Vol. 63 No. 2 (2024)
Articles

Characterization and genetic diversity of grapevine Pinot gris virus in Serbian vineyards

Sanja ŽIVKOVIĆ
University of Niš, Faculty of Agriculture, Kosančićeva 4, 37000 Kruševac
Bojana VASILIJEVIĆ
Fruit Research Institute, Kralja Petra I 9, 32102 Čačak
Bio
Tanja VASIĆ
University of Niš, Faculty of Agriculture, Kosančićeva 4, 37000 Kruševac
Bio
Debasis MITRA
Department of Microbiology, Graphic Era (Deemed to be University), 566/6 Bell Road, Clement Town, Dehradun, 248002 Uttarakhand, India
Bio
Darko JEVREMOVIĆ
Fruit Research Institute, Kralja Petra I 9, 32102 Čačak
Bio
Categories

Published 2024-09-16

Keywords

  • Vitis vinifera L.,
  • RT-PCR,
  • detection,
  • phylogenetic analyses

How to Cite

[1]
ŽIVKOVIĆ S., VASILIJEVIĆ B., VASIĆ T., D. MITRA, and JEVREMOVIĆ D., “Characterization and genetic diversity of grapevine Pinot gris virus in Serbian vineyards”, Phytopathol. Mediterr., vol. 63, no. 2, pp. 315–321, Sep. 2024.

Abstract

Sixty-five samples of grapevine from commercial vineyards in the Rasina district of Serbia were tested for the presence of grapevine Pinot gris virus (GPGV), using the reverse transcription-polymerase chain reaction. Fourteen samples of the grapevine varieties ‘Red Globe’, ‘Victoria’ and ‘Preobraženje’ were infected with GPGV. All the infected plants showed symptoms of leaf chlorotic mottling, puckering, and deformation, stunting, and reduced yields. The coding regions of the movement and coat protein (MP/CP) and a region of the RNA-dependent RNA polymerase (RdRP) domains of eight virus isolates were sequenced. Phylogenetic analyses of these genomic regions showed high nucleotide similarity among the Serbian GPGV isolates. This study is the first to describe genetic diversity of GPGV isolates in Serbia.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

  1. Angelini E, Bazzo I., Bertazzon N., Filippin L., Forte V., 2015. A new disease in the vineyard: Grapevine. leaf mottling and deformation. The Australian & New Zealand Grapegrower and Winemaker 622: 56–59.
  2. Bertazzon N., Filippin L., Forte V., Angelini E., 2016. Grapevine Pinot gris virus seems to have recently been introduced to vineyards in Veneto, Italy. Archives of Virology 161(3): 711–714. https://doi.org/10.1007/s00705-015-2718-2 DOI: https://doi.org/10.1007/s00705-015-2718-2
  3. Bertazzon N., Forte V., Filippin L., Causin R., Maixner M., Angelini E., 2017. Association between genetic variability and titre of Grapevine Pinot gris virus with disease symptoms. Plant Pathology 66: 949–959. doi: 10.1111/ppa.12639 DOI: https://doi.org/10.1111/ppa.12639
  4. Bertazzon N., Forte V., Angelini E., 2020. Fast transmission of grapevine ‘Pinot gris’ virus (GPGV) in vineyard. Vitis 59: 29–34. https://doi.org/10.5073/vitis.2020.59.29-34
  5. Eichmeier A., Peňázová E., Pavelková R., Mynarzová Z., Saldarelli P., 2016a. Detection of grapevine pinot gris virus in certified grapevine stocks in Moravia, Czech Republic. Journal of Plant Pathology 98: 155–157. https://doi.org/10.4454/JPP.V98I1.043
  6. Eichmeier A., Komínková M., Komínek P., Baránek M., 2016b. Comprehensive virus detection using next generation sequencing in grapevine vascular tissues of plants obtained from the wine regions of bohemia and Moravia (Czech Republic). PloS One 11, e0167966. https://doi.org/10.1371/journal.pone.0167966. DOI: https://doi.org/10.1371/journal.pone.0167966
  7. Eichmeier A., Pieczonka K., Peňázová E., Pečenka J., Gajewski Z., 2017. Occurrence of grapevine pinot gris virus in Poland and description of asymptomatic exhibitions in grapevines. Journal of Plant Diseases and Protection 124: 407–411. https://doi.org/10.1007/s41348-017-0076-x DOI: https://doi.org/10.1007/s41348-017-0076-x
  8. EPPO, 2024. GPGV distribution. Available at: https://gd.eppo.int/taxon/GPGV00/distribution. Retrieved 10 May 2024.
  9. Fuchs, M., 2020. Grapevine viruses: a multitude of diverse species with simple but overall poorly adopted management solutions in the vineyard. Journal of Plant Pathology 102: 643–653. https://doi.org/10.1007/s42161-020-00579-2 DOI: https://doi.org/10.1007/s42161-020-00579-2
  10. Gambino G., Gribaudo I., 2006. Simultaneous detection of nine grapevine viruses by multiplex reverse transcription-polymerase chain reaction with coamplification of a plant RNA as internal control. Phytopathology 96(11): 1223–1229. https://doi.org/10.1094/PHYTO-96-1223 DOI: https://doi.org/10.1094/PHYTO-96-1223
  11. Gentili A., Prota V., Moro G., Schianchi N., Di Lucca E., Luigi M., Faggioli F., 2017. Identification of grapevine ‘Pinot gris’ virus in Sardinia and Lazio (South and Central Italy). Journal of Plant Pathology 99: 527–535. https://doi.org/10.4454/JPP.V99I2.3872
  12. Giampetruzzi A., Roumi V, Roberto R, Malossini U, Yoshikawa N, … Saldarelli P. 2012. A new grapevine virus discovered by deep sequencing of virus– And viroid–derived small RNAs in cv pinot gris. Virus Research 163: 262–268. https://doi.org/10.1016/j.virusres.2011.10.010 DOI: https://doi.org/10.1016/j.virusres.2011.10.010
  13. Hall T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid Symposium Series 41: 95–98.
  14. Hily J.M., Poulicard N., Candresse T., Vigne E., Beuve M., … Lemaire O., 2020. Datamining, genetic diversity analyses, and phylogeographic reconstructions redefine the worldwide evolutionary history of grapevine pinot gris virus and grapevine berry inner necrosis virus. Phytobiomes Journal 4(2): 165–177. https://doi.org/10.1094/PBIOMES-10-19-0061-R DOI: https://doi.org/10.1094/PBIOMES-10-19-0061-R
  15. Li R., Mock R., Huang Q., Abad J., Hartung J., Kinard G., 2008. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. Journal of Virological Methods 154: 48–55. https://doi.org/10.1016/j.jviromet.2008.09.008 DOI: https://doi.org/10.1016/j.jviromet.2008.09.008
  16. Malagnini V., de Lillo E., Saldarelli P., Beber R., Duso C., … Gualandri V., 2016. Transmission of grapevine Pinot gris virus by Colomerus vitis (Acari: Eriophyidae) to grapevine. Archives of Virology 161(9): 2595-2599. https://doi.org/10.1007/s00705-016-2935-3 DOI: https://doi.org/10.1007/s00705-016-2935-3
  17. Malossini U., Moscon R., Ferrazza M., Bianchedi P., Varner M., Credi R., 2012. Caratteristiche vegeto-produttive di viti Pinot grigio e Traminer aromatico affette da una nuova virosi segnalata in Trentino [Vegetative-productive characteristics of Pinot grigio and Traminer aromatico vines affected by a new virus reported in Trentino]. In: Proceedings IV Convegno Nazionale di Viticoltura: CONAVI.TO, 10–12 July, 2012, Asti, Italy, 37 (abstract).
  18. Martelli G.P., 2014. Directory of virus and virus-like diseases of the grapevine and their agents. Journal of Plant Pathology 96: 105–120. https://doi.org/10.4454/JPP.V96I1SUP DOI: https://doi.org/10.1002/9780470015902.a0000766.pub3
  19. Saldarelli P., Beber R., Novelli L., Bianchedi P., Credi R., … Gualandri V., 2013. Studies on a new grapevine disease in Trentino vineyards. Journal of Plant Pathology 95: S4.60
  20. Saldarelli P., Giampetruzzi A., Morelli M., Malossini U., Pirolo C., Bianchedi P., Gualandri V., 2015. Genetic variability of Grapevine Pinot gris virus and its association with grapevine leaf mottling and deformation. Phytopathology 105: 555–563. https://doi.org/10.1094/PHYTO-09-14-0241-R DOI: https://doi.org/10.1094/PHYTO-09-14-0241-R
  21. Tamura K., Stecher G., Kumar S., 2021. MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution 38: 3022–3027. https://doi.org/10.1093/molbev/msab120 DOI: https://doi.org/10.1093/molbev/msab120
  22. Tarquini G., Ermacora P., Bianchi G.L., De Amicis F., Pagliari L., … Musetti R., 2018. Localization and subcellular association of Grapevine Pinot Gris Virus in grapevine leaf tissues. Protoplasma 255(3): 923–935. https://doi.org/10.1007/s00709-017-1198-5 DOI: https://doi.org/10.1007/s00709-017-1198-5