Vol. 40, Supplement (2001) - 2nd IWGTD Special issue on Grapevine Trunk Diseases
Research Papers

Specific SCAR Primers for Fungi Associated with Wood Decay of Grapevine

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  • Angela Abbatecola,
  • Crescenza Dongiovanni,
  • Francesco Faretra,
  • Maria Antonietta De Guido,
  • Rita Milvia De Miccolis Angelini,
  • Stefania Pollastro
DOI: https://doi.org/10.14601/Phytopathol_Mediterr-1638

Published 2001-12-15

How to Cite

[1]
A. Abbatecola, C. Dongiovanni, F. Faretra, M. A. De Guido, R. M. De Miccolis Angelini, and S. Pollastro, “Specific SCAR Primers for Fungi Associated with Wood Decay of Grapevine”, Phytopathol. Mediterr., vol. 40, no. 4, pp. 362–368, Dec. 2001.
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Abstract

RAPD (Random Amplified Polymorphic DNA) analysis, a technique based on the polymerase chain reaction, was applied to explore variation in 178 isolates of Fomitiporia punctata, 94 of Phaeomoniella chlamydospora and 34 of Phomopsis viticola, selected as being representative of fungal populations from different vineyards and locations. The analysis showed a broad genetic variability in F. punctata and a very high genetic uniformity in P. chlamydospora. With P. viticola, isolates belonging to different vegetative compatibility groups were investigated; the analysis evidenced high genetic similarity among isolates within groups and broad inter-group variation. For each pathogen, specific RAPD markers were selected, cloned and sequenced. The obtained sequences were used to design sequence-characterised amplified region (SCAR) primers specific for each pathogen. These are being used to develop molecular diagnostic tools.
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