in the Cuneo province, the major fruit production area in Piedmont, and assessed their sensitivity to azoxystrobin, cyprodinil, fenhexamid, fludioxonil and tebuconazole. Species diversity was determined by PCR amplification and sequencing of isolate internal transcribed spacer (ITS) regions. Sensitivity to fungicides was determined by measuring in vitro mycelium growth on fungicide-amended media. Fifty isolates were obtained from apricot, cherry, or peach fruits with typical brown rot symptoms. Thirteen isolates were identified as M. fructicola, and 37 as M. laxa. Nine isolates of Monilinia laxa and two of M. fructicola had resistance factor (RF) values greater than 10 for different fungicides. The greatest (RF) value (48.96) was measured for azoxystrobin against the M. fructicola isolate CVG 1514. Among the M. laxa isolates, isolate CVG 1547 had the greatest RF value to cyprodinil, while isolate CVG 1709 had RF values greater than 10 for cyprodinil and tebuconazole. A systematic and wider sampling should be carried out in the Piedmont region to determine the distribution of fungicide resistant Monilinia spp. in stone fruit crops. The use of site-specific fungicides remains the most effective strategy for control brown rot, and continued monitoring for fungicide resistance within Monilinia spp. populations is recommended.

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