Vol. 64 No. 1 (2025)
Articles

Isolation and identification of Fusarium spp. associated with Fusarium wilt of chickpea (Cicer arietinum L.) in Algeria

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  • Ibrahim SEKKAL,
  • Djamel MAHIOUT,
  • Boubekkeur Seddik BENDAHMANE,
  • Tahar FARAH,
  • Mohamed-Cherif BENTAHAR,
  • Martina RICKAUER
Ibrahim SEKKAL
Plant Protection Laboratory, Abdelhamid Ibn Badis University of Mostaganem, 27000 – Algeria
Djamel MAHIOUT
Plant Protection Laboratory, Abdelhamid Ibn Badis University of Mostaganem, 27000 – Algeria
Boubekkeur Seddik BENDAHMANE
Plant Protection Laboratory, Abdelhamid Ibn Badis University of Mostaganem, 27000 – Algeria
Tahar FARAH
Laboratory protection, Valorization of Coastal Marine Resources and Molecular Systematics, Abdelhamid Ibn Badis University of Mostaganem, 27000 – Algeria
Mohamed-Cherif BENTAHAR
Animal and Applied Physiology Laboratory, Abdelhamid Ibn Badis University of Mostaganem, 27000 – Algeria
Martina RICKAUER
Research Center of Biodiversity and Ecology, UMR5300, CNRS- Paul Sabatier University- National Polytechnic Institute INP-ENSAT, Avenue de l’Agrobiopole 31326 Castanet-Tolosan, France
DOI: https://doi.org/10.36253/phyto-15328
Categories

Published 2025-05-14

Keywords

  • Koch’s postulates,
  • transcription elongation factor 1-α gene

How to Cite

[1]
I. SEKKAL, D. MAHIOUT, B. S. BENDAHMANE, T. FARAH, M.-C. BENTAHAR, and M. RICKAUER, “Isolation and identification of Fusarium spp. associated with Fusarium wilt of chickpea (Cicer arietinum L.) in Algeria”, Phytopathol. Mediterr., vol. 64, no. 1, pp. 87–99, May 2025.

Copyright (c) 2025 Ibrahim SEKKAL, Djamel MAHIOUT, Boubekkeur Seddik BENDAHMANE, Tahar FARAH, Mohamed-Cherif BENTAHAR, Martina RICKAUER

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Abstract

Chickpea (Cicer arietinum L.) is an important vegetable crop in many Mediterranean countries, and Fusarium is known to cause wilt in these crops. Fusarium oxysporum f. sp. ciceris and Fusarium redolens are the only species which have been reported as the causes of Fusarium wilt in Algeria. Fusarium isolates (74) were obtained from roots of chickpea plants showing symptoms of wilting and necroses in xylem tissues. The plants were from 11 eleven principal provinces for chickpea crops in Algeria. Laboratory identifications for 31 isolates were achieved by sequencing their transcription elongation Factor 1- α (Ef-1 α) gene regions. Four principal species were identified including F. oxysporum (20 isolates), F. redolens (ten isolates), F. solani (one isolate) based on PCR using species-specific primers, and three formae speciales were identified within Fusarium oxysporum. Fusarium redolens and F. oxysporum f. sp. ciceris were dominant in the sampling sites.  Fusarium redolens was identified in the western region of Algeria, and F. oxysporum f. sp. ciceris was not isolated from this region. In contrast, from the eastern region, F. redolens was not isolated but F. oxysporum f. sp. ciceris was detected. Pathogenicity tests showed that chickpea was susceptible to various Fusarium species. Inoculated plants exhibited gradual wilting that eventually affected entire plants. This study provides new knowledge of the distribution of Fusarium species causing chickpea wilt in Algeria. Precise identification and the localization of pathogen species is important for developing Fusarium wilt management strategies, including development of wilt resistant chickpea cultivars.

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References

  1. Abdesselem S.M., Nisserine H.K., Mebrouk K., Jamal E.H., Jos Eacute S., Eduardo G., 2016. Characterization of Fusarium oxysporum isolates from tomato plants in Algeria. African Journal of Microbiology Research 10, 1156–1163. https://doi.org/10.5897/ajmr2016.8161 DOI: https://doi.org/10.5897/AJMR2016.8161
  2. Azil N., Stefanczyk E., Sobkowiak S., Chihat S., Boureghda H., Sliwka, J., 2021. Identification and pathogenicity of Fusarium spp. Associated with tuber dry rot and wilt of potato in Algeria. European Journal Plant Pathology 159, 495–509. https://doi.org/10.1007/s10658-020-02177-5 DOI: https://doi.org/10.1007/s10658-020-02177-5
  3. Baayen R.P., Gams W., 1988. The Elegans fusaria causing wilt disease of carnation. I. Taxonomy. Netherlands Journal of Plant Pathology 94: 273–288. DOI: https://doi.org/10.1007/BF01998052
  4. Benfreha F.Z., Henni D.E., Merzoug A., 2014. Fusarium wilt of Chickpea (Cicer arietinum L.) in Northwest Algeria. African Journal of Agricultural Research Vol. 9(1), 168-175, https://doi.org/10.5897/AJAR2013.6694 DOI: https://doi.org/10.5897/AJAR2013.6694
  5. Booth C., 1971. The Genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, UK. Reprinted 1977, 237 pp.
  6. Booth C., 1977. Fusarium Laboratory Guide to the Identification of the Major Species. Commonwealth Mycological Institute, Kew, Surrey, England, 58 pp.
  7. Bouhadida M., Jendoubi W., Gargouri S., Beji M., Kharrat M., Chen W., 2017. First report of Fusarium redolens causing Fusarium yellowing and wilt of chickpea in Tunisia. Plant Disease 101. https://doi.org/10.1094/PDIS-08-16-1114-PDN DOI: https://doi.org/10.1094/PDIS-08-16-1114-PDN
  8. Carbone I., Kohn L.M., 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553–556. https://doi.org/10.2307/3761358 DOI: https://doi.org/10.1080/00275514.1999.12061051
  9. Chehri K.h., Sattar H.A., 2018. Detection of fumonisin chemotype produced by Fusarium proliferatum isolated from nuts in Iraq using specific PCR assays. Biological Journal of Microorganism 24: 21–27.
  10. Chen L.H., Huang X.Q., Yang X.M., Shen Q.R., 2013. Modeling the effects of environmental factors on the population of Fusarium oxysporum in cucumber continuously cropped soil. Communications in Soil Science and Plant analysis 44: 2219–2232. DOI: https://doi.org/10.1080/00103624.2012.760577
  11. Couteaudier Y., Alabouvette C., 1990. Quantitative comparison of Fusarium oxysporum competitiveness in relation with carbon utilization. FEMS Microbiology Ecology 74: 261–268. DOI: https://doi.org/10.1111/j.1574-6968.1990.tb04072.x
  12. Doohan F.M., Brennan J., Cooke B.M., 2003. Influence of climatic factors on Fusarium species pathogenic to cereals. European Journal of Plant Pathology 109: 755–768. https://doi.org/10.1023/A:1026090626994 DOI: https://doi.org/10.1007/978-94-017-1452-5_10
  13. Edel-Hermann V., Gautheron N., Steinberg C., 2012. Genetic diversity of Fusarium oxysporum and related species pathogenic on tomato in Algeria and other Mediterranean countries. Plant Pathology 61: 787–800. https://doi.org/10.1111/j.1365-3059.2011.02551.x DOI: https://doi.org/10.1111/j.1365-3059.2011.02551.x
  14. Edel-Hermann V., Lecomte C., 2019. Current status of Fusarium oxysporum formae speciales and races. Phytopathology 109: 512–530. https://doi.org/10.1094/PHYTO-08-18-0320-RVW DOI: https://doi.org/10.1094/PHYTO-08-18-0320-RVW
  15. Elad Y., Baker R., 1985b. The role of competition for iron and carbon in suppression of chlamydospores germination of Fusarium spp. by Pseudomonas spp. Phytopathology 75: 1053–1059. DOI: https://doi.org/10.1094/Phyto-75-1053
  16. Felsenstein J., 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791. DOI: https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  17. Ficcadenti N., Sestili S., Annibali S., Campanelli G., Belisario A., … Corazza L., 2002. Resistance to Fusarium oxysporum f. sp. melonis Race 1, 2 in Muskmelon Lines. Plant Disease, 86: 897–900. DOI: https://doi.org/10.1094/PDIS.2002.86.8.897
  18. Gao H., Beckman C.H., Mueller W.C., 1995. The rate of vascular colonisation as a measure of the genotypic interaction between various cultivars of tomato various formae or races of Fusarium oxysporum. Physiological Molecular Plant Pathology 46: 29–43. DOI: https://doi.org/10.1006/pmpp.1995.1003
  19. Gatch E.W., du Toit L.J., 2017. Limestone-mediated suppression of Fusarium wilt in spinach seed crops. Plant Disease 101: 81–94. https://doi.org/10.1094/PDIS-04-16-0423-RE DOI: https://doi.org/10.1094/PDIS-04-16-0423-RE
  20. Gordon W.L., 1952. The occurrence of Fusarium species in Canada, II. Prevalence and taxonomy of Fusarium species in cereal seeds. Canadian Journal of Botany 30: 236–238. DOI: https://doi.org/10.1139/b52-018
  21. ISTA, 2015. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.
  22. Jalali B.L., Chand H., 1992. Chickpea wilt. In: Plant Diseases of International Importance.. Diseases of Cereals and Pulses. Vol. I (Singh U.S., Mukhopadhayay A.N., Kumar J., Chaube H.S., ed). Prentice Hall, Englewood Cliffs, NJ, USA. 429–444.
  23. Jimenez-Diaz R.M., Singh K.B., Trapero-Casas A., 1989. Races of Fusarium oxysporum f. sp. ciceri infecting chickpeas in southern Spain. In: Vascular Wilt Diseases of Plants (Tjamos E.C., Beckman C.H. ed.), NATO ASI Ser., Springer-Berlin, 1989, vol. H28, 515–520. DOI: https://doi.org/10.1007/978-3-642-73166-2_39
  24. Jiménez-Díaz, R.M., Porta-Puglia A., Tivoli B., 1998. New approaches in the integrated management of legume diseases: Toward sustainable crop health. Pages 89–93. In: European Conference Grain Legumes, 3rd. Opportunities for High Quality, Healthy and Added-value Crops to Meet European Demands. European Association for Grain Legumes, ed. Valladolid, Spain.
  25. Jiménez-Díaz R.M., Singh K.B., Trapero-Casas A., Trapero-Casas, JL, 1991. Resistance in kabuli chickpeas to Fusarium wilt. Plant Disease 75: 914–918. DOI: https://doi.org/10.1094/PD-75-0914
  26. Jimenez-Fernandez D., Navas-Cortes J.A., Montes-Borrego M., Jiménez-Díaz RM, Landa B.B., 2011. Molecular and pathogenic characterization of Fusarium redolens, a new causal agent of Fusarium yellows in chickpea. Plant Disease 95: 860–870. https://doi.org/10.1094/PDIS-12-10-0946 DOI: https://doi.org/10.1094/PDIS-12-10-0946
  27. Jiménez-Gasco M.M., Jiménez-Díaz R.M., 2003. Development of a specific polymerase chain reaction based-assay for the identification of Fusarium oxysporum f. sp. ciceris and its pathogenic races 0, 1A, 5 and 6. Phytopathology 93: 200–209. https://doi.org/10.1094/PHYTO.2003.93.2.200 DOI: https://doi.org/10.1094/PHYTO.2003.93.2.200
  28. Korter G.O., Sunday A.A., Ehiagwina F.O., Lawal P.O., Akinola T.E., Kolawole S.K., 2023. Comparative studies of rockwool and cotton wool for tomatoes production: Nutrient balance, plant growth and fruit quality. International Research Journal of Modernization in Engineering Technology and Science 5: 19–25.
  29. Landa B.B., Navas-Cortés J.A., Jiménez-Díaz R.M., 2004. Integrated management of Fusarium wilt of chickpea with sowing date, host resistance, and biological control. Phytopathology 94: 946–960. https://doi.org/10.1094/PHYTO.2004.94.9.946 DOI: https://doi.org/10.1094/PHYTO.2004.94.9.946
  30. Landa B.B., Navas-Cortés J.A., Jiménez-Gasco M.M., Katan J., Retig B., Jiménez-Díaz R.M., 2006. Temperature response of chickpea cultivars to races of Fusarium oxysporum f. sp. ciceris, causal agent of Fusarium wilt. Plant Disease 90: 365–374. https://doi.org/10.1094/PD-90-0365 DOI: https://doi.org/10.1094/PD-90-0365
  31. Lazreg F., Belabid L., Sanchez J., Gallego E., Bayaa B., 2014. Pathogenicity of Fusarium spp. associated with diseases of Aleppo-pine seedlings in Algerian forest nurseries. Journal of Forest Science 60:1 15–120. DOI: https://doi.org/10.17221/65/2013-JFS
  32. Lazreg F., Belabid L., Sanchez J., Gallego E., Garrido-Cardenas J.A., Elhaitoum A., 2013. First Report of Fusarium redolens as a Causal Agent of Aleppo Pine Damping-Off in Algeria. Plant Disease 97: 997. https://doi.org/10.1094/PDIS-12-12-1169-PDN DOI: https://doi.org/10.1094/PDIS-12-12-1169-PDN
  33. Leslie J.F., Zeller K.A., Summerell B.A., 2001. Icebergs and species in populations of Fusarium. Physiological and Molecular Plant Pathology. 59: 107–117. https://doi.org/10.1006/pmpp.2001.0351 DOI: https://doi.org/10.1006/pmpp.2001.0351
  34. Merga B., Haji J., 2019. Economic importance of chickpea: production, value, and world trade. Cogent Food & Agriculture, vol. 5 (1). https://doi.org/10.1080/23311932.2019.1615718 DOI: https://doi.org/10.1080/23311932.2019.1615718
  35. Navas-Cortés J.A., Hau B., Jiménez-Díaz, R.M., 1998. Effect of sowing date, host cultivar, and race of Fusarium oxysporum f. sp. ciceris on development of Fusarium wilt of chickpea. Phytopathology 88: 1338–1346. https://doi.org/10.1094/PHYTO.1998.88.12.1338 DOI: https://doi.org/10.1094/PHYTO.1998.88.12.1338
  36. Navas-Cortés J.A., Hau B., Jiménez-Díaz, R.M., 2000. Yield loss in chickpeas in relation to development of Fusarium wilt epidemics. Phytopathology 90: 1269–1278. https://doi.org/10.1094/PHYTO.2000.90.11.1269 DOI: https://doi.org/10.1094/PHYTO.2000.90.11.1269
  37. Nelson P.E., Toussoun T.A., Marasas W.F.O., 1983. Fusarium species: An illustrated Manual for Identification. Pennsylvania State University Press, University Park, USA.
  38. Nene Y.L., Reddy M.V., 1987. Chickpea diseases and their control. In: The Chickpea. (Saxena M.C., Singh K.B., ed.). Oxon, UK. CAB-International, 233–270.
  39. Nene Y.L., Reddy M.V., Haware M.P., Ghanekar A.M., Amin K.S., … Sharma M., 2012. Field Diagnosis of Chickpea Diseases and their Control. Information Bulletin No. 28 (revised). International Crops Research Institute for the Semi-Arid Tropics. 60 pp.
  40. Parry D.W., Jenkinson P., McLeod L., 1995. Fusarium ear blight (scab) in small grain cereals – a review. Plant Pathology. https://doi.org/10.1111/j.1365-3059.1995.tb02773.x DOI: https://doi.org/10.1111/j.1365-3059.1995.tb02773.x
  41. Ramirez-Suero M., Khanshour A., Martinez Y., Rickauer M., 2010. A study on the susceptibility of the model legume plant Medicago truncatula to the soil-borne pathogen Fusarium oxysporum. European Journal Plant Pathology 126: 517–530. https://doi.org/10.1007/s10658-009-9560-x DOI: https://doi.org/10.1007/s10658-009-9560-x
  42. Rossi V., Ravanetti A., Pattori E., Giosuè S., 2001. Influence of temperature and humidity on the infection of wheat spikes by some fungi causing Fusarium head blight. Journal of Plant Pathology 83: 189–198. https://doi.org/10.4454/JPP.V83I3.1128
  43. Sanger F., Nicklen S., Coulson AR, 1977. DNA sequencing with chain-terminating inhibitors. Proceedings National Academy Sciences USA 74 (12): 5463–7. Bib code: 1977PNAS...74.5463S. https://doi.org/10.1073/pnas.74.12.5463. PMC 431765. PMID 271968 DOI: https://doi.org/10.1073/pnas.74.12.5463
  44. Siddique K.H.M., Brinsmead R.B., Knight R., Knights E.J., Paull J.G., Rose I.A., 2000. Adaptation of chickpea (Cicer arietinum L.) and faba bean (Vicia faba L.) to Australia. In R. Knight (Ed.), Linking Research and Marketing Opportunities for Pulses in the 21st Century (289–303). Dordrecht: Kluwe Academic Publishers. https://doi.org/10.1007/978-94-011-4385-1_26 DOI: https://doi.org/10.1007/978-94-011-4385-1_26
  45. Sneath P.H.A. and Sokal R.R. 1973. Numerical Taxonomy. Freeman, San Francisco. 600 pp.
  46. Taheri A.E., 2011. First report of Fusarium redolens from Saskatchewan and its comparative pathogenicity. Canadian Journal Plant Pathology 33: 559. https://doi.org/10.1080/07060661.2011.620631 DOI: https://doi.org/10.1080/07060661.2011.620631
  47. Tamura K., Nei M., Kumar S., 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101: 11030–11035. DOI: https://doi.org/10.1073/pnas.0404206101
  48. Tamura K., Stecher G., Kumar S. 2021. MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution. https://doi.org/10.1093/molbev/msab120. DOI: https://doi.org/10.1093/molbev/msab120
  49. Tekeoğlu M., Özkılınç H., Tunalı B., Kusmenoğlu İ., Chen W., 2017. Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey Mediterranean Agricultural Sciences 30 (1): 27–33. https://doi.org/10.22268/AJPP-39.4.231240 DOI: https://doi.org/10.22268/AJPP-39.4.231240
  50. Tlemsani M., Bellahcene M., Fortas Z., 2015. Behavior study of chickpeas varieties (Cicer arietinum L.) against Fusarium wilt. Asian Journal of Biochemical and Pharmaceutical Research.
  51. Zaim S., 2016. Essai de lutte biologique contre le Fusarium oxysporum f. sp. ciceris à l’aide des microorganismes de la rhizosphère de la culture du pois chiche. PhD Thesis, University of Mascara, Mascara, Algeria, 220 pp.
  52. Zaim S., Bekkar A.A., 2022. First report of Fusarium redolens causing Fusarium yellows on chickpea in Algeria. Journal of Plant Pathology 104(2). https://doi.org/10.1007/s42161-022-01044-y DOI: https://doi.org/10.1007/s42161-022-01044-y
  53. Zhuang W.Y., Guo L., Guo S.Y., Guo Y.L., Mao X.L., … Zhang X.Q., 2005. Fungi of Northwestern China. Ithaca (NY): Mycotaxon, 430 pp.

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