Vol. 57 No. 1 (2018)
Research Papers

A zinc, copper and citric acid biocomplex shows promise for control of Xylella fastidiosa subsp. pauca in olive trees in Apulia region (southern Italy)

Marco SCORTICHINI
Council for Agricultural Research and the Analysis of Agricultural Economics (CREA), Research Centre for Olive, Fruit Trees and Citrus, Via Torrino 3, I-81100 Caserta, Italy
Jianchi CHEN
U.S. Department of Agriculture, Agricultural Research Service, 93648 Parlier (CA), USA
Monica DE CAROLI
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A), University of Salento, Campus Ecoteckne, Via Prov.le Lecce-Monteroni, I-73100 Lecce, Italy
Giuseppe DALESSANDRO
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A), University of Salento, Campus Ecoteckne, Via Prov.le Lecce-Monteroni, I-73100 Lecce, Italy
Nicoletta PUCCI
Council for Agricultural Research and the Analysis of Agricultural Economics (CREA), Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, I-00156 Roma, Italy
Vanessa MODESTI
Council for Agricultural Research and the Analysis of Agricultural Economics (CREA), Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, I-00156 Roma, Italy
Alessia L’AURORA
Council for Agricultural Research and the Analysis of Agricultural Economics (CREA), Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, I-00156 Roma, Italy
Milena PETRICCIONE
Council for Agricultural Research and the Analysis of Agricultural Economics (CREA), Research Centre for Olive, Fruit Trees and Citrus, Via Torrino 3, I-81100 Caserta, Italy
Luigi ZAMPELLA
Council for Agricultural Research and the Analysis of Agricultural Economics (CREA), Research Centre for Olive, Fruit Trees and Citrus, Via Torrino 3, I-81100 Caserta, Italy
Francesco MASTROBUONI
Council for Agricultural Research and the Analysis of Agricultural Economics (CREA), Research Centre for Olive, Fruit Trees and Citrus, Via Torrino 3, I-81100 Caserta, Italy
Danilo MIGONI
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A), University of Salento, Campus Ecoteckne, Via Prov.le Lecce-Monteroni, I-73100 Lecce, Italy
Laura DEL COCO
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A), University of Salento, Campus Ecoteckne, Via Prov.le Lecce-Monteroni, I-73100 Lecce, Italy
Chiara Roberta GIRELLI
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A), University of Salento, Campus Ecoteckne, Via Prov.le Lecce-Monteroni, I-73100 Lecce, Italy
Filippo PIACENTE
AgriBioTech, Viale Unità d’Italia 450/10, I-74122 Talsano (TA), Italy
Filippo PIACENTE
AgriBioTech, Viale Unità d’Italia 450/10, I-74122 Talsano (TA), Italy
Nicola CRISTELLA
Studio Agro-Ambientale e Ingegneria Terranostra srls, Via XXIV Maggio 10, I-74020 Lizzano (TA), Italy
Paolo MARANGI
Studio Agro-Ambientale e Ingegneria Terranostra srls, Via XXIV Maggio 10, I-74020 Lizzano (TA), Italy
Francesco LADDOMADA
Volare Alto srls, Via Pirandello 31, I-74012 Crispiano (TA), Italy
Michele DI CESARE
Volare Alto srls, Via Pirandello 31, I-74012 Crispiano (TA), Italy
Gianluigi CESARI
NEPRI srl, Start up Innovazione e Divulgazione Ricerca in Agricultura, Via M. Cifarelli 6/A, I-70124 Bari, Italy
Francesco Paolo FANIZZI
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A), University of Salento, Campus Ecoteckne, Via Prov.le Lecce-Monteroni, I-73100 Lecce, Italy
Stefania LORETI
Council for Agricultural Research and the Analysis of Agricultural Economics (CREA), Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, I-00156 Roma, Italy
Published March 14, 2018
Keywords
  • Olive Quick Decline Syndrome,
  • quarantine bacteria,
  • endotherapy,
  • confocal laser scanning microscopy,
  • quantitative real-time PCR
How to Cite
[1]
M. SCORTICHINI, “A zinc, copper and citric acid biocomplex shows promise for control of Xylella fastidiosa subsp. pauca in olive trees in Apulia region (southern Italy)”, Phytopathol. Mediterr., vol. 57, no. 1, pp. 48-72, Mar. 2018.

Abstract

The bacterium Xylella fastidiosa subsp. pauca is associated with the “olive quick decline syndrome” in the Apulia region of southern Italy. To investigate control of this phytopathogen, a compound containing zinc and copper complexed with citric-acid hydracids (Dentamet®) was evaluated for in vitro and in planta bactericidal activity. Confocal laser scanning microscopy, fluorescent quantification and atomic emission spectroscopy were then used to determine if the compound reached the xylem networks of leaves, twigs and branches of olive, to release zinc and copper within the xylem. A 3-year field trial in an olive orchard containing mature Cellina di Nardò and Ogliarola salentina olive trees, and officially declared infected by X. fastidiosa subsp. pauca,was also carried out o to determine if the compound affected severity of the disease. Each year, from early April to October (excluding July and August), six spray treatments of 0.5% (v:v) Dentamet® were applied on the olive tree crowns. The compound reduced severity of symptoms in both cultivars. Most untreated trees died by the end of the trial, whereas all treated trees survived with good vegetative status as assessed by a normalized difference vegetation index. Quantitative real-time PCR was performed from June 2016 to September 2017, following the official procedures established by the European and Mediterranean Plant Protection Organization. The analysis revealed a statistically significant reduction of X. fastidiosa cell densities within the leaves of treated trees. These promising results suggest that integrated management to reduce severity of X. fastidiosa that includes regular pruning and soil harrowing with spring and summer spray treatments with Dentamet®, is likely to effectively control the disease.

 

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