Articles
Published 2023-01-13
Keywords
- RT-PCR,
- sequencing,
- grapevine viruses,
- vitiviruses
How to Cite
[1]
N. SCHIANCHI, S. FLORE, M. JAGUNIĆ, S. SERRA, V. A. . PROTA, and D. VONČINA, “Identification of grapevine virus G and grapevine virus H in Sardinia, Italy”, Phytopathol. Mediterr., vol. 61, no. 3, pp. 505–511, Jan. 2023.
Abstract
Grapevine virus G (GVG) and grapevine virus H (GVH) (genus Vitivirus) are recently discovered viruses. Analysis of 38 samples from Sardinian grapevine cultivars for the presence of GVG and GVH was carried out using RT-PCR. All samples were also tested for grapevine Pinot gris virus (GPGV) using RT-PCR, and for grapevine leafroll virus -1, -2 and -3, grapevine virus A (GVA) and B (GVB), arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV) and grapevine fleck virus (GFkV) using multiplex RT-PCR. GVG was confirmed in four vines, and GVH was detected in only one sample. In phylogenetic analyses of the coat protein (CP) region, the Sardinian GVG isolates clustered separately from isolates from Croatia and New Zealand. The Sardinian GVH isolate clustered with most sequences from other countries, but with greater affinity to isolates from California (USA) for the CP region, whereas it clustered with isolates from Croatia in the RNA-dependent RNA polymerase (RdRp) region. In addition to GVG and GVH, many samples were coinfected with GVA, viruses from the leafroll complex, and GPGV. This is the first record of GVG and GVH occurring in Italy.
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References
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Adams M.J., Candresse T., Hammond J., Kreuze J.F., Martelli G.P., …Yoshikawa N., 2012. Family—Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses, pp 920-941.
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Candresse T., Theil S., Faure C., Marais A., 2018. Determination of the complete genomic sequence of grapevine virus H, a novel vitivirus infecting grapevine. Archives of Virology, 163(1): 277-280. https://doi.org/10.1007/s00705-017-3587-7
Conti M., Milne R.G., Luisoni E., Boccardo G., 1980. A closterovirus from a stem-pitting-diseased grapevine. Phytopathology, 70(5): 394-399. https://doi.org/10.1007/10.1094/Phyto-70-394
Debat H., Zavallo D., Brisbane R.S., Vončina D., Almeida R.P., ... Asurmendi S., 2019. Grapevine virus L: a novel vitivirus in grapevine. European Journal of Plant Pathology, 155(1): 319-328. https://doi.org/10.1007/s10658-019-01727-w
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Garau R., Prota V.A., Piredda R., Boscia D., Prota U., 1994. On the possible relationship between Kober stem grooving and grapevine virus A. Vitis, 33(3): 161-163.
Hall T.A., 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
Hu R., Dias N.P., Soltani N., Vargas-Asencio J.A., Hensley D., ... Hajimorad M.R., 2021. Cultivated and wild grapevines in Tennessee possess overlapping but distinct virus populations. Plant Disease, 105(10): 2785–2791. https://doi.org/10.1094/PDIS-11-20-2483-SC
Jagunić M., Lazarević B., Nikolić K., Stupić D., Preiner D., Vončina D., 2021. Detection, Transmission, and Characterization of Grapevine Virus H in Croatia. Pathogens, 10(12): 1578. https://doi.org/10.3390/pathogens10121578
Kumar S., Stecher G., Li M., Knyaz C., Tamura K., 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35: 1547-1549. https://doi.org/10.1093/molbev/msy096
La Notte P., Minafra A., Saldarelli P., 1997. A spot-PCR technique for the detection of phloem-limited grapevine viruses. Journal of Virological Methods, 66(1): 103-108. https://doi.org/10.1016/S0166-0934(97)00038-4
Martelli G.P., 2018. Where Grapevine Virology is Heading to. In: ‘Proceedings of the 19th Congress of the International Council for the Study of Virus and Virus-Like Diseases of the Grapevine (ICVG)’, Santiago, Chile, 9-12 April, 2018.
Minafra A., Mawassi M., Goszczynski D., Saldarelli P., 2017. Grapevine Vitiviruses. In: Meng B., Martelli G., Golino D., Fuchs M. (eds) Grapevine Viruses: Molecular Biology, Diagnostics and Management. Springer, Cham. https://doi.org/10.1007/978-3-319-57706-7_11
Panailidou P., Lotos L., Sassalou C.L., Gagiano E., Pietersen G., Maliogka V.I., 2021. First Report of Grapevine Virus H in Grapevine in Greece. Plant Disease, 105(9): 2738. https://doi.org/10.1094/PDIS-01-21-0179-PDN
Read D.A., Thompson G.D., Cordeur N.L., Swanevelder D., Pietersen G., 2022. Genomic characterization of grapevine viruses N and O: novel vitiviruses from South Africa. Archives of Virology 167: 611-614. https://doi.org/10.1007/s00705-021-05333-2
Rosa C., Jimenez J.F., Margaria P., Rowhani A., 2011. Symptomatology and effects of viruses associated with rugose wood complex on growth of four different rootstocks. American Journal of Enology and Viticulture, 62: 207-213. https ://doi.org/10.5344/ajev.2011.10104
Rowhani A., Daubert S., Arnold K., Al Rwahnih M., Klaassen V., Uyemoto J.K., 2018. Synergy between grapevine vitiviruses and grapevine leafroll viruses. European Journal of Plant Pathology, 151: 919-925. https://doi.org/10.1007/s10658-018-1426-7
Saldarelli P., Giampetruzzi A., Morelli M., Malossini U., Pirolo C., … Gualandri V., 2015. Genetic variability of Grapevine Pinot gris virus and its association with grapevine leaf mottling and deformation. Phytopathology, 105(4): 555-563. https://doi.org/10.1094/PHYTO-09-14-0241-R
Sciancalepore A., Pio Ribeiro G., Turturo C., Dell’Orco M., Minafra A., Martelli G.P., 2006. Molecular variability of GVA and GVB coat protein genes in naturally infected grapevine accessions. In: Extended abstracts of the 15th meeting of ICVG, Stellenbosch, pp 81–82.
Vončina D., Almeida R.P.P., 2018. Screening of some Croatian autochthonous grapevine varieties reveals a multitude of viruses, including novel ones. Archives of Virology, 163(8): 2239-2243. https://doi.org/10.1007/s00705-018-3850-6
Adams M.J., Candresse T., Hammond J., Kreuze J.F., Martelli G.P., …Yoshikawa N., 2012. Family—Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses, pp 920-941.
Blouin A.G., Keenan S., Napier K.R., Barrero R.A., MacDiarmid R.M., 2018a. Identification of a novel vitivirus from grapevines in New Zealand. Archives of Virology, 163(1): 281-284. https://doi.org/10.1007/s00705-017-3581-0
Blouin A.G., Chooi, K.M., Warren B., Napier K.R., Barrero R.A., MacDiarmid R.M., 2018b. Grapevine virus I, a putative new vitivirus detected in co-infection with grapevine virus G in New Zealand. Archives of Virology, 163(5): 1371-1374. https://doi.org/10.1007/s00705-018-3738-5
Boscia D., Savino V., Minafra A., Namba S., Elicio V., ... Martelli G.P., 1993. Properties of a filamentous virus isolated from grapevines affected by corky bark. Archives of Virology, 130 (1-2): 109-120. https://doi.org/10.1007/BF01319000
Candresse T., Theil S., Faure C., Marais A., 2018. Determination of the complete genomic sequence of grapevine virus H, a novel vitivirus infecting grapevine. Archives of Virology, 163(1): 277-280. https://doi.org/10.1007/s00705-017-3587-7
Conti M., Milne R.G., Luisoni E., Boccardo G., 1980. A closterovirus from a stem-pitting-diseased grapevine. Phytopathology, 70(5): 394-399. https://doi.org/10.1007/10.1094/Phyto-70-394
Debat H., Zavallo D., Brisbane R.S., Vončina D., Almeida R.P., ... Asurmendi S., 2019. Grapevine virus L: a novel vitivirus in grapevine. European Journal of Plant Pathology, 155(1): 319-328. https://doi.org/10.1007/s10658-019-01727-w
Diaz-Lara A., Brisbane R.S., Aram K., Golino D., Al Rwahnih M., 2019. Detection of new vitiviruses infecting grapevine in California. Archives of Virology, 164(10): 2573-2580. https://doi.org/10.1007/s00705-019-04355-1
Diaz-Lara A., Golino D., Al Rwahnih M., 2018. Genomic characterization of grapevine virus J, a novel virus identified in grapevine. Archives of Virology, 163(7): 1965-1967. https://doi.org/10.1007/s00705-018-3793-y
Faggioli F., Anaclerio F., Angelini E., Antonelli M.G., Bertazzon N., Barba M., 2012. Validation of diagnostic protocols for the detection of grapevine viruses covered by phytosanitary rules. In: ‘Proceedings of the 17th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG)’, Davis, California, USA, October 7-14, 2012. http://hdl.handle.net/10449/23674
Garau R., Prota V.A., Piredda R., Boscia D., Prota U., 1994. On the possible relationship between Kober stem grooving and grapevine virus A. Vitis, 33(3): 161-163.
Hall T.A., 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
Hu R., Dias N.P., Soltani N., Vargas-Asencio J.A., Hensley D., ... Hajimorad M.R., 2021. Cultivated and wild grapevines in Tennessee possess overlapping but distinct virus populations. Plant Disease, 105(10): 2785–2791. https://doi.org/10.1094/PDIS-11-20-2483-SC
Jagunić M., Lazarević B., Nikolić K., Stupić D., Preiner D., Vončina D., 2021. Detection, Transmission, and Characterization of Grapevine Virus H in Croatia. Pathogens, 10(12): 1578. https://doi.org/10.3390/pathogens10121578
Kumar S., Stecher G., Li M., Knyaz C., Tamura K., 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35: 1547-1549. https://doi.org/10.1093/molbev/msy096
La Notte P., Minafra A., Saldarelli P., 1997. A spot-PCR technique for the detection of phloem-limited grapevine viruses. Journal of Virological Methods, 66(1): 103-108. https://doi.org/10.1016/S0166-0934(97)00038-4
Martelli G.P., 2018. Where Grapevine Virology is Heading to. In: ‘Proceedings of the 19th Congress of the International Council for the Study of Virus and Virus-Like Diseases of the Grapevine (ICVG)’, Santiago, Chile, 9-12 April, 2018.
Minafra A., Mawassi M., Goszczynski D., Saldarelli P., 2017. Grapevine Vitiviruses. In: Meng B., Martelli G., Golino D., Fuchs M. (eds) Grapevine Viruses: Molecular Biology, Diagnostics and Management. Springer, Cham. https://doi.org/10.1007/978-3-319-57706-7_11
Panailidou P., Lotos L., Sassalou C.L., Gagiano E., Pietersen G., Maliogka V.I., 2021. First Report of Grapevine Virus H in Grapevine in Greece. Plant Disease, 105(9): 2738. https://doi.org/10.1094/PDIS-01-21-0179-PDN
Read D.A., Thompson G.D., Cordeur N.L., Swanevelder D., Pietersen G., 2022. Genomic characterization of grapevine viruses N and O: novel vitiviruses from South Africa. Archives of Virology 167: 611-614. https://doi.org/10.1007/s00705-021-05333-2
Rosa C., Jimenez J.F., Margaria P., Rowhani A., 2011. Symptomatology and effects of viruses associated with rugose wood complex on growth of four different rootstocks. American Journal of Enology and Viticulture, 62: 207-213. https ://doi.org/10.5344/ajev.2011.10104
Rowhani A., Daubert S., Arnold K., Al Rwahnih M., Klaassen V., Uyemoto J.K., 2018. Synergy between grapevine vitiviruses and grapevine leafroll viruses. European Journal of Plant Pathology, 151: 919-925. https://doi.org/10.1007/s10658-018-1426-7
Saldarelli P., Giampetruzzi A., Morelli M., Malossini U., Pirolo C., … Gualandri V., 2015. Genetic variability of Grapevine Pinot gris virus and its association with grapevine leaf mottling and deformation. Phytopathology, 105(4): 555-563. https://doi.org/10.1094/PHYTO-09-14-0241-R
Sciancalepore A., Pio Ribeiro G., Turturo C., Dell’Orco M., Minafra A., Martelli G.P., 2006. Molecular variability of GVA and GVB coat protein genes in naturally infected grapevine accessions. In: Extended abstracts of the 15th meeting of ICVG, Stellenbosch, pp 81–82.
Vončina D., Almeida R.P.P., 2018. Screening of some Croatian autochthonous grapevine varieties reveals a multitude of viruses, including novel ones. Archives of Virology, 163(8): 2239-2243. https://doi.org/10.1007/s00705-018-3850-6