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First report of strawberry mild yellow edge virus in Bosnia and Herzegovina

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  • Armin VUKOJEVIC,
  • Amani BEN SLIMEN,
  • Mirsad MUJKOVIC,
  • Emina KRAJINA-IBRULJ,
  • Toufic ELBEAINO
Armin VUKOJEVIC
Department of Quality Control of Seeds, Pest Diagnosis and the Presence of GMOs of Federal Institute of Agriculture, Sarajevo, Butmirska cesta 18, Ilidza 71210, Bosnia & Herzegovina
Amani BEN SLIMEN
Department of Integrated Pest Management, Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010 Valenzano, Bari, Italy
Mirsad MUJKOVIC
Department of Quality Control of Seeds, Pest Diagnosis and the Presence of GMOs of Federal Institute of Agriculture, Sarajevo, Butmirska cesta 18, Ilidza 71210, Bosnia & Herzegovina
Emina KRAJINA-IBRULJ
Department of Quality Control of Seeds, Pest Diagnosis and the Presence of GMOs of Federal Institute of Agriculture, Sarajevo, Butmirska cesta 18, Ilidza 71210, Bosnia & Herzegovina
Toufic ELBEAINO
Department of Integrated Pest Management, Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010 Valenzano, Bari, Italy
DOI: https://doi.org/10.36253/phyto-16227
Categories

Published 2025-12-11

Keywords

  • Strawberry,
  • DAS-ELISA,
  • RT-PCR,
  • sequence analysis

How to Cite

[1]
A. VUKOJEVIC, A. BEN SLIMEN, M. MUJKOVIC, E. KRAJINA-IBRULJ, and T. ELBEAINO, “First report of strawberry mild yellow edge virus in Bosnia and Herzegovina”, Phytopathol. Mediterr., pp. 579–583, Dec. 2025.

Copyright (c) 2025 Armin VUKOJEVIC, Amani BEN SLIMEN, Mirsad MUJKOVIC, Emina KRAJINA-IBRULJ, Toufic ELBEAINO

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

In 2023, a survey assessed 120 strawberry leaf samples collected from regions of Bosnia and Herzegovina (BiH) for presence of arabis mosaic virus (ArMV), strawberry mottle virus (SMoV), strawberry latent ringspot virus (SLRSV), and strawberry mild yellow edge virus (SMYEV), which are among the most common and internationally widespread viruses affecting strawberry plants. All samples were tested using Double Antibody Sandwich ELISA (DAS-ELISA) and RT-PCR. SMYEV was detected in 19 samples, 17 of which were from the variety Clery and two from Alba. ArMV, SLRSV, and SMoV were not detected in any of the samples. Sequence analyses of 405 nucleotides within the replicase and 25k triple gene block protein genes from 19 SMYEV isolates showed nine sequence variants. The least nucleotide similarity (83%) was observed with a Chinese SMYEV isolate, FJ1 (GenBank accession number OK562580), while greatest similarity (99.3%) was found with a German isolate, MY-18 (GenBank accession number NC_003794). Phylogenetic analysis showed that the BiH SMYEV isolates formed a distinct cluster closely related to the Argentinian isolate Berra-2 (GenBank accession number KX150372) and the German isolate, MY-18 (GenBank accession number NC_003794). This is the first report of SMYEV in BiH. Further research is required to determine whether additional viruses or other pathogens may be contributing to the symptoms observed in the field.

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