Vol. 61 No. 3 (2022): including the "60th MPU Anniversary Special Section"
Articles

Identification of grapevine virus G and grapevine virus H in Sardinia, Italy

Nicola SCHIANCHI
Department of Agriculture, Section of Plant Pathology, University of Sassari, Viale Italia 39, 07100 Sassari
Sandro FLORE
Department of Agriculture, Section of Plant Pathology, University of Sassari, Viale Italia 39, 07100 Sassari
Martin JAGUNIĆ
University of Zagreb Faculty of Agriculture, Department of Plant Pathology, Svetošimunska cesta 25, 10000 Zagreb
Salvatorica SERRA
Department of Agriculture, Section of Plant Pathology, University of Sassari, Viale Italia 39, 07100 Sassari
Vanda Assunta PROTA
Department of Agriculture, Section of Plant Pathology, University of Sassari, Viale Italia 39, 07100 Sassari
Darko VONČINA
University of Zagreb Faculty of Agriculture, Department of Plant Pathology, Svetošimunska cesta 25, 10000 Zagreb
Published January 13, 2023
Keywords
  • RT-PCR,
  • sequencing,
  • grapevine viruses,
  • vitiviruses
How to Cite
[1]
N. SCHIANCHI, S. FLORE, M. JAGUNIĆ, S. SERRA, V. A. PROTA, and D. VONČINA, “Identification of grapevine virus G and grapevine virus H in Sardinia, Italy”, Phytopathol. Mediterr., vol. 61, no. 3, pp. 505-511, Jan. 2023.

Abstract

Grapevine virus G (GVG) and grapevine virus H (GVH) (genus Vitivirus) are recently discovered viruses. Analysis of 38 samples from Sardinian grapevine cultivars for the presence of GVG and GVH was carried out using RT-PCR. All samples were also tested for grapevine Pinot gris virus (GPGV) using RT-PCR, and for grapevine leafroll virus -1, -2 and -3, grapevine virus A (GVA) and B (GVB), arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV) and grapevine fleck virus (GFkV) using multiplex RT-PCR. GVG was confirmed in four vines, and GVH was detected in only one sample. In phylogenetic analyses of the coat protein (CP) region, the Sardinian GVG isolates clustered separately from isolates from Croatia and New Zealand. The Sardinian GVH isolate clustered with most sequences from other countries, but with greater affinity to isolates from California (USA) for the CP region, whereas it clustered with isolates from Croatia in the RNA-dependent RNA polymerase (RdRp) region. In addition to GVG and GVH, many samples were coinfected with GVA, viruses from the leafroll complex, and GPGV. This is the first record of GVG and GVH occurring in Italy.

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