Vol. 63 No. 2 (2024)
Articles

Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China

Wen LI
Ningbo City College of Vocational Technology, Ningbo Zhejiang
Yixuan RUAN
Ningbo City College of Vocational Technology, Ningbo Zhejiang
Zhengping BIAN
Ningbo Forest Farm, Ningbo Zhejiang
Yueqiu HE
Ningbo City College of Vocational Technology, Ningbo Zhejiang
Kai FENG
Ningbo City College of Vocational Technology, Ningbo Zhejiang
Liang LIU
Ningbo City College of Vocational Technology, Ningbo Zhejiang
Zhilong WANG
Ningbo City College of Vocational Technology, Ningbo Zhejiang
Feng HUANG
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High, Guangzhou, Guangdong
Categories

Published 2024-09-16

Keywords

  • Chemical control,
  • new disease,
  • pathogen identification,
  • phylogeny

How to Cite

[1]
W. LI, “Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China”, Phytopathol. Mediterr., vol. 63, no. 2, pp. 295–301, Sep. 2024.

Abstract

Sweet viburnum (Viburnum odoratissimum Ker-Gawl.) is a widely used ornamental plant, which has dense branches and leaves, and fast spreading and evergreen habit. In October 2022, leaf spot symptoms were observed in a hedge of sweet viburnum in Yuanshi Garden, Ningbo, China. Fungi were isolated from symptomatic leaves, and were identified using morphological characteristics and phylogenetic analyses of partial sequences of internal transcribed spacer (ITS), actin (act), and translation elongation factor 1-alpha (tef1-α), and were evaluated in pathogenicity tests. The causal agent of sweet viburnum leaf spot was identified to be Phyllosticta capitalensis. Effects of seven fungicides on P. capitalensis were assessed in vitro. Fungicide EC50s (mg L-1) against P. capitalensis were: 270.77 for 75% chlorothalonil (WP); 0.02 for 250 g L-1 azoxystrobin SC; 0.27 for 10% difenconazole WDG; 0.02 for 75% trifloxystrobin + tebuconazole WDG, 9.03 for 35% fluopyram + tebuconazole SC, 5.90 for 500 g L-1 fluazinam SC, and 89.11 for 10% prothioconazole SC. Among these, azoxystrobin SC and trifloxystrobin + tebuconazole WDG could be used for control of viburnum leaf spot. This is the first report of P. capitalensis causing leaf spot of sweet viburnum, and this study provides guidance for chemical management sweet viburnum leaf spot, and on other host plants.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

  1. Bogas A.C., Cruz F.P.N., Lacava P.T., Sousa C.P., 2022. Endophytic fungi: an overview on biotechnological and agronomic potential. Brazilian Journal of Biology 84: e258557. https://doi.org/10.1590/1519-6984.258557 DOI: https://doi.org/10.1590/1519-6984.258557
  2. Cho S.E., Lee S.H., Lee S.Y., Lee C.K., Shin H.D., 2016. First report of powdery mildew caused by Erysiphe hedwigii on Viburnum awabuki in Korea. Plant Disease 100: 2533. https://doi.org/10.1094/PDIS-06-16-0836-PDN DOI: https://doi.org/10.1094/PDIS-06-16-0836-PDN
  3. Cui X.Y., Dan Y.R., Li J.J., Ma W.L., Tang Z.X., … Liu M. 2023. Identification, biological characterization and inhibitory effect of fungicides on the pathogen causing Polygonatum cyrtonema leaf spot. Acta Phytopathologica Sinica 53(6): 1038-1046.
  4. Damm U., Woudenberg J.H.C., Cannon P.F., Crous P.W., 2009. Colletotrichum species with curved conidia from herbaceous hosts. Fungal Diversity 39: 1–119. https://doi.org/10.1016/j.riam.2009.11.001 DOI: https://doi.org/10.1016/j.riam.2009.11.001
  5. Gao Y.W., Xu Z.T., Li P., Hu X.D., Chen J.P., … Li Y.Y., 2024. Complete genome analysis of a novel narnavirus in sweet viburnum (Viburnum odoratissimum). Archives of Virology 169: 90. https://doi.org/10.1007/s00705-024-06000-y DOI: https://doi.org/10.1007/s00705-024-06000-y
  6. Glienke-Blanco C., Aguilar-Vildoso C.I., Vieira M.L.C., Barroso P.A.V., Azevedo J.L. 2002. Genetic variability in the endophytic fungus Guignardia citricarpa isolated from citrus plants. Genetics and Molecular Biology 25: 251–255. https://doi.org/10.1590/S1415-47572002000200021 DOI: https://doi.org/10.1590/S1415-47572002000200021
  7. Glienke C., Pereira O., Stringari D., Fabris J., Kava-Cordeiro V., … Crous P.W. 2011. Endophytic and pathogenic Phyllosticta species, with reference to those associated with citrus black spot. Persoonia 26: 47–56. https://doi.org/10.3767/003158511X569169 DOI: https://doi.org/10.3767/003158511X569169
  8. Jiang A.M., Hou H.G., Jiang G.H., Fan C.L., Wei J.G., … Wei J.F., 2023. First report of leaf spot caused by Phyllosticta capitalensis on Illicium difengpi in China. Plant Disease 107: 937. https://doi.org/10.1094/PDIS-01-22-0164-PDN DOI: https://doi.org/10.1094/PDIS-01-22-0164-PDN
  9. Ji S.X., Zhou Y.Y., Zong X.Y., Li X.H., Zhang W., Yan J.Y., 2022. Toxicity test of thirteen fungicides on two main pathogens causing sweet cherry leaf spot disease. Northern Horticulture 18: 37–41. https://doi.org/10.11937/bfyy.20220934
  10. Jones D.T., Taylor W.R., Thormton J.M., 1992. The rapid generation of mutation data matrices from protein sequences. Computer Applications in the Biosciences 8(3): 275–282. https://doi.org/10.1093/bioinformatics/8.3.275 DOI: https://doi.org/10.1093/bioinformatics/8.3.275
  11. Landis M.J., Eaton D.A.R., Clement W.L., Park B., Spriggs E.L., … Donoghue M.J., 2021. Joint phylogenetic estimation of geographic movements and biome shifts during the global diversification of Viburnum. Systematic Biology 70: 67–85. https://doi.org/10.1093/sysbio/syaa027 DOI: https://doi.org/10.1093/sysbio/syaa027
  12. Li W., He Y.Q., Fu T., Lin L., Liu F., … Wang G.L., 2021a. First report of Colletotrichum siamense causing anthracnose on Zinnia elegans in China. Plant Disease 105: 1226. https://doi.org/10.1094/PDIS-04-20-0803-PDN DOI: https://doi.org/10.1094/PDIS-04-20-0803-PDN
  13. Li W., He Y.Q., Wang J.Y., Wang G.L., Wang Z.L., 2021b. Isolation and identification of pathogen causing anthracnose on Zinnia elegans Jacq. and screening of control fungicides. Chinese Journal of Pesticide Science 23(2): 341–347. https://doi.org/10.16801/j.issn.1008-7303.2021.0017
  14. Li H., Liao Y.C.Z., Wan Y., Li D.W., Zhu L.H., 2023. Colletotrichum siamense, a novel causal agent of Viburnum odoratissimum leaf blotch and its sensitivity to fungicides. Journal of Fungi 9: 882. https://doi.org/10.3390/jof9090882 DOI: https://doi.org/10.3390/jof9090882
  15. Ma Y.W., Ye L., Dong S.Y., Huai B.Y., Tan G.J., 2022. First report of Neofusicoccum parvum causing leaf spot on coral trees (Viburnum odoratissimum) in China. Plant Disease 106: 3000. https://doi.org/10.1094/PDIS-12-21-2799-PDN DOI: https://doi.org/10.1094/PDIS-12-21-2799-PDN
  16. Michael J.B., Uwe B., Donald H.P., 2022. Phylogeny and taxonomy of the genera of Erysiphaceae, part 1: Golovinomyces. Mycologia 114: 964–993. https://doi.org/10.1080/00275514.2022.2115419 DOI: https://doi.org/10.1080/00275514.2022.2115419
  17. Qiu C., Zhang Y., Liu Z., 2021. First Report of Alternaria spp. causing leaf spot on sweet viburnum in China. Plant Disease 105: 2253. https://doi.org/2253.10.1094/PDIS-06-20-1407-PDN DOI: https://doi.org/10.1094/PDIS-06-20-1407-PDN
  18. Shober A.L., Koeser A.K., Mclean D.C., Hasing G., Moore K.K., 2017. Nitrogen fertilizer rate, timing, and application method affects growth of sweet viburnum and nitrogen leaching from simulated planting beds. HortScience: A Publication of the American Society for Horticultural Science 52(1): 1–8. https://doi.org/10.21273/HORTSCI11114-16 DOI: https://doi.org/10.21273/HORTSCI11114-16
  19. Silva M., Pereira O.L., 2007. First report of Guignardia endophyllicola leaf blight on Cymbidium (Orchidaceae) in Brazil. Australasian Plant Disease Notes 2: 31–32. https://doi.org/10.1071/DN07015 DOI: https://doi.org/10.1071/DN07015
  20. Silva M., Pereira O.L., Braga I.F., Leli S.M., 2008. Leaf and pseudobulb diseases on Bifrenaria harrisoniae (Orchidaceae) caused by Phyllosticta capitalensis in Brazil. Australasian Plant Disease Notes 3: 53–56. https://doi.org/10.1071/DN08022 DOI: https://doi.org/10.1007/BF03211238
  21. Wan Y., Wu S., Si Y.Z., Li D.W., Zhu L.H., 2023. First report of Diaporthe eres causing leaf spot of Viburnum odoratissimum var. awabuki in China. Plant Disease 107: 954. https://doi.org/10.1094/PDIS-05-22-1187-PDN DOI: https://doi.org/10.1094/PDIS-05-22-1187-PDN
  22. Wang X., Chen G., Huang F., Zhang J., Hyde K.D., Li H., 2012. Phyllosticta species associated with citrus diseases in China. Fungal Diversity 52: 209–224. https://doi.org/10.1007/s13225-011-0140-y DOI: https://doi.org/10.1007/s13225-011-0140-y
  23. Wikee S., Lombard L., Crous P.W., Nakashima C., Motohashi K., … Hyde K.D., 2013. Phyllosticta capitalensis, a widespread endophyte of plants. Fungal Diversity 60: 91–105. https://doi.org/10.1007/s13225-013-0235-8 DOI: https://doi.org/10.1007/s13225-013-0235-8
  24. Wu S.P., Chen X.J., Liu Z.Y., 2015. Identification of Fatsia japonica grey spot pathogen and screening of fungicides. Southwest China Journal of Agricultural Sciences 28: 1130–1134.
  25. Yang X.X., Yue S.S., Gao L.X., Cheng J.S., Xie J.T., Fu Y.P., 2015. First report of anthracnose caused by Colletotrichum gloeosporioides on Viburnum odoratissimum in China. Plant Disease 99: 1647. https://doi.org/10.1094/PDIS-04-15-0417-PDN DOI: https://doi.org/10.1094/PDIS-04-15-0417-PDN
  26. Zhang T., Liu H., Song Q., Liu J., Yang Q., … Li D., 2022a. First report of leaf spot caused by Corynespora cassiicola on Viburnum odoratissimum var. awabuki (Sweet Viburnum) in China. Plant Disease 106: 1062. https://doi.org/10.1094/PDIS-04-21-0849-PDN DOI: https://doi.org/10.1094/PDIS-04-21-0849-PDN
  27. Zhang W.H., Su D., 2022b. First report of Phyllosticta capitalensis causing black freckle disease on Rubus chingii in China. Plant Disease 106: 1517. https://doi.org/10.1094/PDIS-05-21-1031-PDN DOI: https://doi.org/10.1094/PDIS-05-21-1031-PDN