Vol. 63 No. 2 (2024)
Articles

Genetic variability of grapevine Pinot gris virus (GPGV) in an organically cultivated vineyard in Hungary

PDF Suppl.
  • Réka SÁRAY,
  • Erzsébet SZATHMÁRY,
  • Dóra PINCZÉS,
  • Asztéria ALMÁSI,
  • Tamás DEÁK,
  • László PALKOVICS,
  • Katalin SALÁNKI
Réka SÁRAY
Department of Plant Pathology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Budapest
Erzsébet SZATHMÁRY
Department of Plant Pathology, Institute of Plant Protection, Hungarian University of Agriculture and Life Sciences (MATE), Budapest
Dóra PINCZÉS
Department of Plant Pathology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Budapest
Asztéria ALMÁSI
Department of Plant Pathology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Budapest
Tamás DEÁK
Department of Viticulture, Institute of Viticulture and Enology, Hungarian University of Agriculture and Life Sciences (MATE), Budapest
László PALKOVICS
ELKH-SZE PhatoPlant-Lab, Széchenyi Isván University, Mosonmagyaróvár
Katalin SALÁNKI
Department of Plant Pathology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Budapest
DOI: https://doi.org/10.36253/phyto-14492

Published 2024-07-17

Keywords

  • RT-PCR,
  • phylogenetic analysis,
  • sequence analysis

How to Cite

[1]
SÁRAY R., “Genetic variability of grapevine Pinot gris virus (GPGV) in an organically cultivated vineyard in Hungary”, Phytopathol. Mediterr., vol. 63, no. 2, pp. 179–190, Jul. 2024.

Copyright (c) 2024 Réka SÁRAY, Erzsébet SZATHMÁRY, Dóra PINCZÉS, Asztéria ALMÁSI, Tamás DEÁK, László PALKOVICS, Katalin SALÁNKI

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Grapevine Pinot gris virus (GPGV) is a recently identified trichovirus infecting grapevines. Despite wide distribution, there is limited available information on epidemiology, transmission, and associated symptoms of grapevine leaf mottling and deformation. Occurrence and genetic diversity of GPGV variants were surveyed in an organically cultivated Hungarian vineyard that was planted between 1996 and 2014. Sequence analysis demonstrated the widespread presence and high variability of GPGV, and according to phylogenetic analyses, the Hungarian virus isolates were classified into three groups. Most of the identified variants clustered with the representative asymptomatic isolates, but all isolates from one grapevine cultivar grouped with representative isolates of clade B. Furthermore, one isolate clustered with representative isolates of clade C, and the identified clade C variant had previously undescribed polymorphisms.

PDF Suppl.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

  1. Abe J., Nabeshima T., 2021. First report of grapevine Pinot gris virus in wild grapevines (Vitis coignetiae) in Japan. Journal of Plant Pathology 103: 725–725. https://doi.org/10.1007/s42161-021-00800-w DOI: https://doi.org/10.1007/s42161-021-00800-w
  2. Abou Kubaa R., Lanotte P., Saldarelli P., 2019. First report of grapevine Pinot gris virus in grapevine in Moldavia. Journal of Plant Pathology 101: 441–441. https://doi.org/10.1007/s42161-018-00209-y DOI: https://doi.org/10.1007/s42161-018-00209-y
  3. Abou Kubaa R., Choueiri E., Jreijiri F., El Khoury Y., Saldarelli P., 2020. First report of grapevine Pinot gris virus in Lebanon and the Middle East. Journal of Plant Pathology 102: 565–565. https://doi.org/10.1007/s42161-019-00453-w DOI: https://doi.org/10.1007/s42161-019-00453-w
  4. Al Rwahnih M., Golino D., Rowhani A., 2016. First Report of Grapevine Pinot gris virus Infecting Grapevine in the United States. Plant Disease 100: 1030–1030. https://doi.org/10.1094/PDIS-10-15-1235-PDN DOI: https://doi.org/10.1094/PDIS-10-15-1235-PDN
  5. Bertazzon N., Filippin L., Forte V., Angelini E., 2016. Grapevine Pinot gris virus seems to have recently been introduced to vineyards in Veneto, Italy. Archives of Virology 161: 711–714. https://doi.org/10.1007/s00705-015-2718-2 DOI: https://doi.org/10.1007/s00705-015-2718-2
  6. Bertazzon N., Forte V., Filippin L., Causin R., Maixner M., Angelini E., 2017. Association between genetic variability and titre of Grapevine Pinot gris virus with disease symptoms. Plant Pathology 66: 949–959. https://doi.org/10.1111/ppa.12639 DOI: https://doi.org/10.1111/ppa.12639
  7. Bertazzon N., Forte V., Angelini E., 2020. Fast transmission of grapevine “Pinot gris” virus (GPGV) in vineyard. VITIS - Journal of Grapevine Research 29-34 Pages. https://doi.org/10.5073/VITIS.2020.59.29-34.
  8. Bertazzon N., Angelini E., Signorotto M., Genov N., 2021a. First report of grapevine Pinot gris virus and grapevine leafroll-associated virus 2 in Bulgarian vineyards. Journal of Plant Diseases and Protection 128: 597–599. https://doi.org/10.1007/s41348-020-00415-w DOI: https://doi.org/10.1007/s41348-020-00415-w
  9. Bertazzon N., Rahali M., Angelini E., Crespan M., Migliaro D., 2021b. First Report of Grapevine Pinot gris virus Infecting Grapevine in Algeria. Plant Disease 105: 234. https://doi.org/10.1094/PDIS-04-20-0723-PDN DOI: https://doi.org/10.1094/PDIS-04-20-0723-PDN
  10. Beuve M., Candresse T., Tannières M., Lemaire O., 2015. First Report of Grapevine Pinot gris virus (GPGV) in grapevine in France. Plant Disease 99: 293–293. https://doi.org/10.1094/PDIS-10-14-1008-PDN DOI: https://doi.org/10.1094/PDIS-10-14-1008-PDN
  11. Bianchi G.L., De Amicis F., De Sabbata L., Di Bernardo N., Governatori G., … Frausin C., 2015. Occurrence of Grapevine Pinot gris virus in Friuli Venezia Giulia (Italy): field monitoring and virus quantification by real-time RT-PCR. EPPO Bulletin 45: 22–32. https://doi.org/10.1111/epp.12196 DOI: https://doi.org/10.1111/epp.12196
  12. Casati P., Maghradze D., Quaglino F., Ravasio A., Failla O., Bianco P.A., 2015. First report of grapevine pinot gris virus in Georgia. Journal of Plant Pathology 97, S67.
  13. Czotter N., Molnar J., Szabó E., Demian E., Kontra L., … Varallyay E., 2018. NGS of Virus-Derived Small RNAs as a Diagnostic Method Used to Determine Viromes of Hungarian Vineyards. Frontiers in Microbiology 9. https://doi.org/10.3389/fmicb.2018.00122 DOI: https://doi.org/10.3389/fmicb.2018.00122
  14. Debat H., Luna F., Moyano S., Zavallo D., Asurmendi S., Gomez-Talquenca S., 2020. First report of grapevine Pinot gris virus infecting grapevine in Argentina. Journal of Plant Pathology 102: 1321–1321. https://doi.org/10.1007/s42161-020-00608-0 DOI: https://doi.org/10.1007/s42161-020-00608-0
  15. Demian E., Jaksa-Czotter N., Varallyay E., 2022. Grapevine Pinot Gris Virus Is Present in Different Non-Vitis Hosts. Plants 11(14): 1830. https://doi.org/10.3390/plants11141830 DOI: https://doi.org/10.3390/plants11141830
  16. Eichmeier A., Peňázová E., Pavelková R., Mynarzová Z., Saldarelli P., 2016. Detection of Grapevine Pinot Gris Virus in Certified Grapevine Stocks in Moravia, Czech Republic. Journal of Plant Pathology 98: 155–157.
  17. Eichmeier A., Pieczonka K., Peňázová E., Pečenka J., Gajewski Z., 2017. Occurrence of Grapevine Pinot gris virus in Poland and description of asymptomatic exhibitions in grapevines. Journal of Plant Diseases and Protection 124: 407–411. https://doi.org/10.1007/s41348-017-0076-x DOI: https://doi.org/10.1007/s41348-017-0076-x
  18. Eichmeier A., Peňázová E., Muljukina N., 2018. Survey of Grapevine Pinot gris virus in certified grapevine stocks in Ukraine. European Journal of Plant Pathology 152: 555–560. https://doi.org/10.1007/s10658-018-1497-5 DOI: https://doi.org/10.1007/s10658-018-1497-5
  19. Eichmeier A., Peňázová E., Čechová J., Berraf-Tebbal A., 2020. Survey and Diversity of Grapevine Pinot gris virus in Algeria and Comprehensive High-Throughput Small RNA Sequencing Analysis of Two Isolates from Vitis vinifera cv. Sabel Revealing High Viral Diversity. Genes 11: 1110. https://doi.org/10.3390/genes11091110 DOI: https://doi.org/10.3390/genes11091110
  20. Fajardo T.V.M., Eiras M., Nickel O., 2017. First report of Grapevine Pinot gris virus infecting grapevine in Brazil. Australasian Plant Disease Notes 12: 45. https://doi.org/10.1007/s13314-017-0270-5 DOI: https://doi.org/10.1007/s13314-017-0270-5
  21. Fan X.D., Dong Y.F., Zhang Z.P., Ren F., Hu G.J., … Zhou J., 2016. First Report of Grapevine Pinot gris virus in Grapevines in China. Plant Disease 100: 540–540. https://doi.org/10.1094/PDIS-08-15-0913-PDN DOI: https://doi.org/10.1094/PDIS-08-15-0913-PDN
  22. Gambino G., Gribaudo I., 2006. Simultaneous Detection of Nine Grapevine Viruses by Multiplex Reverse Transcription-Polymerase Chain Reaction with Coamplification of a Plant RNA as Internal Control. Phytopathology 96: 1223–1229. https://doi.org/10.1094/PHYTO-96-1223 DOI: https://doi.org/10.1094/PHYTO-96-1223
  23. Gazel M., Caglayan K., Elçi E., Öztürk L., 2016. First Report of Grapevine Pinot gris virus in Grapevine in Turkey. Plant Disease 100: 657–657. https://doi.org/10.1094/PDIS-05-15-0596-PDN DOI: https://doi.org/10.1094/PDIS-05-15-0596-PDN
  24. Gentili A., Prota V., Moro G., Schianchi N., Di Lucca E., … Faggioli F., 2017. Identification of Grapevine Pinot Gris Virus in Sardinia and Lazio (south and Central Italy). Journal of Plant Pathology 99: 527–530.
  25. Giampetruzzi A., Roumi V., Roberto R., Malossini U., Yoshikawa N., … Saldarelli P., 2012. A new grapevine virus discovered by deep sequencing of virus- and viroid-derived small RNAs in Cv Pinot gris. Virus Research 163: 262–268. https://doi.org/10.1016/j.virusres.2011.10.010 DOI: https://doi.org/10.1016/j.virusres.2011.10.010
  26. Glasa M., Predajňa L., Komínek P., Nagyová A., Candresse T., Olmos A., 2014. Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines. Archives of Virology 159: 2103–2107. https://doi.org/10.1007/s00705-014-2031-5 DOI: https://doi.org/10.1007/s00705-014-2031-5
  27. Gualandri V., Asquini E., Bianchedi P., Covelli L., Brilli M., … Si-Ammour A., 2017. Identification of herbaceous hosts of the Grapevine Pinot gris virus (GPGV). European Journal of Plant Pathology 147: 21–25. https://doi.org/10.1007/s10658-016-0989-4 DOI: https://doi.org/10.1007/s10658-016-0989-4
  28. Hily J.-M., Poulicard N., Candresse T., Vigne E., Beuve M., … Lemaire O., 2020. Datamining, Genetic Diversity Analyses, and Phylogeographic Reconstructions Redefine the Worldwide Evolutionary History of Grapevine Pinot gris virus and Grapevine berry inner necrosis virus. Phytobiomes Journal 4: 165–177. https://doi.org/10.1094/PBIOMES-10-19-0061-R DOI: https://doi.org/10.1094/PBIOMES-10-19-0061-R
  29. Hily J.-M., Komar V., Poulicard N., Velt A., Renault L., … Lemaire O., 2021a. Evidence of differential spreading events of grapevine pinot Gris virus in Italy using datamining as a tool. European Journal of Plant Pathology 161: 735–742. https://doi.org/10.1007/s10658-021-02343-3 DOI: https://doi.org/10.1007/s10658-021-02343-3
  30. Hily J.-M., Komar V., Poulicard N., Vigne E., Jacquet O., … Lemaire O., 2021b. Biological Evidence and Molecular Modeling of a Grapevine Pinot gris Virus Outbreak in a Vineyard. Phytobiomes Journal 5: 464–472. https://doi.org/10.1094/PBIOMES-11-20-0079-R. DOI: https://doi.org/10.1094/PBIOMES-11-20-0079-R
  31. Jo Y., Choi H., Kyong Cho J., Yoon J.-Y., Choi S.-K., Kyong Cho W., 2015. In silico approach to reveal viral populations in grapevine cultivar Tannat using transcriptome data. Scientific Reports 5: 15841. https://doi.org/10.1038/srep15841 DOI: https://doi.org/10.1038/srep15841
  32. Kiss T., Kocanová M., Vavřiník A., Tekielska D., Pečenka J., … Eichmeier A., 2021. Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes. Agronomy 11: 1020. https://doi.org/10.3390/agronomy11061020 DOI: https://doi.org/10.3390/agronomy11061020
  33. Martelli G.P., 2014. Directory of virus and virus-like diseases of the grapevine and their agents. Journal of Plant Pathology 96: 1–136. DOI: https://doi.org/10.1002/9780470015902.a0000766.pub3
  34. Massart S., Vankerkoven L., Blouin A.G., Nourinejhad Zarghani S., Wetzel T., 2020. First Report of Grapevine Pinot Gris Virus and Grapevine Rupestris Stem Pitting-Associated Virus in Grapevine in Belgium. Plant Disease 104: 1879. https://doi.org/10.1094/PDIS-10-19-2071-PDN DOI: https://doi.org/10.1094/PDIS-10-19-2071-PDN
  35. Morán F., Olmos A., Lotos L., Predajňa L., Katis N., … Ruiz-García A.B., 2018. A novel specific duplex real-time RT-PCR method for absolute quantitation of Grapevine Pinot gris virus in plant material and single mites. PLOS ONE 13: e0197237. https://doi.org/10.1371/journal.pone.0197237 DOI: https://doi.org/10.1371/journal.pone.0197237
  36. Morelli M., Catarino A. de M., Susca L., Saldarelli P., Gualandri V., Martelli G.P., 2014. First report of Grapevine pinot gris virus from table grapes in southern Italy. Journal of Plant Pathology 96.
  37. Oñate-Sánchez L., Vicente-Carbajosa J., 2008. DNA-free RNA isolation protocols for Arabidopsis thaliana, including seeds and siliques. BMC Research Notes 1: 93. https://doi.org/10.1186/1756-0500-1-93 DOI: https://doi.org/10.1186/1756-0500-1-93
  38. Pleško I.M., Marn M.V., Seljak G., Žežlina I., 2014. First Report of Grapevine Pinot gris virus Infecting Grapevine in Slovenia. Plant Disease 98: 1014–1014. https://doi.org/10.1094/PDIS-11-13-1137-PDN DOI: https://doi.org/10.1094/PDIS-11-13-1137-PDN
  39. Poojari S., Lowery T., Rott M., Schmidt A.-M., Úrbez-Torres J.R., 2016. First Report of Grapevine Pinot gris virus in British Columbia, Canada. Plant Disease 100: 1513–1513. https://doi.org/10.1094/PDIS-02-16-0178-PDN DOI: https://doi.org/10.1094/PDIS-02-16-0178-PDN
  40. Rasool S., Naz S., Rowhani A., Golino D.A., Westrick N.M., … Al Rwahnih M., 2017. First Report of Grapevine Pinot gris virus Infecting Grapevine in Pakistan. Plant Disease 101: 1958–1958. https://doi.org/10.1094/PDIS-04-17-0476-PDN DOI: https://doi.org/10.1094/PDIS-04-17-0476-PDN
  41. Reynard J.-S., Schumacher S., Menzel W., Fuchs J., Bohnert P., … Fuchs R., 2016. First Report of Grapevine Pinot gris virus in German Vineyards. Plant Disease 100: 2545–2545. https://doi.org/10.1094/PDIS-07-16-0966-PDN DOI: https://doi.org/10.1094/PDIS-07-16-0966-PDN
  42. Ruiz-García A.B., Olmos A., 2017. First Report of Grapevine Pinot gris virus in Grapevine in Spain. Plant Disease 101: 1070. https://doi.org/10.1094/PDIS-12-16-1858-PDN DOI: https://doi.org/10.1094/PDIS-12-16-1858-PDN
  43. Saldarelli P., Giampetruzzi A., Morelli M., Malossini U., Pirolo C., … Gualandri V., 2015. Genetic Variability of Grapevine Pinot gris virus and Its Association with Grapevine Leaf Mottling and Deformation. Phytopathology 105: 555–563. https://doi.org/10.1094/PHYTO-09-14-0241-R DOI: https://doi.org/10.1094/PHYTO-09-14-0241-R
  44. Shvets D., Vinogradova S., 2022. Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia. Plants 11: 1061. https://doi.org/10.3390/plants11081061. DOI: https://doi.org/10.3390/plants11081061
  45. Silva G., Lecourt J., Clover G.R.G., Seal S.E., 2018. First record of Grapevine Pinot gris virus infecting Vitis vinifera in the United Kingdom. New Disease Reports 38: 7. https://doi.org/10.5197/j.2044-0588.2018.038.007 DOI: https://doi.org/10.5197/j.2044-0588.2018.038.007
  46. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30: 2725–2729. https://doi.org/10.1093/molbev/mst197 DOI: https://doi.org/10.1093/molbev/mst197
  47. Tarquini G., Ermacora P., Bianchi G.L., De Amicis F., Pagliari L., … Musetti R., 2018. Localization and subcellular association of Grapevine Pinot Gris Virus in grapevine leaf tissues. Protoplasma 255: 923–935. https://doi.org/10.1007/s00709-017-1198-5 DOI: https://doi.org/10.1007/s00709-017-1198-5
  48. Tarquini G., De Amicis F., Martini M., Ermacora P., Loi N., … Firrao G., 2019a. Analysis of new grapevine Pinot gris virus (GPGV) isolates from Northeast Italy provides clues to track the evolution of a newly emerging clade. Archives of Virology 164: 1655–1660. https://doi.org/10.1007/s00705-019-04241-w DOI: https://doi.org/10.1007/s00705-019-04241-w
  49. Tarquini G., Zaina G., Ermacora P., Amicis F.D., Franco-Orozco B., … Musetti R., 2019b. Agroinoculation of Grapevine Pinot Gris Virus in tobacco and grapevine provides insights on viral pathogenesis. PLOS ONE 14: e0214010. https://doi.org/10.1371/journal.pone.0214010 DOI: https://doi.org/10.1371/journal.pone.0214010
  50. Tarquini G., Ermacora P., Firrao G., 2021a. Polymorphisms at the 3’end of the movement protein (MP) gene of grapevine Pinot gris virus (GPGV) affect virus titre and small interfering RNA accumulation in GLMD disease. Virus Research 302: 198482. https://doi.org/10.1016/j.virusres.2021.198482 DOI: https://doi.org/10.1016/j.virusres.2021.198482
  51. Tarquini G., Pagliari L., Ermacora P., Musetti R., Firrao G., 2021b. Trigger and Suppression of Antiviral Defenses by Grapevine Pinot Gris Virus (GPGV): Novel Insights into Virus-Host Interaction. Molecular Plant-Microbe Interactions 34: 1010–1023. https://doi.org/10.1094/MPMI-04-21-0078-R DOI: https://doi.org/10.1094/MPMI-04-21-0078-R
  52. Tarquini G., Ermacora P., Martini M., Firrao G., 2023. The Conundrum of the Connection of Grapevine Pinot Gris Virus with the Grapevine Leaf Mottling and Deformation Syndrome. Plant Pathology 72: 209–217. https://doi.org/10.1111/ppa.13667 DOI: https://doi.org/10.1111/ppa.13667
  53. Tokhmechi K., Koolivand D., 2020. First report of grapevine Pinot gris virus infecting grapevine in Iran. Journal of Plant Pathology 102: 549–549. https://doi.org/10.1007/s42161-019-00437-w DOI: https://doi.org/10.1007/s42161-019-00437-w
  54. Waterhouse A.M., Procter J.B., Martin D.M.A., Clamp M., Barton G.J., 2009. Jalview Version 2—a multiple sequence alignment editor and analysis workbench. Bioinformatics 25: 1189–1191. https://doi.org/10.1093/bioinformatics/btp033 DOI: https://doi.org/10.1093/bioinformatics/btp033
  55. Wu Q., Habili N., 2017. The recent importation of Grapevine Pinot gris virus into Australia. Virus Genes 53: 935–938. https://doi.org/10.1007/s11262-017-1475-6. DOI: https://doi.org/10.1007/s11262-017-1475-6
  56. Xiao H., Shabanian M., McFadden-Smith W., Meng B., 2016. First Report of Grapevine Pinot gris virus in Commercial Grapes in Canada. Plant Disease 100: 1030–1030. https://doi.org/10.1094/PDIS-12-15-1405-PDN DOI: https://doi.org/10.1094/PDIS-12-15-1405-PDN
  57. Xu Q., Wen X., Deng X., 2004. A simple protocol for isolating genomic DNA from chestnut rose (Rosa roxburghii tratt) for RFLP and PCR analyses. Plant Molecular Biology Reporter 22: 301–302. https://doi.org/10.1007/BF02773140 DOI: https://doi.org/10.1007/BF02773140
  58. Zamorano A., Medina G., Fernández C., Cui W., Quiroga N., Fiore N., 2019. First Report of Grapevine Pinot gris virus in Grapevine in Chile. Plant Disease 103: 1438. https://doi.org/10.1094/PDIS-10-18-1855-PDN DOI: https://doi.org/10.1094/PDIS-10-18-1855-PDN