Vol. 55 No. 3 (2016)
Research Papers

Simultaneous detection of mixed ‘Candidatus Phytoplasma asteris’ and ‘Ca. Liberibacter solanacearum’ infection in carrot

Eleonora SATTA
Alma Mater Studiorum - University of Bologna
Ana RAMIREZ
Universidad de Las Palmas de Gran Canaria
Samanta PALTRINIERI
Alma Mater Studiorum - University of Bologna
Nicoletta CONTALDO
Alma Mater Studiorum - University of Bologna
Purifacation BENITO
Consejería de Sector Primario y Soberanía Alimentaria del Cabildo de Gran Canaria
José POVEDA
Universidad de Las Palmas de Gran Canaria
Assunta BERTACCINI
Alma Mater Studiorum - University of Bologna

Published 2017-01-08

Keywords

  • ‘Candidatus Liberibacter solanacearum’,
  • ‘Candidatus Phytoplasma,
  • haplotypes,
  • nested PCR

How to Cite

[1]
E. SATTA, “Simultaneous detection of mixed ‘Candidatus Phytoplasma asteris’ and ‘Ca. Liberibacter solanacearum’ infection in carrot”, Phytopathol. Mediterr., vol. 55, no. 3, pp. 401–409, Jan. 2017.

Abstract

Symptoms of shoot and root malformation were observed in carrot plants in fields located in the North of Gran Canaria Island (Spain), during surveys carried out in spring 2015 and 2016. Total DNA extraction from the leaves of symptomatic plants was performed and PCR assays were carried out to detect the agents possibly associated to the observed symptoms. While ‘Candidatus Liberibacter solanacearum’ was detected in the majority of tested symptomatic samples, phytoplasmas belonging to the ribosomal group 16SrI were detected only in two samples. Phylogenetic analyses and sequencing, together with virtual RFLP, confirmed that ‘Ca. L. solanacearum’ strains detected in carrot samples belong to haplotype D.

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