Vol. 53 No. 2 (2014): Special Issue on FutureIPM
New or Unusual Disease Reports

Isolation and pathogenicity of Xylella fastidiosa from grapevine and almond in Iran

Naser AMANIFAR
Department of Plant Protection Research, Agricultural and Natural Resources Research Center of Chahar Mahal va Bakhtiari, Shahrekord, Iran
Mohsen TAGHAVI
Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran,
Karamat IZADPANAH
Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran,
Ghobad BABAEI
Department of Plant Protection Research, Agricultural and Natural Resources Research Center of Chahar Mahal va Bakhtiari, Shahrekord Iran

Published 2014-09-06

Keywords

  • leaf scorch

How to Cite

[1]
N. AMANIFAR, M. TAGHAVI, K. IZADPANAH, and G. BABAEI, “Isolation and pathogenicity of Xylella fastidiosa from grapevine and almond in Iran”, Phytopathol. Mediterr., vol. 53, no. 2, pp. 318–327, Sep. 2014.

Abstract

Symptoms similar to those of Pierce’s disease (PD) of grapevine and leaf scorch of almond were observed in vineyards and almond orchards in several provinces of Iran. Grafting of scions from symptomatic almond trees onto seedlings of a local almond (cv. Mamaee) under greenhouse conditions resulted in the transmission of the leaf scorch agent. A number of symptomatic samples from orchard and greenhouse plants were positive for presence of Xylella fastidiosa when tested by DAS-ELISA and PCR with X. fastidiosa specific antibodies and primers. A Gram-negative bacterium similar to X. fastidiosa was isolated on ‘periwinkle wilt’ (PW) medium. Selected isolates induced symptoms similar to those caused by X. fastidiosa when inoculated on Nicotiana tabacum, seedlings of almond and grapevine under greenhouse conditions. DAS-ELISA and PCR confirmed the identity of the isolated bacteria. On the basis of disease symptoms, graft transmission, isolation on specific X. fastidiosa culture medium, pathogenicity tests and positive reactions in DAS-ELISA and PCR, X. fastidiosa is associated with almond leaf scorch and Pierce’s disease in grapevine in Iran. This is the first report on the presence of X. fastidiosa in the Middle East and western Asia.

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