Articles
Published 2021-09-13
Keywords
- Grapevine Trunk Diseases (GTDs),
- Botryosphaeria dieback,
- Botryosphaeriaceae
How to Cite
[1]
E. A. RANGEL-MONTOYA, M. PAOLINELLI, P. E. ROLSHAUSEN, C. VALENZUELA-SOLANO, and R. HERNANDEZ-MARTINEZ, “Characterization of Lasiodiplodia species associated with grapevines in Mexico”, Phytopathol. Mediterr., vol. 60, no. 2, pp. 237–251, Sep. 2021.
Abstract
Botryosphaeria dieback is one of the most prevalent grapevine trunk diseases (GTDs), and is caused by fungi in the Botryosphaeriaceae. Fungi invade grapevine vascular systems mainly through pruning wounds, and cause cankers and necrotic lesions, which lead to grapevine decline and death. Lasiodiplodia theobromae has been reported as a highly virulent pathogen of grapevine, and was previously reported in Mexican vineyards. The taxonomy of Lasiodiplodia was recently revised, adding new species, and some were reduced to synonymy. This study aimed to characterize Lasio-diplodia producing grapevine dieback symptoms in Sonora and Baja California, Mexico. Using the phylogenetic markers tef1-α and ITS regions, Lasiodiplodia brasiliensis, L. crassispora, L. exigua, and L. gilanensis were identified. Lasidiplodia exigua was the most prevalent species. Lasiodiplodia brasiliensis and L. gilanensis were very virulent to ‘Cabernet Sauvignon’ plants, while L. exigua and L. gilanensis were less virulent, and L. crassispora did not produce lesions at 2 months post-inoculation. The optimum temperature of the Lasiodiplodia spp. was 28°C, but all four species grew up to 37°C, and the isolates of L. exigua grew slowly at 40°C. This is the first report of the four of Lasio-diplodia species in vineyards of Mexico.
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References
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Agustí-Brisach C., León M., García-Jiménez J., Armengol J., 2015. Detection of grapevine fungal trunk pathogens on pruning shears and evaluation of their potential for spread of infection. Plant Disease 99: 976–981.
Akgül D.S., Savaş N.G., Özarslandan M., 2019. First Report of Wood Canker Caused by Lasiodiplodia exigua and Neoscytalidium novaehollandiae on Grapevine in Turkey. Plant Disease 103: 1036.
Alves A., Crous P.W., Correia A., Phillips A.J.L., Alves A., 2008. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28: 1–13.
Bertsch C., Ramírez‐Suero M., Magnin‐Robert M., Larignon P., Chong J., … Fontaine F., 2013. Grapevine trunk diseases: complex and still poorly understood. Plant Pathology 62: 243–265.
Billones-Baaijens R., Savocchia S., 2019. A review of Botryosphaeriaceae species associated with grapevine trunk diseases in Australia and New Zealand. Australasian Plant Pathology 48: 3–18.
Carbone I., Kohn L., 1999. A Method for Designing Primer Sets for Speciation Studies in Filamentous Ascomycetes. Mycologia 91: 553–556.
Correia K.C., Silva M.A., de Morais M.A., Jr. Armengol J., Phillips A.J.L., … Michereff S.J., 2016. Phylogeny, distribution and pathogenicity of Lasiodiplodia species associated with dieback of table grape in the main Brazilian exporting region. Plant Pathology 65: 92–103.
Cruywagen E.M., Slippers B., Roux J., Wingfield M.J., 2017. Phylogenetic species recognition and hybridisation in Lasiodiplodia: a case study on species from baobabs. Fungal Biology 121: 420–436.
de Silva N.I., Phillips A.J.L., Liu J.K., Lumyong S., Hyde K.D., 2019. Phylogeny and morphology of Lasiodiplodia species associated with Magnolia forest plants. Scientific reports 9: 1–11.
Dissanayake A.J., Phillips A.J.L., Li X.H., Hyde K.D., 2016. Botryosphaeriaceae: Current status of genera and species. Mycosphere 7: 1001–1073.
Fontaine F., Pinto C., Vallet J., Clément C., Gomes A.C., Spagnolo A., 2016. The effects of grapevine trunk diseases (GTDs) on vine physiology. European journal of plant pathology 144: 707–721.
García-Robles J.M., Tobón-Quijano J.I., Bringas-Taddei E., Mercado-Ruiz J.N., Luchsinger-Lagos L., Báez-Sañudo R., 2007. Daños y desórdenes fisiológicos en uva de mesa sonorense después del preenfriado y almacenamiento. Revista Iberoamericana de Tecnología Postcosecha 8: 89–100.
González-Andrade S., 2015. Cadena de valor económico del vino de Baja California, Mexico. Estududios fronterizos 16: 163–193.
Gramaje D., Armengol J., 2011. Fungal Trunck Pathogens in the grapevine propagation process Potencial inoculum sources, detection, identification, and management strategies. Plant Disease 95: 1040–1055.
Gramaje D., Úrbez-Torres J.R., Sosnowski M.R., 2018. Managing Grapevine Trunk Diseases with respect to etiology and epidemiology: Current Strategies and Future Prospects. Plant Disease 102: 12–39.
Gubler W.D., Rolshausen P.E., Trouillase F.P., Úrbez J.R., Voegel T., 2005. Grapevine trunk diseases in California. Practical Winery & Vineyard Jan/Feb: 6-25.
Hall T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic acids symposium series 41: 95–98.
Kumar S., Stecher G., Li M., Knyaz C., and Tamura K., 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular biology and evolution 35: 1547–1549.
Lawrence D.P., Travadon R., Nita M., Baumgartner K., 2017. TrunkDiseaseID. org: A molecular database for fast and accurate identification of fungi commonly isolated from grapevine wood. Crop Protection 102: 110–117.
Linaldeddu B.T., Deidda A., Scanu B., Franceschini A., Serra S., … Phillips A.J.L., 2015. Diversity of Botryosphaeriaceae species associated with grapevine and other woody hosts in Italy, Algeria and Tunisia, with descriptions of Lasiodiplodia exigua and Lasiodiplodia mediterranea sp. nov. Fungal Diversity 71: 201-214.
Mehl J., Wingfield M.J., Roux J., Slippers B., 2017. Invasive everywhere? Phylogeographic analysis of the globally distributed tree pathogen Lasiodiplodia theobromae. Forests 8: 1–22.
Netto M.S., Assunção I.P., Lima G.S., Marques M.W., Lima W.G., … Câmara M.P., 2014. Species of Lasiodiplodia associated with papaya stem-end rot in Brazil. Fungal Diversity 67: 127–141.
Obrador-Sánchez J.A., Hernandez-Martinez R., 2020. Microscope observations of Botryosphaeriaceae spp. in the presence of grapevine wood Phytopathologia Mediterranea 59: 119–129.
Phillips A.J.L., Alves A., Abdollahzadeh J., Slippers B., Wingfield, M.J., … Crous P.W., 2013. The Botryosphaeriaceae: genera and species known from culture. Studies in mycology 76: 51-167.
Punithalingam E., 1976. Botryodiplodia theobromae. IMI Descriptions of Fungi and Bacteria 519: 1–2.
Rolshausen P.E., Úrbez-Torres J.R., Rooney-Latham S., Eskalen A., Smith R.J., Gubler W.D., 2010. Evaluation of pruning wound susceptibility and protection against fungi associated with grapevine trunk diseases. American Journal of Enology and Viticulture 61: 113–119.
Rolshausen P.E., Akgül D.S., Perez R., Eskalen A., Gispert C., 2013. First report of wood canker caused by Neoscytalidium dimidiatum on grapevine in California. Plant Disease 97: 1511–1511.
SIAP Servicio de Información y Estadística Agroalimentaria y Pesquera, 2019. Ministerio de Agricultura de Mexico, Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA).
Slippers B., Smit W.A., Crous P.W., Coutinho T.A., Wingfield B.D., Wingfield M.J., 2007. Taxonomy, phylogeny and identification of Botryosphaeriaceae associated with pome and stone fruit trees in South Africa and other regions of the world. Plant Pathology 56: 128–139.
Stempien E., Goddard M.L., Wilhelm K., Tarnus C., Bertsch C., Chong, J., 2017. Grapevine Botryosphaeria dieback fungi have specific aggressiveness factor repertory involved in wood decay and stilbene metabolization. PloS one 12: e0188766.
Tamura K., 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution 9: 678–687.
Thompson J.D., Higgins D.G., Gibson T.J., 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids research 22: 4673–4680.
Tibpromma S., Hyde K.D., McKenzie E.H., Bhat D.J., Phillips A.J.L., … Karunarathna S.C., 2018. Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 93: 13–160.
Úrbez-Torres J.R., Leavitt G.M., Voegel T.M., Gubler W.D., 2006. Identification and Distribution of Botryosphaeria spp. Associated with Grapevine Cankers in California. Plant Disease 90: 1490–1503.
Úrbez-Torres J.R., Leavitt G.M., Guerrero J.C., Guevara J., Gubler W.D., 2008. Identification and pathogenicity of Lasiodiplodia theobromae and Diplodia seriata, the causal agents of bot canker disease of grapevines in Mexico. Plant Disease 92: 519–529.
Úrbez-Torres J.R., Gubler W.D., 2009. Pathogenicity of Botryosphaeriaceae Species Isolated from Grapevine Cankers in California. Plant Disease 93: 584–592.
Úrbez-Torres J.R., 2011. The status of Botryosphaeriaceae species infecting grapevines. Phytopathologia Mediterranea 50: S5–S45.
Wagner D.B., Furnier G.R., Saghai-Maroof M.A., Williams SM, Dancik B.P., Allard R.W., 1987. Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. PNAS 84: 2097–2100.
Waite H., Armengol J., Billones-Baaijens R., Gramaje D., Hallen F., … Smart R., 2018. A protocol for the management of grapevine rootstock mother vines to reduce latent infections by grapevine trunk pathogens in cuttings. Phytopathologia Mediterranea 57: 384–398.
White T.J., Bruns T., Lee S.J.W.T., Taylor J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315–322.
Agustí-Brisach C., León M., García-Jiménez J., Armengol J., 2015. Detection of grapevine fungal trunk pathogens on pruning shears and evaluation of their potential for spread of infection. Plant Disease 99: 976–981.
Akgül D.S., Savaş N.G., Özarslandan M., 2019. First Report of Wood Canker Caused by Lasiodiplodia exigua and Neoscytalidium novaehollandiae on Grapevine in Turkey. Plant Disease 103: 1036.
Alves A., Crous P.W., Correia A., Phillips A.J.L., Alves A., 2008. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28: 1–13.
Bertsch C., Ramírez‐Suero M., Magnin‐Robert M., Larignon P., Chong J., … Fontaine F., 2013. Grapevine trunk diseases: complex and still poorly understood. Plant Pathology 62: 243–265.
Billones-Baaijens R., Savocchia S., 2019. A review of Botryosphaeriaceae species associated with grapevine trunk diseases in Australia and New Zealand. Australasian Plant Pathology 48: 3–18.
Carbone I., Kohn L., 1999. A Method for Designing Primer Sets for Speciation Studies in Filamentous Ascomycetes. Mycologia 91: 553–556.
Correia K.C., Silva M.A., de Morais M.A., Jr. Armengol J., Phillips A.J.L., … Michereff S.J., 2016. Phylogeny, distribution and pathogenicity of Lasiodiplodia species associated with dieback of table grape in the main Brazilian exporting region. Plant Pathology 65: 92–103.
Cruywagen E.M., Slippers B., Roux J., Wingfield M.J., 2017. Phylogenetic species recognition and hybridisation in Lasiodiplodia: a case study on species from baobabs. Fungal Biology 121: 420–436.
de Silva N.I., Phillips A.J.L., Liu J.K., Lumyong S., Hyde K.D., 2019. Phylogeny and morphology of Lasiodiplodia species associated with Magnolia forest plants. Scientific reports 9: 1–11.
Dissanayake A.J., Phillips A.J.L., Li X.H., Hyde K.D., 2016. Botryosphaeriaceae: Current status of genera and species. Mycosphere 7: 1001–1073.
Fontaine F., Pinto C., Vallet J., Clément C., Gomes A.C., Spagnolo A., 2016. The effects of grapevine trunk diseases (GTDs) on vine physiology. European journal of plant pathology 144: 707–721.
García-Robles J.M., Tobón-Quijano J.I., Bringas-Taddei E., Mercado-Ruiz J.N., Luchsinger-Lagos L., Báez-Sañudo R., 2007. Daños y desórdenes fisiológicos en uva de mesa sonorense después del preenfriado y almacenamiento. Revista Iberoamericana de Tecnología Postcosecha 8: 89–100.
González-Andrade S., 2015. Cadena de valor económico del vino de Baja California, Mexico. Estududios fronterizos 16: 163–193.
Gramaje D., Armengol J., 2011. Fungal Trunck Pathogens in the grapevine propagation process Potencial inoculum sources, detection, identification, and management strategies. Plant Disease 95: 1040–1055.
Gramaje D., Úrbez-Torres J.R., Sosnowski M.R., 2018. Managing Grapevine Trunk Diseases with respect to etiology and epidemiology: Current Strategies and Future Prospects. Plant Disease 102: 12–39.
Gubler W.D., Rolshausen P.E., Trouillase F.P., Úrbez J.R., Voegel T., 2005. Grapevine trunk diseases in California. Practical Winery & Vineyard Jan/Feb: 6-25.
Hall T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic acids symposium series 41: 95–98.
Kumar S., Stecher G., Li M., Knyaz C., and Tamura K., 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular biology and evolution 35: 1547–1549.
Lawrence D.P., Travadon R., Nita M., Baumgartner K., 2017. TrunkDiseaseID. org: A molecular database for fast and accurate identification of fungi commonly isolated from grapevine wood. Crop Protection 102: 110–117.
Linaldeddu B.T., Deidda A., Scanu B., Franceschini A., Serra S., … Phillips A.J.L., 2015. Diversity of Botryosphaeriaceae species associated with grapevine and other woody hosts in Italy, Algeria and Tunisia, with descriptions of Lasiodiplodia exigua and Lasiodiplodia mediterranea sp. nov. Fungal Diversity 71: 201-214.
Mehl J., Wingfield M.J., Roux J., Slippers B., 2017. Invasive everywhere? Phylogeographic analysis of the globally distributed tree pathogen Lasiodiplodia theobromae. Forests 8: 1–22.
Netto M.S., Assunção I.P., Lima G.S., Marques M.W., Lima W.G., … Câmara M.P., 2014. Species of Lasiodiplodia associated with papaya stem-end rot in Brazil. Fungal Diversity 67: 127–141.
Obrador-Sánchez J.A., Hernandez-Martinez R., 2020. Microscope observations of Botryosphaeriaceae spp. in the presence of grapevine wood Phytopathologia Mediterranea 59: 119–129.
Phillips A.J.L., Alves A., Abdollahzadeh J., Slippers B., Wingfield, M.J., … Crous P.W., 2013. The Botryosphaeriaceae: genera and species known from culture. Studies in mycology 76: 51-167.
Punithalingam E., 1976. Botryodiplodia theobromae. IMI Descriptions of Fungi and Bacteria 519: 1–2.
Rolshausen P.E., Úrbez-Torres J.R., Rooney-Latham S., Eskalen A., Smith R.J., Gubler W.D., 2010. Evaluation of pruning wound susceptibility and protection against fungi associated with grapevine trunk diseases. American Journal of Enology and Viticulture 61: 113–119.
Rolshausen P.E., Akgül D.S., Perez R., Eskalen A., Gispert C., 2013. First report of wood canker caused by Neoscytalidium dimidiatum on grapevine in California. Plant Disease 97: 1511–1511.
SIAP Servicio de Información y Estadística Agroalimentaria y Pesquera, 2019. Ministerio de Agricultura de Mexico, Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA).
Slippers B., Smit W.A., Crous P.W., Coutinho T.A., Wingfield B.D., Wingfield M.J., 2007. Taxonomy, phylogeny and identification of Botryosphaeriaceae associated with pome and stone fruit trees in South Africa and other regions of the world. Plant Pathology 56: 128–139.
Stempien E., Goddard M.L., Wilhelm K., Tarnus C., Bertsch C., Chong, J., 2017. Grapevine Botryosphaeria dieback fungi have specific aggressiveness factor repertory involved in wood decay and stilbene metabolization. PloS one 12: e0188766.
Tamura K., 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution 9: 678–687.
Thompson J.D., Higgins D.G., Gibson T.J., 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids research 22: 4673–4680.
Tibpromma S., Hyde K.D., McKenzie E.H., Bhat D.J., Phillips A.J.L., … Karunarathna S.C., 2018. Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 93: 13–160.
Úrbez-Torres J.R., Leavitt G.M., Voegel T.M., Gubler W.D., 2006. Identification and Distribution of Botryosphaeria spp. Associated with Grapevine Cankers in California. Plant Disease 90: 1490–1503.
Úrbez-Torres J.R., Leavitt G.M., Guerrero J.C., Guevara J., Gubler W.D., 2008. Identification and pathogenicity of Lasiodiplodia theobromae and Diplodia seriata, the causal agents of bot canker disease of grapevines in Mexico. Plant Disease 92: 519–529.
Úrbez-Torres J.R., Gubler W.D., 2009. Pathogenicity of Botryosphaeriaceae Species Isolated from Grapevine Cankers in California. Plant Disease 93: 584–592.
Úrbez-Torres J.R., 2011. The status of Botryosphaeriaceae species infecting grapevines. Phytopathologia Mediterranea 50: S5–S45.
Wagner D.B., Furnier G.R., Saghai-Maroof M.A., Williams SM, Dancik B.P., Allard R.W., 1987. Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. PNAS 84: 2097–2100.
Waite H., Armengol J., Billones-Baaijens R., Gramaje D., Hallen F., … Smart R., 2018. A protocol for the management of grapevine rootstock mother vines to reduce latent infections by grapevine trunk pathogens in cuttings. Phytopathologia Mediterranea 57: 384–398.
White T.J., Bruns T., Lee S.J.W.T., Taylor J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315–322.