Articles
Fusarium annulatum causes Fusarium rot of cantaloupe melons in Spain
María Ängeles PARRA- Juan Antonio MARTINEZ
Categories
Published 2022-07-19
Keywords
- Cucumis melo,
- fungal pathogen,
- pathogenicity test,
- first report,
- postharvest disease
How to Cite
[1]
M. Ängeles PARRA, J. GÓMEZ, F. W. AGUILAR, and J. A. MARTINEZ, “Fusarium annulatum causes Fusarium rot of cantaloupe melons in Spain”, Phytopathol. Mediterr., vol. 61, no. 2, pp. 269–277, Jul. 2022.
Funding data
-
Universidad Politécnica de Cartagena
Grant numbers Q8050013E
Abstract
During the summer of 2018, there was high incidence of fruit rots of cantaloupe melons (Cucumis melo var. cantalupensis) in Murcia province, south-eastern Spain. The fruits showed development of whitish mycelium and pulp softening. Morphological and molecular analysis of the internal transcribed spacer (ITS), translation elongation factor 1-α (TEF1-α) and the second largest subunit of RNA polymerase (RPB2) genes confirmed Fusarium annulatum as the causal agent of the disease. A phylogenetic study indicated that F. annulatum is in the Fusarium fujikuroi species complex (FFSC). Pathogenicity of the isolate was determined on healthy fruit verifying Koch’s postulates. The first symptoms of fruit rot were observed 3 d after inoculations at 28ºC. Fruit infections only occurred in artificially wounded melons, and F. annulatum was re-isolated from the wounds. This disease appeared after fruit harvesting, and could generate substantial economic losses mainly in fruit destined for foreign markets due to long transportation times. This is the first report of melon fruit rot caused by F. annulatum in Spain.
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References
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Chehri K., Salleh B., Yli-Mattila T., Reddy K.R.N., Abbasi S., 2011. Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah province, Iran. Saudi Journal of Biological Sciences 18: 341-351.
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O´Donnell K., Cigelnik E., Nirenberg H.I., 1998. Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90(3): 465-493.
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Promwee A., Yenjit P., Issarakraisila M., Intana W., Chamswarng C., 2017. Efficacy of indigeneous Trichoderma harzianum in controlling Phytophthora leaf fall (Phytophthora palmivora) in rubber trees. Journal of Plant Disease and Protection 124: 41-50.
Rivas-García T., Hernández-Montiel L.G., Amador B.M., Nieto-Garibay A., Chiquito-Contreras R.G., Rincón-Enríquez G., 2018. Identification and characterization of Fusarium spp. from muskmelon in northwest Mexico. Biotecnia 20(3): 71-75.
Snowdon A.L., 1991. A Colour Atlas of Post-harvest Diseases and Disorders of Fruits and Vegetables. Volume 2: Vegetables, Boca Raton: CRC Press, 416 pp.
Sung G.-H., Sung J.-M., Hywel-Jones N.L., Spatafora J.W., 2007. A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): Identification of localized incongruence using a combinational bootstrap approach. Molecular Phylogenetics and Evolution 44(3): 1204-1223.
White T.J., Bruns T., Lee S., Taylor J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: A Guide to Methods and Applications (M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White, ed.), Academic Press, San Diego, California, USA, 315-322.
Wigmann E.F., Behr J., Vogel R.F., Niessen L., 2019. MALDI-TOF MS fingerprinting for identification and differentiation of species within de Fusarium fujikuroi species complex. Applied Microbiology and Biotechnology 103: 5323-5337.
Wonglom P., Sunpapao A., 2020. Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo). Journal of Phytopathology 168: 204-210.
Yilmaz N., Sandoval-Denis M., Lombard L., Visagie C.M., Wingfield B.D., Crous P.W., 2021. Redefining species limits in the Fusarium fujikuroi species complex. Persoonia 46: 129-162.
Zitter T.A., Hopkins D.L., Thomas C.E., 1998. Compendium of cucurbit diseases, APS Press: St Paul, MN, USA, 87 pp.
Borrero C., Capote N., Gallardo M.A., Avilés M., 2019. First report of vascular wilt caused by Fusarium proliferatum on strawberry in Spain. Plant Disease 103(3): 581.
Bugnicourt F., 1952. Une espèce fusarienne nouvelle, parasite du riz. Revue Génerale de Botanique 59: 13-18 (in French).
Champaco E.R., Martyn R.D., Miller M.E., 1993. Comparison of Fusarium solani and F. oxysporum as causal agents of fruit rot and root rot of muskmelon. HortScience 28(12): 1174-1177.
Chehri K., Salleh B., Yli-Mattila T., Reddy K.R.N., Abbasi S., 2011. Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah province, Iran. Saudi Journal of Biological Sciences 18: 341-351.
Gálvez L., Urbaniak M., Waskiewicz A., Stepien L., Palmero D., 2017. Fusarium proliferatum – causal agent of garlic bulb rot in Spain: Genetic variability and mycotoxin production. Food Microbiology 67: 41-48.
Geiser D.M., Jiménez-Gasco M., Kang S., Makalowska I., Veeraraghavan N., … O´Donnell K., 2004. FUSARIUM-ID v. 1.0: A DNA sequence database for identifying Fusarium. European Journal of Plant Pathology 110(5-6): 473-479.
Gómez J., Guerra-Sanz J.M., Sánchez-Guerrero M.C., Serrano Y., Melero-Vara J.M., 2008. Crown rot of zucchini squash caused by Fusarium solani f. sp. cucurbitae in Almería Province, Spain. Plant Disease 92: 1137.
Gómez J., Serrano Y., Pérez A., Porcel E., Gómez R., Aguilar M.I., 2014. Fusarium solani f. sp. cucurbitae, affecting melon in Almería Province, Spain. Australasian Plant Disease Notes 9: 136.
Kim J.-W., Kim H.J., 2004. Fusarium fruit rot of postharvest oriental melon (Cucumis melo L. var. makuwa Mak.) caused by Fusarium spp. Research in Plant Disease 10(4): 260-267.
Leslie J.F., Summerell B.A., 2006. The Fusarium Laboratory Manual. Blackwell Professional, Ames, Iowa, USA, 388 pp.
Liu Y.J., Whelen S., Hall B.D., 1999. Phylogenetic relationship among ascomycetes: Evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16(12): 1799-1808.
MAPA, 2018. Ministerio de Agricultura, Pesca y Alimentación. Anuario de Estadística Agraria. Superficies y producciones de cultivos. Hortalizas de fruto-melón: Análisis provincial de superficie, rendimiento y producción. Available at: https://n9.cl/haga. Accessed June 06, 2021 (in Spanish).
Martínez R., Aguilar M.I., Guirado M.L., Álvarez A., Gómez J., 2003. First report of fusarium wilt of cucumber caused by Fusarium oxysporum in Spain. Plant Pathology 52: 410.
Martínez J.A., Jowkar M.M., Obando-Ulloa J.M., Varó P., Moreno E., Monforte A.J., Fernández-Trujillo J.P., 2009. Uncommon disorders and decay in near-isogenic lines of melon and reference cultivars. Horticultura Brasileira 27:505-514.
Nelson P.E., Toussoun T.A., Marasas W.F.O., 1983. Fusarium species: An illustrated manual for identification. Pennsylvania State University Press, University Park, 193 pp.
Nelson P.E., Plattner R.D., Shackelford D.D., Desjardins A.E., 1992. Fumonisin B1 production by Fusarium species other than F. moniliforme in section Liseola and by some related species. Applied and Environmental Microbiology 58(3): 984-989.
Nuangmek W., Aiduang W., Suwannarach N., Kumla J., Kiatsiriroat T., Lumyong S., 2019. First report of fruit rot on cantaloupe caused by Fusarium equiseti in Thailand. Journal of General Plant Pathology 85: 295-300.
O´Donnell K., Cigelnik E., Nirenberg H.I., 1998. Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90(3): 465-493.
O´Donnell K., Sutton D.A., Rinaldi M.G., Sarver B.A.J., Balajee S.A., … Geiser D.M., 2010. Internet-accessible DNA sequence database for identifying Fusaria from human and animal infections. Journal of Clinical Microbiology 48(10): 3708-3718.
Promwee A., Yenjit P., Issarakraisila M., Intana W., Chamswarng C., 2017. Efficacy of indigeneous Trichoderma harzianum in controlling Phytophthora leaf fall (Phytophthora palmivora) in rubber trees. Journal of Plant Disease and Protection 124: 41-50.
Rivas-García T., Hernández-Montiel L.G., Amador B.M., Nieto-Garibay A., Chiquito-Contreras R.G., Rincón-Enríquez G., 2018. Identification and characterization of Fusarium spp. from muskmelon in northwest Mexico. Biotecnia 20(3): 71-75.
Snowdon A.L., 1991. A Colour Atlas of Post-harvest Diseases and Disorders of Fruits and Vegetables. Volume 2: Vegetables, Boca Raton: CRC Press, 416 pp.
Sung G.-H., Sung J.-M., Hywel-Jones N.L., Spatafora J.W., 2007. A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): Identification of localized incongruence using a combinational bootstrap approach. Molecular Phylogenetics and Evolution 44(3): 1204-1223.
White T.J., Bruns T., Lee S., Taylor J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: A Guide to Methods and Applications (M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White, ed.), Academic Press, San Diego, California, USA, 315-322.
Wigmann E.F., Behr J., Vogel R.F., Niessen L., 2019. MALDI-TOF MS fingerprinting for identification and differentiation of species within de Fusarium fujikuroi species complex. Applied Microbiology and Biotechnology 103: 5323-5337.
Wonglom P., Sunpapao A., 2020. Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo). Journal of Phytopathology 168: 204-210.
Yilmaz N., Sandoval-Denis M., Lombard L., Visagie C.M., Wingfield B.D., Crous P.W., 2021. Redefining species limits in the Fusarium fujikuroi species complex. Persoonia 46: 129-162.
Zitter T.A., Hopkins D.L., Thomas C.E., 1998. Compendium of cucurbit diseases, APS Press: St Paul, MN, USA, 87 pp.