Vol. 61 No. 3 (2022): including the "60th MPU Anniversary Special Section"
60th MPU Anniversary Special Section - Review

Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review

Wanissa MELLIKECHE
Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, Via Napoli 25, 71122 Foggia
Giulia CASINI
Enbiotech S.r.l., Via Quarto dei Mille, 6, 90129 Palermo
Alessandra RICELLI
Istituto di Biologia e Patologia Molecolari (IBPM-CNR) P.le A. Moro 5, 00185, Roma
Giancarlo COLELLI
Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, Via Napoli 25, 71122 Foggia
Marilita GALLO
Centre International de Hautes Etudes Agronomiques Méditerranéennes Bari (CIHEAM Bari), Via Ceglie 9, 70010 Valenzano, Bari
Anna Maria D'ONGHIA
Centre International de Hautes Etudes Agronomiques Méditerranéennes Bari (CIHEAM Bari), Via Ceglie 9, 70010 Valenzano, Bari
Published January 13, 2023
Keywords
  • Food losses,
  • microorganism contamination,
  • mycotoxins,
  • LAMP
How to Cite
[1]
W. MELLIKECHE, G. CASINI, A. RICELLI, G. COLELLI, M. GALLO, and A. M. D’ONGHIA, “Detection of post-harvest pathogens by loop-mediated isothermal amplification: a review”, Phytopathol. Mediterr., vol. 61, no. 3, pp. 531-547, Jan. 2023.

Abstract

Postharvest losses, which occur between harvest and consumption of agricultural commodities, are major causes of food waste. Minimizing food loss helps provide nutritious food for animals and humans, and alleviate adverse environmental effects on food production. These losses are often related to the presence of postharvest pathogens, including fungi and bacteria, which typically start by infecting crops in the field as well as during postharvest chain. Control of these pathogens relies on development of tools that ensure their early and accurate detection. Among these is loop-mediated isothermal amplification (LAMP), a molecular method for pathogen detection. LAMP characteristics of rapidity, specificity and simplicity have encouraged development of a number of LAMP assays for detection of postharvest pathogens. Each LAMP assay allows to detect a specific genetic region of the target microorganism, which can be directly related to mycotoxin production, fungicide resistance and phytotoxicity. The LAMP amplicons are rapidly visualized, either at a specific timepoint, or in real-time by taking measurements throughout reaction, thereby necessitating less sophisticated facilities than those needed for PCR assays. In addition, many studies have developed simple protocols for the direct detection of pathogens on fresh produce. This paper explains the LAMP reaction, and its importance for postharvest detection of fungi and bacteria. Previous studies that have developed LAMP assays are also discussed.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...