Vol. 51 No. 1 (2012)
1st Special Issue on Mycotoxin risks in Mediterranean countries - Research pap.

Fusarium proliferatum isolated from garlic in Spain: identification, toxigenic potential and pathogenicity on related Allium species

Daniel PALMERO
Technical University of Madrid
Miguel DE CARA
University of Almería
Walid NOSIR
University of Aberdeen
Laura GÁLVEZ
Technical University of Madrid
Alejandra CRUZ
Department of Genetics, University Complutense of Madrid
Steve WOODWARD
University of Aberdeen
Maria GONZÁLEZ-JAÉN
Department of Genetics, University Complutense of Madrid
Julio TELLO
University of Almería

Published 2012-03-09

Keywords

  • fusaric acid,
  • fumonisin,
  • clove rot,
  • garlic

How to Cite

[1]
D. PALMERO, “Fusarium proliferatum isolated from garlic in Spain: identification, toxigenic potential and pathogenicity on related Allium species”, Phytopathol. Mediterr., vol. 51, no. 1, pp. 207–218, Mar. 2012.

Abstract

Fusarium proliferatum has been reported on garlic in the Northwest USA, Spain and Serbia, causing water-soaked tan-colored lesions on cloves. In this work, Fusarium proliferatum was isolated from 300 symptomatic garlic bulbs. Morphological identification of Fusarium was confirmed using species-specific PCR assays and EF-1α sequencing. Confirmation of pathogenicity was conducted with eighteen isolates. Six randomly selected F. proliferatum isolates from garlic were tested for specific pathogenicity and screened for fusaric acid production. Additionally, pathogenicity of each F. proliferatum isolate was tested on healthy seedlings of onion (Allium cepa), leek (A. porrum), scallions (A. fistulosum), chives (A. schoenoprasum) and garlic (A. sativum). A disease severity index (DSI) was calculated as the mean severity on three plants of each species with four test replicates. Symptoms on onion and garlic plants were observed three weeks after inoculation. All isolates tested produced symptoms on all varieties inoculated. Inoculation of F. proliferatum isolates from diseased garlic onto other Allium species provided new information on host range and pathogenicity. The results demonstrated differences in susceptibility with respect to host species and cultivar. The F. proliferatum isolates tested all produced fusaric acid (FA); correlations between FA production and isolate pathogenicity are discussed. Additionally, all isolates showed the presence of the FUM1 gene suggesting the ability of Spanish isolates to produce fumonisins.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...