Characterization of Neopestalotiopsis, Pestalotiopsis and Truncatella species associated with grapevine trunk diseases in France

Abstract

Pestalotioid fungi associated with grapevine wood diseases in France are regularly found in vine growing regions, and research was conducted to identify these fungi. Many of these taxa are morphologically indistinguishable, but sequence data can resolve the cryptic species in the group. Thirty pestalotioid fungi were isolated from infected grapevines from seven field sites and seven diseased grapevine varieties in France. Analysis of internal transcribed spacer (ITS), partial β-tubulin (TUB) and partial translation elongation factor 1-alpha (TEF) sequence data revealed several species of Neopestalotiopsis, Pestalotiopsis and Truncatella associated with the symptoms. Three Neopestalotiopsis spp. and one Pestalotiopsis sp. are reported for the first time associated with wood diseases in grapevine in France and worldwide, and include Neopestalotiopsis asiatica, N. javaensis, Neopestalotiopsis sp. and Pestalotiopsis biciliata. The sequence data indicate that Truncatella angustata was also associated with wood grapevine diseases in France; this species was previously reported on grapevine in Iran.  The importance of controlling Pestalotioid fungi associated with wood grapevine diseases is discussed, which was previously considered of minor importance. These fungi are isolated from plants in nurseries before marketing.