Vol. 54 No. 1 (2015)
Research Papers

<em>Fusarium oxysporum</em> f. sp. <em>papaveris</em>: a new forma specialis isolated from iceland poppy (<em>Papaver nudicaule</em>)

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  • Giuseppe ORTU,
  • Domenico BERTETTI,
  • Patrizia MARTINI,
  • Maria Lodovica GULLINO,
  • Angelo GARIBALDI
Giuseppe ORTU
Agroinnova – Centre of Competence for the Innovation in the Agro-Environmental Sector, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Turin, Italy
Domenico BERTETTI
Agroinnova – Centre of Competence for the Innovation in the Agro-Environmental Sector, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Turin, Italy.
Patrizia MARTINI
Istituto Regionale per la Floricoltura, Via Carducci 12, 18038 Sanremo, Italy
Maria Lodovica GULLINO
Agroinnova – Centre of Competence for the Innovation in the Agro-Environmental Sector, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Turin, Italy DISAFA - Dipartimento di Scienze Agrarie, Forestali e Alimentari, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Turin, Italy.
Angelo GARIBALDI
Agroinnova – Centre of Competence for the Innovation in the Agro-Environmental Sector, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Turin, Italy.
DOI: https://doi.org/10.14601/Phytopathol_Mediterr-14303

Published 2015-04-12

Keywords

  • Fusarium,
  • EF-1α,
  • endopolygalacturonase

How to Cite

[1]
G. ORTU, D. BERTETTI, P. MARTINI, M. L. GULLINO, and A. GARIBALDI, “<em>Fusarium oxysporum</em> f. sp. <em>papaveris</em>: a new forma specialis isolated from iceland poppy (<em>Papaver nudicaule</em>)”, Phytopathol. Mediterr., vol. 54, no. 1, pp. 76–85, Apr. 2015.
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Abstract

Symptoms of a new vascular wilt were observed on several plants of Papaver nudicaule cultivated at the Regional Institute for Floriculture in Sanremo and in a nursery near Ventimiglia, Liguria region, Northern Italy. Morphology and ITS sequence analysis characterize the pathogen as Fusarium oxysporum. To identify the forma specialis, eight isolates obtained from infected tissues were used for a phylogenetic analysis based on translation elongation factor 1-α (EF-1α) gene and four polygalacturonase genes (Pg1, Pg5, Pgx1 and Pgx4). After amplification by PCR, the gene sequences were aligned with corresponding sequences from other formae speciales of Fusarium oxysporum obtained from GenBank databases in order to build phylogenetic trees. Results obtained for each genomic region showed that the isolates derived from P. nudicaule form a unique group well separated from other formae speciales. To our knowledge, this is the first report of F. oxysporum on P. nudicaule and together with the phylogenetic analysis and positive pathogenicity tests permits us to introduce a new forma specialis named F. oxysporum f. sp. papaveris.

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