Vol. 61 No. 1 (2022)
Articles

Molecular analysis of grapevine Pinot gris virus and its association with grapevine leaf mottling and deformation on ‘Vermentino’ grapevines in Sardinia

Nicola Schianchi
Dipartimento di Agraria, Sezione di Patologia Vegetale ed Entomologia, Università degli Studi di Sassari, Viale Italia 39, 07100 Sassari
Safa Oufensou
Dipartimento di Agraria, Sezione di Patologia Vegetale ed Entomologia, Università degli Studi di Sassari, Viale Italia 39, 07100 Sassari
Gabriele Moro
Bioecopest Srl - Science and Technology Park of Sardinia, Strada Provinciale 55, 07041 Alghero SS
Salvatorica Serra
Dipartimento di Agraria, Sezione di Patologia Vegetale ed Entomologia, Università degli Studi di Sassari, Viale Italia 39, 07100 Sassari
Vanda Assunta Prota
Dipartimento di Agraria, Sezione di Patologia Vegetale ed Entomologia, Università degli Studi di Sassari, Viale Italia 39, 07100 Sassari
Published March 25, 2022
Keywords
  • GLMD,
  • GPGV,
  • symptoms,
  • sequencing
How to Cite
[1]
N. Schianchi, S. Oufensou, G. Moro, S. Serra, and V. A. Prota, “Molecular analysis of grapevine Pinot gris virus and its association with grapevine leaf mottling and deformation on ‘Vermentino’ grapevines in Sardinia”, Phytopathol. Mediterr., vol. 61, no. 1, pp. 3-9, Mar. 2022.

Abstract

In 2017–2018, grapevines of cultivar ‘Vermentino’ infected with grapevine Pinot gris virus (GPGV) in Sardinia, Italy, exhibited leaf symptoms of mosaic, chlorotic mottling, and curling, and stunted shoots. Disease incidence assessed in 2018 was greater (67%, 103 symptomatic plants out of 153 monitored) than in 2017 (26%, 40 of 153 plants). All symptomatic samples tested by RT-PCR were positive for GPGV in both years, while 70% (53 of 76) of the asymptomatic samples in 2017, and 42% (19 of 45) in 2018, were also positive for GPGV. Characterizing six GPGV isolates from ‘Vermentino’ by RT-PCR and sequencing of a genomic fragment covering the movement and coat protein genes showed high conservation at the nucleotide level (98.7% to 100.0%) among Sardinian isolates and isolates for which sequence information is available in GenBank. Phylogenetic analysis indicated that most Sardinian GPGV isolates grouped with other European isolates. This is the first characterization of GPGV in a Sardinian vineyard.

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