Vol. 64 No. 1 (2025)
Articles

Microbiota dynamic communities in sweet orange infected by “huanglongbing” in Iran: Microbiota Associated with Sweet Orange Infected by Huanglongbing in Iran

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  • Shiva SAFARPOUR KAPOURCHALI,
  • Mojdeh MALEKI,
  • Ali ALIZADEH ALIABADI,
  • Saeideh RAJAEE,
  • Mohammad MEHDI FAGHIHI,
  • Mehdi NASR ESFAHANI
Shiva SAFARPOUR KAPOURCHALI
Department of Plant Protection, Islamic Azad University, Varamin, Pishva Branch, Varamin, Iran
Mojdeh MALEKI
Department of Plant Pathology, Islamic Azad University, Varamin, Pishva Branch, Varamin, Iran
Ali ALIZADEH ALIABADI
Iranian Research Institute of Plant Protection, AREEO, Tehran, Iran
Saeideh RAJAEE
The National Institute of Genetic Engineering and Biotechnology, NIGEB, Tehran, Iran
Mohammad MEHDI FAGHIHI
Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Zarghan, Iran
Mehdi NASR ESFAHANI
Plant Protection Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran
DOI: https://doi.org/10.36253/phyto-15662
Categories

Published 2025-05-14

Keywords

  • ‘Candidatus Liberibacter’ species,
  • plant microbiota,
  • Valencia sweet orange

How to Cite

[1]
S. SAFARPOUR KAPOURCHALI, M. MALEKI, A. ALIZADEH ALIABADI, S. RAJAEE, M. MEHDI FAGHIHI, and M. NASR ESFAHANI, “Microbiota dynamic communities in sweet orange infected by ‘huanglongbing’ in Iran: Microbiota Associated with Sweet Orange Infected by Huanglongbing in Iran”, Phytopathol. Mediterr., vol. 64, no. 1, pp. 129–143, May 2025.

Copyright (c) 2025 Shiva SAFARPOUR KAPOURCHALI, Mojdeh MALEKI, Ali ALIZADEH ALIABADI, Saeideh RAJAEE, Mohammad MEHDI FAGHIHI, Mehdi NASR ESFAHANI

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

“Huanglongbing”-(HLB) or citrus-greening is one of the most serious citrus diseases worldwide. This study aimed to investigate the bacterial-communities associated with HLB-symptomatic sweet orange trees (Citrus sinensis) from different geographical regions in southern Iran. The 16S rRNA gene amplicon metagenomics sequencing of DNA extracted from the midrib and petiole tissues of symptomatic plants confirmed that the ‘Candidatus Liberibacter asiaticus’, was spread along the citrus-plantation regions in southern Iran, including Kerman, Sistan and Baluchistan, Fars, Hormozgan, and Khuzestan Provinces. The frequency of Operational Taxonomic Units (OTUs) related to ‘Ca. L. asiaticus’ was remarkable in the HLB symptomatic tree in the Fars region. No OTUs of ‘Ca. Liberibacter’ or ‘Ca. Phytoplasma’ were detected in the asymptomatic samples in the Kerman region. However, in asymptomatic materials representatives of the class Bacilli, including Lactobacillus spp. and Bacillus spp., showed 12- and 4-fold presence compared to the symptomatic samples of Kerman groves. Furthermore, the presence of OTUs belonging to ‘Ca. L. europaeus’ and ‘Ca. Phytoplasma aurantifolia’ was detected in sweet oranges. The simultaneous occurrence of ‘Ca. L. asiaticus’, ‘Ca. L. europaeus’, and ‘Ca. P. aurantifolia’ in HLB symptomatic orange trees in the Fars groves provided worthwhile insights for further research, although their epidemiological role in co-infections remains unknown. Microbial dataset in relation to variables associated with the plant health, defense, and disease helps to understand how these variables shape the citrus microbial community and identify individual that play a role in HLB suppression or promotion.

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