Vol. 64 No. 1 (2025)
Articles

Pea seed-borne mosaic virus pathotypes isolated from Australian pea (Pisum sativum) seed

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  • Joop VAN LEUR,
  • Mohammad AFTAB,
  • Angela FREEMAN
Joop VAN LEUR
New South Wales Department of Primary Industries and Regional Development, Tamworth Agricultural Institute, Tamworth, Australia
Mohammad AFTAB
Agriculture Victoria, The Grains Innovation Park, Horsham, Australia
Angela FREEMAN
Agriculture Victoria, The Grains Innovation Park, Horsham, Australia
DOI: https://doi.org/10.36253/phyto-15934

Published 2025-05-14

Keywords

  • PSbMV,
  • BYMV,
  • virus resistance,
  • pathogenicity test

How to Cite

[1]
J. VAN LEUR, M. AFTAB, and A. FREEMAN, “Pea seed-borne mosaic virus pathotypes isolated from Australian pea (Pisum sativum) seed”, Phytopathol. Mediterr., vol. 64, no. 1, pp. 71–76, May 2025.

Copyright (c) 2025 Joop VAN LEUR, Mohammad AFTAB, Angela FREEMAN

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Pea seed lots (144) from Australian farms and research trials were assessed for pea seed-borne mosaic virus (PSbMV) seed transmission rates. High infection rates (up to 40%) were detected, particularly in the widely grown and highly PSbMV susceptible variety ‘Kaspa’, with only 12 out of 54 seed lots found to be free of the virus. PSbMV strains were isolated from 15 infected seed lots, and were pathotyped on a set of homogeneous Pisum sativum PSbMV differentials. Of the four pathotypes identified, P1 and P4 (eight and 20 isolates, respectively) were earlier reported in Australia. Pathotype P3, as yet unreported in Australia, was the most frequently identified pathotype (42 isolates). One PSbMV isolate was identified as the P2 pathotype, which has previously been isolated only from lentil seed. None of the isolated pathotypes could overcome the PSbMV resistance gene sbm1. The relevance of these findings for field pea breeding programmes and genomic studies of PSbMV are discussed.

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References

  1. Alconero R., Provvidenti R., Gonsalves D., 1986. Three pea seedborne mosaic virus pathotypes from pea and lentil germ plasm. Plant Disease 70: 783–786. https://doi.org/10.1094/PD-70-783 DOI: https://doi.org/10.1094/PD-70-783
  2. Ashby J.W., Fletcher J.D., Jermyn W.A., Goulden D., 1986. Some properties of a strain of pea seed-borne mosaic virus isolated from field peas in New Zealand. New Zealand Journal of Experimental Agriculture 14: 209–213. https://doi.org/10.1080/03015521.1986.10426145 DOI: https://doi.org/10.1080/03015521.1986.10426145
  3. Congdon B.S., Coutts B.A., Renton M., Banovic M., Jones R.A.C., 2016. Pea seed-borne mosaic virus in field pea: widespread infection, genetic diversity, and resistance gene effectiveness. Plant Disease 100: 2475–2482. https://doi.org/10.1094/PDIS-05-16-0670-RE DOI: https://doi.org/10.1094/PDIS-05-16-0670-RE
  4. Congdon B.S., 2017. Understanding, Forecasting and Managing Pea Seed-borne Mosaic Virus in Field Pea. PhD Thesis, The University of Western Australia. https://research-repository.uwa.edu.au/en/publications/understanding-forecasting-and-managing-pea-seed-borne-mosaic-viru. (accessed 03-01-25).
  5. Coutts B.A., Prince R.T., Jones R.A.C., 2009. Quantifying effects of seedborne inoculum on virus spread, yield losses, and seed infection in the Pea seed-borne mosaic virus - field pea pathosystem. Phytopathology 99: 1156–1167. https://doi.org/10.1094/PHYTO-99-10-1156 DOI: https://doi.org/10.1094/PHYTO-99-10-1156
  6. Fletcher J.D., 1993 Surveys of virus diseases in pea, lentil, dwarf and broad bean crops in South Island, New Zealand. New Zealand Journal of Crop and Horticultural Science 21: 45–52. https://doi.org/10.1080/01140671.1993.9513745 DOI: https://doi.org/10.1080/01140671.1993.9513745
  7. Freeman A., Spackman M., Aftab M., McQueen V., King S., … Rodoni B., 2013. Comparison of Tissue blot immunoassay and high throughput PCR for virus-testing samples from a southeastern Australian pulse virus survey. Australasian Plant Pathology 42: 675–683. https://doi.org/10.1007/s13313-013-0252-9 DOI: https://doi.org/10.1007/s13313-013-0252-9
  8. Gao Z., Johansen I.E., Eyers S., Thomas C.L., Ellis T.H.N., Maule A.J., 2004. The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking. The Plant Journal 40: 376–385. https://doi.org/10.1111/j.1365-313X.2004.02215.x DOI: https://doi.org/10.1111/j.1365-313X.2004.02215.x
  9. Hampton R.O., 1982. Incidence of the lentil strain of Pea seedborne mosaic virus as a contaminant of Lens culinaris germ plasm. Phytopathology 72: 695–698. https://doi.org/10.1094/Phyto-72-695 DOI: https://doi.org/10.1094/Phyto-77-695
  10. Hjulsager C.K., Lund O.S., Johansen I.E., 2002. A new pathotype of Pea seedborne mosaic virus explained by properties on the P3-6k1- and viral genome-linked protein (VPg) - coding regions. Molecular Plant-Microbe Interactions 15: 169–171. https://doi.org/10.1094/MPMI.2002.15.2.169. DOI: https://doi.org/10.1094/MPMI.2002.15.2.169
  11. Johansen I.E., Lund O.S., Hjulsager C.K., Laursen J., 2001. Recessive resistance in Pisum sativum and potyvirus pathotype resolved in a gene-for-cistron correspondence between host and virus. Journal of Virology 75: 6609–6614. https://doi.org/10.1128/JVI.75.14.6609–6614.2001 DOI: https://doi.org/10.1128/JVI.75.14.6609-6614.2001
  12. Khetarpal R.K., Maury Y., 1987. Pea seed-borne mosaic virus: a review. Agronomie 7: 215–224. https://doi.org/10.1051/agro:19870401 DOI: https://doi.org/10.1051/agro:19870401
  13. Kumari, S.G., Moukahel A., El Miziani I, 2022. Diagnostic tools validated by ICARDA’s Germplasm Health Unit (GHU) for detection of legume seed-borne pests. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon. https://hdl.handle.net/10568/126879 (accessed 03-01-25).
  14. Latham L.J., Jones R.A.C., 2001a. Alfalfa mosaic and pea seed-borne mosaic viruses in cool season crop, annual pasture, and forage legumes: susceptibility, sensitivity and seed transmission. Australian Journal of Agricultural Research 52: 771–790. https://doi.org/10.1071/AR00165 DOI: https://doi.org/10.1071/AR00165
  15. Latham L.J., Jones R.A.C., 2001b. Incidence of virus infection in experimental plots, commercial crops, and seed stocks of cool season crop legumes. Australian Journal of Agricultural Research 52: 397-413. https://doi.org/10.1071/AR00079 DOI: https://doi.org/10.1071/AR00079
  16. Ligat J.S., Cartwright D., Randles J.W., 1991. Comparison of some pea seed-borne mosaic virus isolates and their detection by dot-immunobinding assay. Australian Journal of Agricultural Research 42: 441–451. https://doi.org/10.1071/AR9910441 DOI: https://doi.org/10.1071/AR9910441
  17. Ligat J.S., Randles J.W., 1993. An eclipse of pea seed-borne mosaic virus in vegetative tissue of pea following repeated transmission through the seed. Annals of Applied Biology 122: 39–47. https://doi.org/10.1111/j.1744-7348.1993.tb04012.x DOI: https://doi.org/10.1111/j.1744-7348.1993.tb04012.x
  18. Provvidenti R., Alconero R., 1988. Inheritance of resistance to a third pathotype of pea seed-borne mosaic virus in Pisum sativum. Journal of Heredity 79: 76–77. https://doi.org/10.1093/oxfordjournals.jhered.a110457 DOI: https://doi.org/10.1093/oxfordjournals.jhered.a110457
  19. Rosewarne G., 2016. DAV00118 - Pulse Breeding Australia: Field Pea Breeding Program. https://grdc.com.au/research/reports/report?id=6831 (accessed 03-01-25).
  20. Safarova D., Navratil M., Petrusova J., Pokorny R., Piakova Z., 2008. Genetic and biological diversity of the pea seed-borne mosaic virus isolates occurring in Czech Republic. Acta Virologica 52: 53–57.
  21. Siddique K.H.M., Sykes J., 1997. Pulse production in Australia past, present and future. Australian Journal of Experimental Agriculture 37: 103–111. https://doi.org/10.1071/EA96068 DOI: https://doi.org/10.1071/EA96068
  22. Torok V.A., Randles J.W., 2007. Discriminating between isolates of PSbMV using nucleotide sequence polymorphisms in the HC-Pro coding region. Plant Disease 91: 490–496. https://doi.org/10.1094/PDIS-91-5-0490 DOI: https://doi.org/10.1094/PDIS-91-5-0490
  23. van Leur J.A.G., Kumari S.G., Aftab M., Leonforte A., Moore S., 2013a. Virus resistance of Australian pea (Pisum sativum) varieties. New Zealand Journal of Crop & Horticultural Science 41: 86–101. https://doi.org/10.1080/01140671.2013.781039 DOI: https://doi.org/10.1080/01140671.2013.781039
  24. van Leur J.A.G., Freeman A., Aftab M., Spackman M., Redden B., Materne M., 2013b. Identification of seed-borne Pea seed-borne mosaic virus in lentil (Lens culinaris) germplasm and strategies to avoid its introduction in commercial Australian lentil fields. Australasian Plant Disease Notes 8: 75–77. https://doi.org/10.1007/s13314-013-0099-5 DOI: https://doi.org/10.1007/s13314-013-0099-5
  25. Wylie S.J., Couts B.A., Jones R.A.C., 2011. Genetic variability of the coat protein sequence of pea seed-borne mosaic virus isolates and the current relationship between phylogenetic placement and resistance groups. Archive of Virology 156: 1287–1290. https://doi.org/10.1007/s00705-011-1002-3 DOI: https://doi.org/10.1007/s00705-011-1002-3