Short Notes
Characterization of a Syrian Chickpea chlorotic stunt virus strain and production of polyclonal antibodies for its detection
Published 2012-11-12
Keywords
- CpCSV,
- purification
How to Cite
[1]
Y. ALNAASAN, S. KUMARI, J. VAN LEUR, A. HAJ KASSEM, and F. AZMEH, “Characterization of a Syrian Chickpea chlorotic stunt virus strain and production of polyclonal antibodies for its detection”, Phytopathol. Mediterr., vol. 52, no. 1, pp. 130–135, Nov. 2012.
Copyright (c) 2012 Yaseen ALNAASAN, Safaa KUMARI, Joop VAN LEUR, Amin HAJ KASSEM, Fawaz AZMEH
This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
Reverse transcription-polymerase chain reaction analysis with two primer sets of luteoviruses was used to characterize an isolate of Chickpea chlorotic stunt virus (CpCSv, genus Polerovirus, family Luteoviridae) (SC402-08) collected from Lattakia, Syria, during the 2007‒2008 chickpea growing season. Sequence analysis revealed that the coat protein gene of the isolate shared nucleotide sequence identities ranging from 97 to 98% with the CpCSv isolates from Egypt, morocco and Syria. The capsid protein was separated as a protein of approximately 20 kDa in sodium dodecyl sulphate polyacrylamide gel electrophoresis, and was visually detected by its reaction with CpCSV monoclonal antibody in Western blot. SC402-08 isolate of CpCSV was purified from faba bean-infected plants, and yielded 112–182 μg of purified virions kg-1 of infected tissue. The purified preparation was injected into a white rabbit, and an antiserum was obtained and used to detect CpCSv in infected tissues by tissue-blot immunoassay. The antiserum obtained was able to detect CpCSv by the immunoassay up to a dilution of 1:1,024,000.Downloads
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