Research Papers
Heterosporicola beijingense sp. nov. (Leptosphaeriaceae, Pleosporales) associated with Chenopodium quinoa leaf spots
Published 2020-07-17
Keywords
- DNA analyses,
- Pathogens,
- morpho-molecular taxonomy
How to Cite
[1]
R. S. BRAHMANAGE, “Heterosporicola beijingense sp. nov. (Leptosphaeriaceae, Pleosporales) associated with Chenopodium quinoa leaf spots”, Phytopathol. Mediterr., vol. 59, no. 2, pp. 219–227, Jul. 2020.
Abstract
A coelomycetous fungus with hyaline, aseptate, oblong to ellipsoidal conidia was isolated from living Chenopodium quinoa leaves with leaf spots, in Beijing, China. Maximum likelihood and Bayesian analyses of a combined LSU, SSU, ITS and TEF sequence dataset confirmed its placement in Heterosporicola in Leptosphaeriaceae. The new taxon resembles other Heterosporicola species, but is phylogenetically distinct, and is introduced as a new species. Heterosporicola beijingense sp. nov. is compared with other Heterosporicola species, and comprehensive descriptions and micrographs are provided.
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References
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Brahmanage R.S., D.N. Wanasinghe, M.C. Dayarathne, R. Jeewon, J. Yan, T.S. Bulgakov, E Camporesi, P Kakumyan, K.D. Hyde and X. Li 2019. Morphology and phylogeny reveal Stemphylium dianthi sp. nov. and new host records for the sexual morphs of S. beticola, S. gracilariae, S. simmonsii and S. vesicarium from Italy and Russia. Phytotaxa 411, 243–263.
Carbone I. and L.M. Kohn, 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91, 553–556.
Dayarathne M.C., R. Phookamsak, H.A. Ariyawansa, E.B.G. Jones, E. Camporesi and K.D. Hyde, 2015. Phylogenetic and morphological appraisal of Leptosphaeria italica sp. nov. (Leptosphaeriaceae, Pleosporales) from Italy. Mycosphere 6, 634–642.
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Jeewon R. and K.D. Hyde, 2016. Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7, 1669–1677.
Kuraku S., C.M. Zmasek, O. Nishimura and K. Katoh, 2013. A Leaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity. Nucleic acids research 41(W1), W22–W28.
Lee M.S., Y.L. Yang, C.Y. Wu, Y.L. Chen, C.K. Lee, S.S. Tzean and T.H. Lee, 2019. Efficient identification of fungal antimicrobial principles by tandem MS and NMR database. Journal of Food and Drug Analysis 27, 860-868.
Li J., X. Zhou, H. Huang and G. Li, 2017. Diseases characteristic and control measurements for Chenopodium quinoa Willd. In 2017 6th International Conference on Energy and Environmental Protection (ICEEP 2017). Atlantis Press. Miller M.A., W. Pfeiffer and T. Schwartz, 2010. Creating the CIPRES science gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE) 1, 1–8.
Nylander, J.A.A. 2004. MrModeltest 2.2: Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Sweden.
Rambaut A. 2009. FigTree, v. 1.4. 0, 2006–2012.
Rannala B., J. Huelsenbeck, Z. Yang and R. Nielsen 1998. Taxon Sampling and the Accuracy of Large Phylogenies. Systematic Biology 47, 702–710.
Rehner, S.A. and E.P. Buckley, 2005. A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97, 84–98.
Ronquist, F. and J.P. Huelsenbeck, 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.
Ronquist, F. M., Teslenko, P. Van Der Mark, D.L. Ayres, A. Darling, S. Höhna, B. Larget, L. Liu, M.A. Suchard and J.P. Huelsenbeck 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539–542.
Stamatakis, A., 2014. RAxML version 8, a tool for phylogenetic analysis and post–analysis of large phylogenies. Bioinformatics 30, 1312–1313.
Tapia, M., 1997. Cultivos andinos subexplotados y su aporte a la alimentacio´n. Santiago, Chile: FAO-RLAC: 99–103.
Tennakoon, D.S., R. Phookamsak, D.N. Wanasinghe, J.B. Yang, S. Lumyong, and K.D. Hyde, 2017. Morphological and phylogenetic insights resolve Plenodomus sinensis (Leptosphaeriaceae) as a new species. Phytotaxa 324, 73–82.
Testen, A.T., M.D.M. Jiménez-Gasco, J.B. Ochoa and P.A. Backman, 2013. Molecular detection of Peronospora variabilis in quinoa seed and phylogeny of the quinoa downy mildew pathogen in South America and the United States. Plant Disease 104, 379–386.
Valencia-Chamorro, S.A., 2003. Quinoa in Encyclopedia of Food Science
and Nutrition, ed. by Caballero B. Academic Press, Amsterdam, 4895–4902 pp.
Van der Aa, H.A. and H.A. Van Kesteren, 1979. Some pycnidial fungi occurring on Atriplex and Chenopodium. Persoonia-Molecular Phylogeny and Evolution of Fungi 10, 267–276.
Vega-Galvez, A., M. Miranda, J. Vergara, E. Uribe, L. Puente and E.A. Martinez,
2010. Nutrition facts and functional potential of quinoa (Chenopodium quinoa willd.), an ancient Andean grain: a review. Journal of the Science of Food and Agriculture 90, 2541–2547.
Vilca, A., 1972. Estudio de la mancha foliar en quinua (Doctoral dissertation, Tesis. Ing. Agro. Puno, Perú. Universidad Nacional Técnica del Altiplano)
Vilgalys, R. and M. Hester, 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172, 4238–4246.
Wang C. X. Zhao, G. Lu and Q. Mao, 2014. A review of characteristics and utilization of Chenopodium quinoa. Journal of Zhejiang A&F University 31, 296–301.
White T., T. Bruns, S. Lee and J. Taylor, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols, A Guide to Methods and Applications 18, 315–322.
Wijayawardene N.N., K.D. Hyde, H.T. Lumbsch, J.K. Liu, S.S. Maharachchikumbura, A.H. Ekanayaka, Q. Tian and R. Phookamsak, 2018. Outline of Ascomycota, 2017. Fungal Diversity 88, 167–263.
Wijayawardene N.N., M. Papizadeh, A.J.L. Phillips, D.N. Wanasinghe, D.J. Bhat, H.L.D. Weerahewa, B.D. Shenoy, Y. Wang and Y.Q. Huang 2017. Mycosphere Essays 19: Recent advances and future challenges in taxonomy of coelomycetous fungi. Mycosphere 8, 934–950.
Wright K.H., O.A. Pike, D.J. Fairbanks and S.C. Huber, 2002. Composition of Atriplex hortensis, sweet and bitter Chenopodium quinoa seeds. Food Chemical Toxicology 67, 1383–1385.
Zhaxybayeva O. and J.P. Gogarten, 2002. Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses. BMC genomics 3, 4.
Alandia S.O., V. tazu and B. Salas, 1979. Enfermedades. In: Tapia M, Gandarillas H, Alandia S, Cardozo A, Mujica A, Orti R, Otazu V, Rea J, Salas B, Sanabria E, eds. Quinua y Kan˜iwa. Bogota´ Colombia: IICA, 137–148 pp.
Aveskamp M.M., J. De Gruyter, J.H.C. Woudenberg, G.J.M. Verkley and P.W. Crous, 2010. Highlights of the Didymellaceae: a polyphasic approach to characterize Phoma and related pleosporalean genera. Studies in Mycology 65, 1–60.
Alves J.L., J.H.C. Woudenberg, L.L. Durate, P.W. Crousand, R.W. Barreto, 2013. Reappraisal of the genus Alternariaster (Dothideomycetes). Persoonia 31, 77–85.
Boerema G.H., S.B. Mathur and P. Neergaard, 1977. Ascochyta hyalospora (Cooke &Ell.) comb. nov. in seeds of Chenopodium quinoa. Netherlands Journal of Plant Pathology 83, 153–159.
Boerema G.H., 1997. Contributions toward a monograph of Phoma (coelomycetes) V. Subdivision of the genus in sections. Mycotaxon 64, 321–333.
Brahmanage R.S., D.N. Wanasinghe, M.C. Dayarathne, R. Jeewon, J. Yan, T.S. Bulgakov, E Camporesi, P Kakumyan, K.D. Hyde and X. Li 2019. Morphology and phylogeny reveal Stemphylium dianthi sp. nov. and new host records for the sexual morphs of S. beticola, S. gracilariae, S. simmonsii and S. vesicarium from Italy and Russia. Phytotaxa 411, 243–263.
Carbone I. and L.M. Kohn, 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91, 553–556.
Dayarathne M.C., R. Phookamsak, H.A. Ariyawansa, E.B.G. Jones, E. Camporesi and K.D. Hyde, 2015. Phylogenetic and morphological appraisal of Leptosphaeria italica sp. nov. (Leptosphaeriaceae, Pleosporales) from Italy. Mycosphere 6, 634–642.
De Gruyter J, J.H.C. Woudenberg, M.M. Aveskamp, G.J.M. Verkley, J.Z. Groenewald and P.W. Crous, 2013. Redisposition of Phoma-like anamorphs in Pleosporales. Studies in Mycology 75, 1–36.
Dini A, L. Rastrelli, P. Saturnino and O. Schettino, 1992. A compositional study of Chenopodium quinoa seeds. Nahrung 36, 400–404.
Hall T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In Nucleic acids symposium series 41, 95–98.
Huelsenbeck J.P. and F. Ronquist, 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754–755.
https://www.ncbi.nlm.nih.gov/nucleotide (accessed: January 2020).
Hyde K.D., N. Chaiwan, C. Norphanphoun, S. Boonmee, E. Camporesi, K.W.T. Chethana, M.C. Dayarathne, N.I. de Silva, A.J. Dissanayake, A.H. Ekanayaka, S. Hongsanan, S.K. Huang, S.C. Jayasiri, R. Jayawardena, H.B. Jiang, A. Karunarathna, C.G. Lin, J.K. Liu, N.G. Liu, Y.Z. Lu, Z.L. Luo, S.S.N. Maharachchimbura, I.S. Manawasinghe, D. Pem, R.H. Perera, C. Phukhamsakda, M.C. Samarakoon, C. Senwanna, Q.J. Shang, D.S. Tennakoon, K.M. Thambugala, S. Tibpromma, D.N. Wanasinghe, Y.P. Xiao, J. Yang, X.Y. Zeng, J.F. Zhang, S.N. Zhang, T.S. Bulgakov, D.J. Bhat, R. Cheewangkoon, T.K. Goh, E.B.G. Jones, J.C. Kang, R. Jeewon, Z.Y. Liu, S. Lumyong, C.H. Kuo, E.H.C. McKenzie, T.C. Wen, J.Y. Yan and Q. Zhao, 2018. Mycosphere notes 169–224. Mycosphere 9, 271–430.
Index Fungorum, 2020. http://www.indexfungorum.org/Names/Names.asp. (accessed: January 2020).
Jayasiri S.C., K.D. Hyde, H.A. Ariyawansa, J. Bhat, B. Buyck, L. Cai, Y.C. Dai, K.A. Abd-Elsalam, D. Ertz, I. Hidayat, R. Jeewon, E.B.G. Jones, A.H. Bahkali, S.C. Karunarathna, J.K. Liu, J.J. Luangsa-ard, H.T. Lumbsch, S.S.N. Maharachchikumbura, E.H.C. McKenzie, J.M. Moncalvo, M. Ghobad-Nejhad, H. Nilsson, K.A. Pang, O.L. Pereira, A.J.L. Phillips, O. Raspé, A.W. Rollins, A.I. Romero, J. Etayo, F. Selçuk, S.L. Stephenson, S. Suetrong, J.E. Taylor, C.K.M. Tsui, A. Vizzini, M.A. Abdel-Wahab, T.C. Wen, S. Boonmee, D.Q. Dai, D.A. Daranagama, A.J. Dissanayake, A.H. Ekanayaka, S.C. Fryar, S. Hongsanan, R.S. Jayawardena, W.J. Li, R.H. Perera, R. Phookamsak, N.I. de Silva, K.M. Thambugala, Q. Tian, N.N. Wijayawardene, R.L. Zhao, Q. Zhao, J.C. Kang and I. Promputtha, 2015. The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74, 3–18.
Jeewon R. and K.D. Hyde, 2016. Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7, 1669–1677.
Kuraku S., C.M. Zmasek, O. Nishimura and K. Katoh, 2013. A Leaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity. Nucleic acids research 41(W1), W22–W28.
Lee M.S., Y.L. Yang, C.Y. Wu, Y.L. Chen, C.K. Lee, S.S. Tzean and T.H. Lee, 2019. Efficient identification of fungal antimicrobial principles by tandem MS and NMR database. Journal of Food and Drug Analysis 27, 860-868.
Li J., X. Zhou, H. Huang and G. Li, 2017. Diseases characteristic and control measurements for Chenopodium quinoa Willd. In 2017 6th International Conference on Energy and Environmental Protection (ICEEP 2017). Atlantis Press. Miller M.A., W. Pfeiffer and T. Schwartz, 2010. Creating the CIPRES science gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE) 1, 1–8.
Nylander, J.A.A. 2004. MrModeltest 2.2: Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Sweden.
Rambaut A. 2009. FigTree, v. 1.4. 0, 2006–2012.
Rannala B., J. Huelsenbeck, Z. Yang and R. Nielsen 1998. Taxon Sampling and the Accuracy of Large Phylogenies. Systematic Biology 47, 702–710.
Rehner, S.A. and E.P. Buckley, 2005. A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97, 84–98.
Ronquist, F. and J.P. Huelsenbeck, 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.
Ronquist, F. M., Teslenko, P. Van Der Mark, D.L. Ayres, A. Darling, S. Höhna, B. Larget, L. Liu, M.A. Suchard and J.P. Huelsenbeck 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539–542.
Stamatakis, A., 2014. RAxML version 8, a tool for phylogenetic analysis and post–analysis of large phylogenies. Bioinformatics 30, 1312–1313.
Tapia, M., 1997. Cultivos andinos subexplotados y su aporte a la alimentacio´n. Santiago, Chile: FAO-RLAC: 99–103.
Tennakoon, D.S., R. Phookamsak, D.N. Wanasinghe, J.B. Yang, S. Lumyong, and K.D. Hyde, 2017. Morphological and phylogenetic insights resolve Plenodomus sinensis (Leptosphaeriaceae) as a new species. Phytotaxa 324, 73–82.
Testen, A.T., M.D.M. Jiménez-Gasco, J.B. Ochoa and P.A. Backman, 2013. Molecular detection of Peronospora variabilis in quinoa seed and phylogeny of the quinoa downy mildew pathogen in South America and the United States. Plant Disease 104, 379–386.
Valencia-Chamorro, S.A., 2003. Quinoa in Encyclopedia of Food Science
and Nutrition, ed. by Caballero B. Academic Press, Amsterdam, 4895–4902 pp.
Van der Aa, H.A. and H.A. Van Kesteren, 1979. Some pycnidial fungi occurring on Atriplex and Chenopodium. Persoonia-Molecular Phylogeny and Evolution of Fungi 10, 267–276.
Vega-Galvez, A., M. Miranda, J. Vergara, E. Uribe, L. Puente and E.A. Martinez,
2010. Nutrition facts and functional potential of quinoa (Chenopodium quinoa willd.), an ancient Andean grain: a review. Journal of the Science of Food and Agriculture 90, 2541–2547.
Vilca, A., 1972. Estudio de la mancha foliar en quinua (Doctoral dissertation, Tesis. Ing. Agro. Puno, Perú. Universidad Nacional Técnica del Altiplano)
Vilgalys, R. and M. Hester, 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172, 4238–4246.
Wang C. X. Zhao, G. Lu and Q. Mao, 2014. A review of characteristics and utilization of Chenopodium quinoa. Journal of Zhejiang A&F University 31, 296–301.
White T., T. Bruns, S. Lee and J. Taylor, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols, A Guide to Methods and Applications 18, 315–322.
Wijayawardene N.N., K.D. Hyde, H.T. Lumbsch, J.K. Liu, S.S. Maharachchikumbura, A.H. Ekanayaka, Q. Tian and R. Phookamsak, 2018. Outline of Ascomycota, 2017. Fungal Diversity 88, 167–263.
Wijayawardene N.N., M. Papizadeh, A.J.L. Phillips, D.N. Wanasinghe, D.J. Bhat, H.L.D. Weerahewa, B.D. Shenoy, Y. Wang and Y.Q. Huang 2017. Mycosphere Essays 19: Recent advances and future challenges in taxonomy of coelomycetous fungi. Mycosphere 8, 934–950.
Wright K.H., O.A. Pike, D.J. Fairbanks and S.C. Huber, 2002. Composition of Atriplex hortensis, sweet and bitter Chenopodium quinoa seeds. Food Chemical Toxicology 67, 1383–1385.
Zhaxybayeva O. and J.P. Gogarten, 2002. Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses. BMC genomics 3, 4.