Vol. 50 No. 2 (2011)
Research Papers

Genotypic Diversity of Iranian strains of Ralstonia solanacearum

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  • Mahsa IZADIYAN,
  • S. Mohsen TAGHAVI
S. Mohsen TAGHAVI
Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran
DOI: https://doi.org/10.14601/Phytopathol_Mediterr-9046

Published 2011-07-11

Keywords

  • bacterial wilt,
  • Iranian biovars

How to Cite

[1]
M. IZADIYAN and S. M. TAGHAVI, “Genotypic Diversity of Iranian strains of Ralstonia solanacearum”, Phytopathol. Mediterr., vol. 50, no. 2, pp. 236–244, Jul. 2011.

Copyright (c) 2011 Mahsa IZADIYAN, S. Mohsen TAGHAVI

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

During 2007–2009, 170 bacterial isolates were obtained from wilting potato and tomato plants from Iran. Specific polymerase chain reaction (PCR) analyses identified all the isolates as Ralstonia solanacearum. Diversity among the Iranian isolates was determined by a study based on biovar determination, pathogenicity, repetitive sequence PCR and phylotype classification. On the basis of  genotypic, phenotypic and pathogenic characteristics two distinct clusters obtained. Cluster I comprised all biovar 2 isolates and cluster II comprised all biovar N2 (2T) isolates. Phylotype-specifi c multiplex PCR (pmx-PCR) indicated that all biovar 2 and N2 belong to phylotype II. This is the first report of phylotype determination of biovars of R. solanacearum with pmx-PCR in Iran.

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