Articles
Virulence, genetic diversity, and putative geographical origin of sunflower broomrape populations in Morocco
Abdelghani NABLOUSSI- Begoña PÉREZ-VICH
- Leonardo VELASCO
Published 2023-05-08
Keywords
- Genetic diversity,
- parasitic weeds,
- SNP markers,
- virulence,
- plant introductions
How to Cite
[1]
A. NABLOUSSI, PÉREZ-VICH B., and L. VELASCO, “Virulence, genetic diversity, and putative geographical origin of sunflower broomrape populations in Morocco”, Phytopathol. Mediterr., vol. 62, no. 1, pp. 65–72, May 2023.
Copyright (c) 2023 Abdelghani NABLOUSSI, Begoña PÉREZ-VICH, Leonardo VELASCO
This work is licensed under a Creative Commons Attribution 4.0 International License.
Funding data
-
Ministerio de Ciencia e Innovación
Grant numbers PID2020-117286RB-I00
Abstract
Sunflower broomrape (Orobanche cumana Wallr.) was detected for the first time parasitizing sunflower in Morocco in 2016. Seeds of three broomrape populations from two separate areas of Morocco, Souk Al Arbaa (populations SA1 and SA2) and Meknès (Population MK1) were collected. The populations’ virulence, genetic diversity, and putative area of origin were examined. Race classification using a set of sunflower differential lines showed that MK1 was a race-E population, while SA1 and SA2 were race-G populations. The analysis with 192 SNP markers showed that SA1 and SA2 populations are genetically similar and very distant from the MK1 population. The three populations exhibited low intrapopulation diversity. Comparisons with populations from other areas showed that MK1 was introduced from a race-E population from the Guadalquivir Valley gene pool in Southern Spain, probably before 1988. Populations SA1 and SA2 showed close relationships with a population from Russia, although more exact knowledge of the origin of these populations requires further investigation. Since the SA and MK populations were collected from areas located approx. 100 km apart, the risks of mixing and recombining both gene pools to produce more virulent variants must be considered.
Downloads
Download data is not yet available.
Metrics
Metrics Loading ...
References
Amri M., Abbes Z., Youssef S.B., Bouhadida M., Salah H.B., Kharrat M., 2012. Detection of the parasitic plant, Orobanche cumana on sunflower (Helianthus annuus L.) in Tunisia. African Journal of Biotechnology. 11: 4163–4167. https://doi.org/10.5897/AJB11.3031
Antonova T., 2014. The history of interconnected evolution of Orobanche cumana Wallr. and sunflower in the Russian Federation and Kazakhstan. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 57−64.
Antonova T.S., Araslanova N.M., Strelnikov E.A., Ramazanova S.A., Guchetl S.Z., Chelyustnikova T.A., 2013. Distribution of highly virulent races of sunflower broomrape (Orobanche cumana Wallr.) in the southern regions of the Russian Federation. Russian Agricultural Sciences 39: 46–50. https://doi.org/10.3103/S1068367413010023
Batchvarova R., 2014. Current situation of sunflower broomrape in Bulgaria. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 51−54.
Beck-Mannagetta G., 1930. Orobanchaceae. In: Das Pflanzenreich Vol. IV (A. Engler, ed.), Verlag von Wilhelm Engelmann, Leipzig, 1−348.
Bilgen B.B., Barut A.K., Demirbaş S., 2019. Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers. Turkish Journal of Botany 43: 3. https://doi.org/10.3906/bot-1807-71
Calderón-González, A., Pouilly N., Muños S., Grand X., Coque M., Velasco L., Pérez-Vich B., 2019. A SSR-SNP linkage map of the parasitic weed Orobanche cumana Wallr. including a gene for plant pigmentation. Frontiers in Plant Science 10: 797. https://doi.org/10.3389/fpls.2019.00797
Fernández-Escobar, J., Rodríguez-Ojeda M.I., Fernández-Martínez J.M., Alonso L.C., 2009. Sunflower broomrape (Orobanche cumana Wallr.) in Castilla-León, a traditionally non-broomrape infested area in Northern Spain. Helia 51: 57–64. https://doi.org/10.2298/HEL0951057F
Fernández-Martínez, J.M., Pérez-Vich B., Akhtouch B., Velasco L., Muñoz-Ruz J., Melero-Vara J.M., Domínguez J., 2004. Registration of four sunflower germplasm lines resistant to race F of broomrape. Crop Science 44: 1033–1034. https://doi.org/10.2135/cropsci2004.1033
Fernández-Martínez J.M., Pérez-Vich B., Velasco L., 2015. Sunflower broomrape (Orobanche cumana Wallr.), in Sunflower Oilseed. Chemistry, Production, Processing and Utilization (E. Martínez-Force, N.T. Dunford, and B.K. Salas, eds.), AOC Press, Champaign, IL (USA), 129–156.
Gagne G., Roeckel-Drevet P., Grezes-Besset B., Shindrova P., Ivanov P., Grand-Ravel C., Vear F., Tourvieille de Labrouhe D., Charmet G., Nicolas P., 1998. Study of the variability and evolution of Orobanche cumana populations infesting sunflower in different European countries. Theoretical and Applied Genetics 96: 1216–1222. https://doi.org/10.1007/s001220050859
González-Cantón, E., Velasco-Sanchez A., Velasco L., Martin-Sanz A., 2019. First report of sunflower broomrape (Orobanche cumana Wallr.) in Portugal. Plant Disease 103: 2143. https://doi.org/10.1094/PDIS-10-18-1723-PDN
Guchetl S.Z., Antonova T.S., Tchelustnikova T.A., 2014. Genetic similarity and differences between the Orobanche cumana Wallr. populations from Russia, Kazakhstan, and Romania revealed using the markers of simple sequence repeat. Russian Agricultural Sciences 40: 326–330. https://doi.org/10.3103/S1068367414050103
Jebri, M., Ben Khalifa M., Fakhfakh H., Pérez-Vich B., Velasco L., 2017. Genetic diversity and race composition of sunflower broomrape populations from Tunisia. Phytopathologia Mediterranea 56, 421–430. https://doi.org/10.14601/Phytopathol_Mediterr-20839
Jestin C., Lecomte V., Duroueix F., 2014. Current situation of sunflower broomrape in France. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 28−31.
Kaya, Y., 2014. Current situation of sunflower broomrape around the world. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 9−18.
Ma D.T., Jan C.C., 2014. Distribution and race composition of sunflower broomrape (Orobanche cumana Wallr.) in Northern China. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 65−69.
Malek J., del Moral L., Fernández-Escobar J., Pérez-Vich B., Velasco L., 2017. Racial characterization and genetic diversity of sunflower broomrape populations from Northern Spain. Phytopathologia Mediterranea 56: 70−76. https://doi.org/10.14601/Phytopathol_Mediterr-19163
Martín-Sanz A., Malek J., Fernández-Martínez J.M., Pérez-Vich B., Velasco L., 2016. Increased virulence in sunflower broomrape (Orobanche cumana Wallr.) populations from Southern Spain is associated with greater genetic diversity. Frontiers in Plant Science 7: 589. https://doi.org/10.3389/fpls.2016.00589.
Melero-Vara, J.M., Domínguez J., Fernández-Martínez J.M., 2000. Update on sunflower broomrape situation in Spain: Racial status and sunflower breeding for resistance. Helia 33: 45−56. https://doi.org/10.1515/helia.2000.23.33.45
Molinero-Ruiz M.L., Domínguez J., 2014. Current situation of sunflower broomrape in Spain. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 19−27.
Molinero-Ruiz M.L., Pérez-Vich B., Pineda-Martos R., Melero-Vara J.M., 2008. Indigenous highly virulent accessions of the sunflower root parasitic weed Orobanche cumana. Weed Research 48: 169–178. https://doi.org/10.1111/j.1365-3180.2007.00611.x
Nabloussi, A., Fernández-Cuesta A., El-Fechtali M., Fernández-Martínez J.M., Velasco L., 2011. Performance and seed quality of Moroccan sunflower varieties and Spanish landraces used for confectionery and snack food. Helia 55: 75–82. https://doi.org/10.2298/HEL1155075N
Nabloussi, A., Velasco L., Assissel N., 2018. First report of sunflower broomrape, Orobanche cumana Wallr, in Morocco. Plant Disease 102, 457. https://doi.org/10.1094/PDIS-06-17-0858-PDN
Parker C., 2016. Orobanche cumana (sunflower broomrape). In: Invasive Species Compendium. Wallingford, UK: CAB International. www.cabi.org/isc.
Pérez-Vich, B., Akhtouch B., Knapp S.J., Leon A.J., Velasco L., Fernández-Martínez J.M., Berry S.T., 2004. Quantitative trait loci analysis of broomrape (Orobanche cumana Wallr.) resistance in sunflower. Theoretical and Applied Genetics 109: 92–102. https://doi.org/10.1007/s00122-004-1599-7
Pineda-Martos R., Velasco L., Fernández-Escobar J., Fernández-Martínez J.M., Pérez-Vich B., 2013. Genetic diversity of sunflower broomrape (Orobanche cumana) populations from Spain. Weed Research 53: 279–289. https://doi.org/10.1111/wre.12022
Pineda-Martos R., Velasco L., Pérez-Vich B., 2014a. Identification, characterisation and discriminatory power of microsatellite markers in the parasitic weed Orobanche cumana. Weed Research 54: 120–132. https://doi.org/10.1111/wre.12062
Pineda-Martos R., Pujadas-Salvà A.J., Fernández-Martínez J.M., Stoyanov K., Velasco L., Pérez-Vich B., 2014b. The genetic structure of wild Orobanche cumana Wallr. (Orobanchaceae) populations in Eastern Bulgaria reflects introgressions from weedy populations. Scientific World Journal 150432. https://doi.org/10.1155/2014/150432
Rodríguez-Ojeda M.I., Fernández-Martínez J.M., Velasco L., Pérez-Vich B., 2013. Extent of cross-fertilization in Orobanche cumana Wallr. Biologia Plantarum 57: 559–562. https://doi.org/10.1007/s10535-012-0301-1
Velasco, L., Pérez-Vich B., Yassein A.A.M., Jan C.C., Fernández-Martínez J.M., 2012. Inheritance of resistance to sunflower broomrape (Orobanche cumana Wallr.) in an interspecific cross between Helianthus annuus and H. debilis subsp. tardiflorus. Plant Breeding 131: 220–221. https://doi.org/10.1111/j.1439-0523.2011.01915.x
Vrânceanu A.V., Tudor V.A., Stoenescu F.M., Pirvu N., 1980. Virulence groups of Orobanche cumana Wallr., differential hosts and resistance source genes in sunflower. In: Proceedings of the 9th International Sunflower Conference, International Sunflower Association, Paris, France, 74–82.
Antonova T., 2014. The history of interconnected evolution of Orobanche cumana Wallr. and sunflower in the Russian Federation and Kazakhstan. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 57−64.
Antonova T.S., Araslanova N.M., Strelnikov E.A., Ramazanova S.A., Guchetl S.Z., Chelyustnikova T.A., 2013. Distribution of highly virulent races of sunflower broomrape (Orobanche cumana Wallr.) in the southern regions of the Russian Federation. Russian Agricultural Sciences 39: 46–50. https://doi.org/10.3103/S1068367413010023
Batchvarova R., 2014. Current situation of sunflower broomrape in Bulgaria. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 51−54.
Beck-Mannagetta G., 1930. Orobanchaceae. In: Das Pflanzenreich Vol. IV (A. Engler, ed.), Verlag von Wilhelm Engelmann, Leipzig, 1−348.
Bilgen B.B., Barut A.K., Demirbaş S., 2019. Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers. Turkish Journal of Botany 43: 3. https://doi.org/10.3906/bot-1807-71
Calderón-González, A., Pouilly N., Muños S., Grand X., Coque M., Velasco L., Pérez-Vich B., 2019. A SSR-SNP linkage map of the parasitic weed Orobanche cumana Wallr. including a gene for plant pigmentation. Frontiers in Plant Science 10: 797. https://doi.org/10.3389/fpls.2019.00797
Fernández-Escobar, J., Rodríguez-Ojeda M.I., Fernández-Martínez J.M., Alonso L.C., 2009. Sunflower broomrape (Orobanche cumana Wallr.) in Castilla-León, a traditionally non-broomrape infested area in Northern Spain. Helia 51: 57–64. https://doi.org/10.2298/HEL0951057F
Fernández-Martínez, J.M., Pérez-Vich B., Akhtouch B., Velasco L., Muñoz-Ruz J., Melero-Vara J.M., Domínguez J., 2004. Registration of four sunflower germplasm lines resistant to race F of broomrape. Crop Science 44: 1033–1034. https://doi.org/10.2135/cropsci2004.1033
Fernández-Martínez J.M., Pérez-Vich B., Velasco L., 2015. Sunflower broomrape (Orobanche cumana Wallr.), in Sunflower Oilseed. Chemistry, Production, Processing and Utilization (E. Martínez-Force, N.T. Dunford, and B.K. Salas, eds.), AOC Press, Champaign, IL (USA), 129–156.
Gagne G., Roeckel-Drevet P., Grezes-Besset B., Shindrova P., Ivanov P., Grand-Ravel C., Vear F., Tourvieille de Labrouhe D., Charmet G., Nicolas P., 1998. Study of the variability and evolution of Orobanche cumana populations infesting sunflower in different European countries. Theoretical and Applied Genetics 96: 1216–1222. https://doi.org/10.1007/s001220050859
González-Cantón, E., Velasco-Sanchez A., Velasco L., Martin-Sanz A., 2019. First report of sunflower broomrape (Orobanche cumana Wallr.) in Portugal. Plant Disease 103: 2143. https://doi.org/10.1094/PDIS-10-18-1723-PDN
Guchetl S.Z., Antonova T.S., Tchelustnikova T.A., 2014. Genetic similarity and differences between the Orobanche cumana Wallr. populations from Russia, Kazakhstan, and Romania revealed using the markers of simple sequence repeat. Russian Agricultural Sciences 40: 326–330. https://doi.org/10.3103/S1068367414050103
Jebri, M., Ben Khalifa M., Fakhfakh H., Pérez-Vich B., Velasco L., 2017. Genetic diversity and race composition of sunflower broomrape populations from Tunisia. Phytopathologia Mediterranea 56, 421–430. https://doi.org/10.14601/Phytopathol_Mediterr-20839
Jestin C., Lecomte V., Duroueix F., 2014. Current situation of sunflower broomrape in France. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 28−31.
Kaya, Y., 2014. Current situation of sunflower broomrape around the world. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 9−18.
Ma D.T., Jan C.C., 2014. Distribution and race composition of sunflower broomrape (Orobanche cumana Wallr.) in Northern China. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 65−69.
Malek J., del Moral L., Fernández-Escobar J., Pérez-Vich B., Velasco L., 2017. Racial characterization and genetic diversity of sunflower broomrape populations from Northern Spain. Phytopathologia Mediterranea 56: 70−76. https://doi.org/10.14601/Phytopathol_Mediterr-19163
Martín-Sanz A., Malek J., Fernández-Martínez J.M., Pérez-Vich B., Velasco L., 2016. Increased virulence in sunflower broomrape (Orobanche cumana Wallr.) populations from Southern Spain is associated with greater genetic diversity. Frontiers in Plant Science 7: 589. https://doi.org/10.3389/fpls.2016.00589.
Melero-Vara, J.M., Domínguez J., Fernández-Martínez J.M., 2000. Update on sunflower broomrape situation in Spain: Racial status and sunflower breeding for resistance. Helia 33: 45−56. https://doi.org/10.1515/helia.2000.23.33.45
Molinero-Ruiz M.L., Domínguez J., 2014. Current situation of sunflower broomrape in Spain. In: Proceedings of the 3rd International Symposium on Broomrape (Orobanche spp.) in Sunflower, International Sunflower Association, Paris, France, 19−27.
Molinero-Ruiz M.L., Pérez-Vich B., Pineda-Martos R., Melero-Vara J.M., 2008. Indigenous highly virulent accessions of the sunflower root parasitic weed Orobanche cumana. Weed Research 48: 169–178. https://doi.org/10.1111/j.1365-3180.2007.00611.x
Nabloussi, A., Fernández-Cuesta A., El-Fechtali M., Fernández-Martínez J.M., Velasco L., 2011. Performance and seed quality of Moroccan sunflower varieties and Spanish landraces used for confectionery and snack food. Helia 55: 75–82. https://doi.org/10.2298/HEL1155075N
Nabloussi, A., Velasco L., Assissel N., 2018. First report of sunflower broomrape, Orobanche cumana Wallr, in Morocco. Plant Disease 102, 457. https://doi.org/10.1094/PDIS-06-17-0858-PDN
Parker C., 2016. Orobanche cumana (sunflower broomrape). In: Invasive Species Compendium. Wallingford, UK: CAB International. www.cabi.org/isc.
Pérez-Vich, B., Akhtouch B., Knapp S.J., Leon A.J., Velasco L., Fernández-Martínez J.M., Berry S.T., 2004. Quantitative trait loci analysis of broomrape (Orobanche cumana Wallr.) resistance in sunflower. Theoretical and Applied Genetics 109: 92–102. https://doi.org/10.1007/s00122-004-1599-7
Pineda-Martos R., Velasco L., Fernández-Escobar J., Fernández-Martínez J.M., Pérez-Vich B., 2013. Genetic diversity of sunflower broomrape (Orobanche cumana) populations from Spain. Weed Research 53: 279–289. https://doi.org/10.1111/wre.12022
Pineda-Martos R., Velasco L., Pérez-Vich B., 2014a. Identification, characterisation and discriminatory power of microsatellite markers in the parasitic weed Orobanche cumana. Weed Research 54: 120–132. https://doi.org/10.1111/wre.12062
Pineda-Martos R., Pujadas-Salvà A.J., Fernández-Martínez J.M., Stoyanov K., Velasco L., Pérez-Vich B., 2014b. The genetic structure of wild Orobanche cumana Wallr. (Orobanchaceae) populations in Eastern Bulgaria reflects introgressions from weedy populations. Scientific World Journal 150432. https://doi.org/10.1155/2014/150432
Rodríguez-Ojeda M.I., Fernández-Martínez J.M., Velasco L., Pérez-Vich B., 2013. Extent of cross-fertilization in Orobanche cumana Wallr. Biologia Plantarum 57: 559–562. https://doi.org/10.1007/s10535-012-0301-1
Velasco, L., Pérez-Vich B., Yassein A.A.M., Jan C.C., Fernández-Martínez J.M., 2012. Inheritance of resistance to sunflower broomrape (Orobanche cumana Wallr.) in an interspecific cross between Helianthus annuus and H. debilis subsp. tardiflorus. Plant Breeding 131: 220–221. https://doi.org/10.1111/j.1439-0523.2011.01915.x
Vrânceanu A.V., Tudor V.A., Stoenescu F.M., Pirvu N., 1980. Virulence groups of Orobanche cumana Wallr., differential hosts and resistance source genes in sunflower. In: Proceedings of the 9th International Sunflower Conference, International Sunflower Association, Paris, France, 74–82.