Vol. 53 No. 1 (2014)
Research Papers

Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy

Maryline MAGNIN-ROBERT
Université de Reims Champagne Ardenne, Laboratoire Stress, Défenses et Reproduction des Plantes, Reims
Alessandro SPAGNOLO
Université de Reims Champagne-Ardenne, Laboratoire Stress, Défenses et Reproduction des Plantes, Reims
Tchilabalo DILEZITOKO ALAYI
Université de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse Bioorganique
Clara CILINDRE
Université de Reims Champagne Ardenne, Laboratoire d’Œnologie et Chimie Appliquée
Laurence MERCIER
Moët & Chandon company
Christine SCHAEFFER-REISS
Université de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse Bioorganique
Alain VAN DORSSELAER
Université de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse Bioorganique
Christophe CLÉMENT
Université de Reims Champagne-Ardenne, Laboratoire Stress, Défenses et Reproduction des Plantes, Reims
Florence FONTAINE
Université de Reims Champagne-Ardenne, Laboratoire Stress, Défenses et Reproduction des Plantes, Reims
Published April 18, 2014
Keywords
  • grapevine,
  • proteomics,
  • qRT-PCR,
  • trunk diseases
How to Cite
[1]
M. MAGNIN-ROBERT, “Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy”, Phytopathol. Mediterr., vol. 53, no. 1, pp. 168-187, Apr. 2014.

Abstract

Among fungal grapevine trunk diseases, esca proper poses a significant threat for viticulture. Apoplexy, mainly occurring on grapevines affected by esca proper, is also a threat. To verify if different responses are activated in the woody tissues of apoplectic (A) and esca proper-affected (E) vines, two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine changes in the trunk wood of E and A field-grown plants. Asymptomatic and black streaked trunk (BST) wood from A and E plants were compared to asymptomatic and BST wood of control plants. Twenty-seven differentially expressed protein spots were identified. For eleven targeted proteins, expression of the relative transcripts was also monitored by qRT-PCR. Hierarchical tree clustering revealed differences in the distribution of spots containing carbohydrate metabolism proteins and heat shock proteins between asymptomatic- and BST-wood of grapevine, irrespective of the type of plant examined (control or diseased grapevines). Asymptomatic wood was mainly characterized by down-expression of proteins involved in cell growth and defense responses. The proteome of BST wood, characterized by extensive presence of grapevine trunk disease agents, revealed over-expression of proteins involved in defense. There was no evidence of strong different response in the trunk wood of apoplectic and esca proper affected plants. This could mean that, despite the different feature of these external crown symptoms, grapevine responses at trunk wood level is very similar in both cases. This plant response might therefore either simply be due to the fact that plants can react in the same way to different stresses, or that apoplexy represents a different effect provoked by the same cause or causal agent(s).

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...