Simultaneous detection of mixed ‘Candidatus Phytoplasma asteris’ and ‘Ca. Liberibacter solanacearum’ infection in carrot
Published 2017-01-08
Keywords
- ‘Candidatus Liberibacter solanacearum’,
- ‘Candidatus Phytoplasma,
- haplotypes,
- nested PCR
How to Cite
Copyright (c) 2017 Eleonora SATTA, Ana RAMIREZ, Samanta PALTRINIERI, Nicoletta CONTALDO, Purifacation BENITO, José POVEDA, Assunta BERTACCINI
This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
Symptoms of shoot and root malformation were observed in carrot plants in fields located in the North of Gran Canaria Island (Spain), during surveys carried out in spring 2015 and 2016. Total DNA extraction from the leaves of symptomatic plants was performed and PCR assays were carried out to detect the agents possibly associated to the observed symptoms. While ‘Candidatus Liberibacter solanacearum’ was detected in the majority of tested symptomatic samples, phytoplasmas belonging to the ribosomal group 16SrI were detected only in two samples. Phylogenetic analyses and sequencing, together with virtual RFLP, confirmed that ‘Ca. L. solanacearum’ strains detected in carrot samples belong to haplotype D.