Vol. 53 No. 1 (2014)
Research Papers

The Italian inter-laboratory study on the detection of <em>Pseudomonas syringae</em> pv. <em>actinide</em>

Stefania LORETI
Consiglio per la ricerca e la sperimentazione in agricoltura [Centro di ricerca per la patologia vegetale, CRA-PAV] [Plant Pathology Research Center]
Nicoletta PUCCI
Consiglio per la ricerca e la sperimentazione in agricoltura [Centro di ricerca per la patologia vegetale, CRA-PAV] [Plant Pathology Research Center]
Angela GALLELLI
Consiglio per la ricerca e la sperimentazione in agricoltura [Centro di ricerca per la patologia vegetale, CRA-PAV] [Plant Pathology Research Center]
Paola MINARDI
Department of Veterinary Medical Sciences – DiMeVet Alma Mater Studiorum - University of Bologna, Via Tolara di Sopra 50, I - 40064 Ozzano Emilia (BO), Italy
Stefano ARDIZZI
Department of Agricultural Sciences – DipSA - University of Bologna, Viale Fanin 42, 40127 Bologna, Italy
Giorgio BALESTRA
Dipartimento di Scienze e tecnologie per l’agricoltura, le foreste, la natura e l’energia (DAFNE), Via S. Camillo De Lellis snc - 01100 Viterbo, Italy
Angelo MAZZAGLIA
Dipartimento di Scienze e tecnologie per l’agricoltura, le foreste, la natura e l’energia (DAFNE), Via S. Camillo De Lellis snc - 01100 Viterbo, Italy
Maria TARATUFOLO
Dipartimento di Scienze e tecnologie per l’agricoltura, le foreste, la natura e l’energia (DAFNE), Via S. Camillo De Lellis snc - 01100 Viterbo, Italy
Published April 18, 2014
Keywords
  • kiwifruit bacterial canker,
  • symptomatic/symptomless host material,
  • dilution plating
How to Cite
[1]
S. LORETI, “The Italian inter-laboratory study on the detection of <em>Pseudomonas syringae</em> pv. <em>actinide</em&gt;”, Phytopathol. Mediterr., vol. 53, no. 1, pp. 159-167, Apr. 2014.

Abstract

A severe form of bacterial canker of kiwifruit, caused by Pseudomonas syringae pv. actinidiae (Psa), has been detected in all the main areas of cultivation of kiwifruit (Actinidia deliciosa and A. chinensis). Since 2010 several research groups have been assessing methods and procedures to detect and identify Psa, both from symptomatic and symptomless host material. In 2011, a study to compare Psa diagnostic methods was performed with reference to Psa strains and related pathovars, and with plant extracts or DNA obtained from healthy and naturally infected leaves, pollen or wood. The study revealed the strengths and the weaknesses of the assessed methods. The procedure included screening tests for Psa detection and for identification of Psa colonies. The methods assessed were bacterial isolation on generic and semi-selective media, PCR analysis (single, duplex and rep-PCR assay, the latter for identification only). The results highlighted the best performance of semi-selective with respect the generic media; the usefulness of the direct-PCR as screening tests for Psa detection; and the greater specificity of duplex-PCR and sensitivity of simple-PCR. The use of semi-selective medium for isolation and of two PCR-based methods - in parallel - for Psa detection are suggested. Both rep-PCR and duplex-PCR, were found to be specific, and are recommended as an identification test for this pathogen.

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