Research Papers
Construction and evaluation of a transformant library of Lasiodiplodia theobromae generated through Restriction Enzyme-Mediated Integration
Published 2014-12-22
Keywords
- mutants,
- grapevine Botryosphaeria dieback
How to Cite
[1]
W. ZHANG, “Construction and evaluation of a transformant library of Lasiodiplodia theobromae generated through Restriction Enzyme-Mediated Integration”, Phytopathol. Mediterr., vol. 53, no. 3, pp. 451–458, Dec. 2014.
Copyright (c) 2014 Wei ZHANG, Aifen LIU, Xin ZHANG, Hong YAN, Ruiqi LIU, Qianru PANG, Jinguang HUANG, Xinghong LI, Jiye YAN
This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related genes. Lasiodiplodia theobromae strain JZB 0300251, a highly virulent isolate, was selected for the genetic transformation. Based on optimised conditions, the Restriction Enzyme-Mediated Integration (REMI) methodology was established in L. theobromae using pUCATPH (a plasmid carrying a hygromycin B marker). A total of 6,036 transformants were generated with four restriction enzymes, respectively and the transformant library was evaluated based on 200 randomly selected transformants. Mutants that exhibited various degrees of virulence and different growth rates were obtained. The study provides basic results that will lead to increased understanding of the role of the pathogenicity-related genes involved in the infection process of L. theobromae.Downloads
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