Vol. 63 No. 1 (2024)
Articles

Characterization and pathogenicity of Pleurostoma richardsiae causing decline of mango trees in Southern Italy

PDF
  • Giuseppa Rosaria LEONARDI,
  • Dalia AIELLO,
  • Giorgio GUSELLA,
  • Giancarlo POLIZZI
Giuseppa Rosaria LEONARDI
Dipartimento di Agricoltura, Alimentazione e Ambiente, University of Catania, 95123, Via S. Sofia 100, Catania
Dalia AIELLO
Dipartimento di Agricoltura, Alimentazione e Ambiente, University of Catania, 95123, Via S. Sofia 100, Catania
Giorgio GUSELLA
Dipartimento di Agricoltura, Alimentazione e Ambiente, University of Catania, 95123, Via S. Sofia 100, Catania
Giancarlo POLIZZI
Dipartimento di Agricoltura, Alimentazione e Ambiente, University of Catania, 95123, Via S. Sofia 100, Catania
DOI: https://doi.org/10.36253/phyto-15104

Published 2024-05-13

Keywords

  • Fungal diseases,
  • Mangifera indica,
  • wood necrosis,
  • twig dieback,
  • phylogeny

How to Cite

[1]
G. R. LEONARDI, D. AIELLO, G. GUSELLA, and G. POLIZZI, “Characterization and pathogenicity of Pleurostoma richardsiae causing decline of mango trees in Southern Italy”, Phytopathol. Mediterr., vol. 63, no. 1, pp. 111–118, May 2024.

Copyright (c) 2024 Giuseppa Rosaria LEONARDI, Dalia AIELLO, Giorgio GUSELLA, Giancarlo POLIZZI

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Mango trees (Mangifera indica) showing symptoms of twig and branch dieback, internal wood necroses, and decline, were surveyed in an orchard in Palermo province (Eastern Sicily, Italy). A Pleurostoma-like fungus was consistently isolated from symptomatic wood tissues. Based on morphology and phylogenetic analysis of ITS and tub2 sequences, the fungus was identified as Pleurostoma richardsiae. A pathogenicity test was conducted by inoculating stems of 2-year-old mango seedlings with mycelium plugs and conidium suspensions of a representative isolate. Two months after inoculation, necrotic lesions were observed around the inoculation points, and P. richardsiae was reisolated from the necrotic tissues. This is the first report of P. richardsiae causing dieback and decline of mango trees.

PDF

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

  1. Agustí-Brisach C., Jiménez-Urbano J.P., Raya M.C., López-Moral A., Trapero A., 2021. Vascular fungi associated with branch dieback of olive in superhigh-density systems in southern Spain. Plant Disease 105: 797–818. https://doi.org/10.1094/PDIS-08-20-1750-RE
  2. Ahmed Y., Cirvilleri G., D’Onghia A.M., Yaseen T., 2014. First report of Verticillium wilt of mango (Mangifera indica) caused by Verticillium dahliae in Italy. Plant Disease 98: 1156. https://doi.org/10.1094/PDIS-02-14-0130-PDN
  3. Aiello D., Guarnaccia V., Costanzo M.B., Leonardi G.R., Epifani F., … Polizzi G., 2022. Woody canker and shoot blight caused by Botryosphaeriaceae and Diaporthaceae on mango and litchi in Italy. Horticulturae 8: 330. https://doi.org/10.3390/horticulturae8040330
  4. Canale M.C., Nunes Nesi C., Falkenbach B.R., Hunhoff Da Silva C.A., Brugnara E.C., 2019. Pleurostomophora richardsiae associated with olive tree and grapevine decline in southern Brazil. Phytopatologia Mediterranea 58: 201–205. https://doi.org/10.13128/Phytopathol_Mediterr-23357
  5. Carlucci A., Raimondo M.L., Cibelli F., Phillips A.J.L., Lops F., 2013. Pleurostomophora richardsiae, Neofusicoccum parvum and Phaeoacremonium aleophilum associated with a decline of olives in southern Italy. Phytopathologia Mediterranea 52: 517–527. https://doi.org/10.14601/Phytopathol_Mediterr-13526
  6. Carlucci A., Cibelli F., Lops F., Phillips A.J.L., Ciccarone C., Raimondo M.L., 2015. Pleurostomophora richardsiae associated with trunk diseases of grapevines in southern Italy. Phytopatologia Mediterranea 54: 109–123. https://doi.org/10.14601/Phytopathol_Mediterr-15257
  7. García-Mahecha M., Soto-Valdez H., Carvajal-Millan E., Madera-Santana T.J., Lomelí-Ramírez M.G., Colín-Chávez C., 2023. Bioactive compounds in extracts from the agro-industrial waste of mango. Molecules 28(1): 458. https://doi.org/10.3390/molecules28010458
  8. Glass N.L., Donaldson G.C., 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied Environmental Microbiology 61: 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  9. Guarnaccia V., Kraus C., Markakis E.A., Alves A., Armengol J., … Gramaje D., 2023. Fungal trunk diseases of fruit trees in Europe: pathogens, spread and future directions. Phytopathologia Mediterranea 61: 563–599. https://doi.org/10.36253/phyto-14167
  10. Halleen F., Mostert L., Crous P.W., 2007. Pathogenicity testing of lesser-known vascular fungi of grapevines. Australasian Plant Pathology 36: 277–285. https://doi.org/10.1071/AP07019
  11. Ismail A.M., Cirvilleri G., Lombard L., Crous P.W., Groenewald J.Z., Polizzi G., 2013a. Characterisation of Neofusicoccum species causing mango dieback in Italy. Journal of Plant Pathology 95: 549–557. https://doi.org/10.4454/JPP.V95I3.008
  12. Ismail A.M., Cirvilleri G., Polizzi G., 2013b. Characterisation and pathogenicity of Pestalotiopsis uvicola and Pestalotiopsis clavispora causing grey leaf spot of mango (Mangifera indica L.) in Italy. European Journal of Plant Pathology 135: 619–625. https://doi.org/10.1007/s10658-012-0117-z
  13. Ismail A.M., Cirvilleri G., Yaseen T., Epifani F., Perrone G., Polizzi G., 2015. Characterisation of Colletotrichum species causing anthracnose disease of mango in Italy. Journal of Plant Pathology 97: 167–171. https://doi.org/10.4454/JPP.V97I1.011
  14. Ivic D., Tomic Z., Godena S., 2018. First report of Pleurostomophora richardsiae causing branch dieback and collar rot of olive in Istria, Croatia. Plant Disease 102: 2648. https://doi.org/10.1094/PDIS-04-18-0669-PDN
  15. Joachin J., Kritzell C., Lagueux E., Luecke N.C., Crawford K.M., 2023. Climate change and plant-microbe interactions: water-availability influences the effective specialization of a fungal pathogen. Fungal Ecology 66: 101286. https://doi.org/10.1016/j.funeco.2023.101286
  16. Kumar S., Stecher G., Li M., Knyaz C., Tamura K., 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
  17. Lauricella M., Emanuele S., Calvaruso G., Giuliano M., D’Anneo A., 2017. Multifaceted health benefits of Mangifera indica L. (mango): the inestimable value of orchards recently planted in Sicilian rural areas. Nutrients 9(5): 525. https://doi.org/10.3390/nu9050525
  18. Lawrence D.P., Nouri N.T., Trouillas F.P., 2021. Pleurostoma decline of olive trees caused by Pleurostoma richardsiae in California. Plant Disease 105(8): 2149–2159. https://doi.org/10.1094/PDIS-08-20-1771-RE
  19. Maharaj A., Naidoo Y., Dewir Y.H., Rihan H., 2022. Phytochemical screening and antibacterial and antioxidant activities of Mangifera indica leaves. Horticulturae 8: 909. https://doi.org/10.3390/horticulturae8100909
  20. Markakis E.A., Kavroulakis N., Ntougias S., Koubouris G.C., Sergentani C.K., Ligoxigakis E.K., 2017. Characterization of fungi associated with wood decay of tree species and grapevine in Greece. Plant Disease 101: 1929–1940. https://doi.org/10.1094/PDIS-12-16-1761-RE
  21. Mukherjee S.K., 1953. Origin, distribution and phylogenetic affinities of the species of Mangifera indica L. Journal of the Linnean Society of London, Botany 55: 65–83. https://doi.org/10.1111/j.1095-8339.1953.tb00004.x
  22. Nigro G., Boscia D., Antelmi I., Ippolito A., 2013. Fungal species associated with a severe decline of olive in southern Italy. Journal of Plant Pathology 95: 668. https://doi.org/10.4454/JPP.V95I3.034
  23. Olmo D., Armengol J., León M., Gramaje D., 2015. Pathogenicity testing of lesser-known fungal trunk pathogens associated with wood decay of almond trees. European Journal of Plant Pathology 143: 607–611. https://doi.org/10.1007/s10658-015-0699-3
  24. Pintos Varela G., Redondo Fernandez V., Aguin Casal O., Ferreiroa Martinez V., Mansilla Vazquez J.P., 2016. First report of Pleurostoma richardsiae causing grapevine trunk disease in Spain. Plant Disease 100: 2168. https://doi.org/10.1094/PDIS-05-11-0429
  25. Raimondo M.L., Carlucci A., Ciccarone C., Sadallah A., Lops F., 2019. Identification and pathogenicity of lignicolous fungi associated with grapevine trunk diseases in southern Italy. Phytopathologia Mediterranea 58(3): 639–662. https://doi.org/10.14601/Phyto-10742
  26. Rolshausen P.E., Urbez-Torres J.R., Rooney-Latham S., Eskalen A., Smith R.J., Gubler W.D., 2010. Evaluation of pruning wound susceptibility and protection against fungi associated with grapevine trunk diseases. American Journal of Enology and Viticulture 61: 113–119. https://doi.org/10.5344/ajev.2010.61.1.113
  27. Sohrabi M., Mohammadi H., León Santana M., Armengol F.J., Banihashemi Z., 2020. Fungal pathogens associated with branch and trunk cankers of nut crops in Iran. European Journal of Plant Pathology 157(2): 327–351. https://doi.org/10.1007/s10658-020-01996-w
  28. Spies C.F.J., Mostert L., Carlucci A., Moyo P., Van Jaarsveld W.J., … Halleen F., 2020. Dieback and decline pathogens of olive trees in South Africa. Persoonia 45: 196–220. https://doi.org/10.3767/persoonia.2020.45.08
  29. Swofford D.L., 2002. PAUP*: Phylogenetic analysis using parsimony (and other methods), version 4.0a165. Sinauer Associates, Sunderland, Massachusetts, USA.
  30. van Dyk M., Spies C.F., Mostert L., van der Rijst M., du Plessis I.L., … Halleen F., 2021. Pathogenicity testing of fungal isolates associated with olive trunk diseases in South Africa. Plant Disease 105: 4060–4073. https://doi.org/10.1094/PDIS-08-20-1837-RE
  31. Vijaykrishna D., Mostert L., Jeewon R., Gams W., Hyde K.D., Crous P.W., 2004. Pleurostomophora, an anamorph of Pleurostoma (Calpshaeriales), a new anamorphs genus morphologically similar to Phialophora. Studies in Mycology 50: 387–395. https://edepot.wur.nl/28946
  32. White T.J., Bruns T., Lee S.J.W.T., Taylor J.L., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a Guide to Methods andAapplications (M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White, ed.), Academic Press, New York, 315–321. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  33. White C.L., Halleen F., Mostert L., 2011. Symptoms and fungi associate with esca in South African vineyards. Phytopathologia Mediterranea 50: S236–S246. https://doi.org/10.14601/Phytopathol_Mediterr-8983

Most read articles by the same author(s)