Articles
Identification of multi-race Fusarium wilt resistance in chickpea (Cicer arietinum L.) using rapid hydroponic phenotyping
Published 2023-04-06
Keywords
- Chickpea introgression lines,
- marker-assisted backcrossing,
- phenotyping
How to Cite
[1]
J. JORBEN, “Identification of multi-race Fusarium wilt resistance in chickpea (Cicer arietinum L.) using rapid hydroponic phenotyping”, Phytopathol. Mediterr., vol. 62, no. 1, pp. 3–15, Apr. 2023.
Copyright (c) 2023 Jawahar Jorben, Apoorva Rao, Srinivasa Nagappa Chowluru, Sakshi Tomar, Neeraj Kumar, Chellapilla Bharadwaj, Basavanagowda Siddanagowda Patil, Khela Ram Soren
This work is licensed under a Creative Commons Attribution 4.0 International License.
Funding data
-
Indian Council of Agricultural Research
Grant numbers 12-143 (D) -
Indian Council of Agricultural Research
Grant numbers 21-21
Abstract
Fusarium wilt, caused by Fusarium oxysporum f. sp. ciceris (Padwick) Matuo and K. Sato is a major cause for low productivity of chickpea. Presence of multiple pathogenic races makes it difficult for the breeder to screen for Fusarium wilt resistance. Twenty-two chickpea genotypes were grown in Hoagland solution and inoculated with five different F. oxysporum races two isolates of each race), including host and pathogens from the major chickpea growing region of India. The resistant chickpea line “WR 315” showed a “highly resistant” reaction, and the susceptible line “JG 62” showed a “highly susceptible” reaction across all pathogen races and isolates. However, the parent lines “Pusa 372” and “JG 11” showed “susceptible” reactions, while the marker-assisted backcrossing (MABC) lines of “Pusa 372” (IL.11,12,14) and “JG 11” (IL.15,16,17) were superior for assessed characters (lengths of roots and shoots, fresh and dry weights), and were highly resistant to most races. This is the first study to use race specific screening of MABC lines using hydroponic host culture in chickpea.
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References
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Haware, M. P., Nene, Y. L., & Natarajan, M. (1996).The survival of Fusarium oxysporum f. sp. ciceri in the soil in the absence of chickpea. Phytopathologiamediterranea, 9-12.
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Li-sha, Z. E. N. G., Shun, L. Y. U., Cai-xian, D. U., Ling, X. I. A., Jian-kun, Z. H. O. U., Fang, W. A. N. G., ... & Jian-Pin, L. I. U. (2015). Identification of banana resistance to Fusarium wilt by method of hydroponics inoculation. Journal of Southern Agriculture, 46(5).
Maitlo, S. A., Syed, R. N., Khanzada, M. A., Rajput, A. Q., Rajput, N. A., &Lodhi, A. M. (2014). Host-response of chickpea cultivars to Fusariumoxysporum f. sp. ciceris. Pakistan Journal of Phytopathology, 26(2), 193-199.
Mannur, D. M., Babbar, A., Thudi, M., Sabbavarapu, M. M., Roorkiwal, M., Sharanabasappa, B. Y., ...&Chamarthi, S. K. (2019). Super Annigeri 1 and improved JG 74: two Fusarium wilt-resistant introgression lines developed using marker- assisted backcrossing approach in chickpea (Cicer arietinum L.). Molecular breeding, 39(1), 2.doi:10.1007/s11032-018-0908-9.
Merga, B., & Haji, J. (2019). Economic importance of chickpea: Production, value, and world trade. Cogent Food & Agriculture, 5(1),1615718. doi:10.1080/23311932.2019.1615718
Neupane, R. K., Sharma, M., Jha, P., Rao, J. N., Rao, B. V., Gaur, P. M., & Nepal, N. (2007). Evaluation of chickpea genotypes for resistance to Fusarium wilt in Nepal.
Pouralibaba, H. R., Rubiales, D., & Fondevilla, S. (2015). Identification of resistance to Fusarium oxysporum f. sp. lentis in Spanish lentil germplasm. European journal of plant pathology, 143(2), 399-405.
R Core Team (2019). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.https://www.r-project.org/
Roorkiwal, M., Bharadwaj, C., Barmukh, R., Dixit, G. P., Thudi, M., Gaur, P. M., ... & Varshney, R. K. (2020). Integrating genomics for chickpea improvement: achievements and opportunities. Theoretical and Applied Genetics, 133(5), 1703-1720.doi:10.1007/s00122-020-03584-2.
Sharma, K. D., Chen, W., &Muehlbauer, F. J. (2005).Genetics of chickpea resistance to five races of Fusarium wilt and a concise set of race differentials for Fusarium oxysporum f. sp. ciceris. Plant Disease, 89(4), 385-390.doi:10.1094/PD-89-0385.
Sheikh, B. A. (2006). Hydroponics: key to sustain agriculture in water stressed and urban environment. Pak. J. Agric., Agril. Eng., Vet. Sci, 22(2), 53-57.
Sharma, M., Babu, T. K., Gaur, P. M., Ghosh, R., Rameshwar, T., Chaudhary, R. G., ...&Sheikh, B. A. (2006). Hydroponics: key to sustain agriculture in water stressed and urban environment. Pak. J. Agric., Agril. Eng., Vet. Sci, 22(2), 53-57.
Srinivasa N, Tusarkanti Bag, Dikshit H, MurulidharAski (2019). Wilt disease of lentil and its management. Wilt Diseases of Crops, Indian Phytopathological society, New Delhi, India:355-399.
STAR, version 2.0.1 2014.Biometrics and Breeding Informatics, PBGB Division, International Rice Research Institute, Los Baños, Laguna.
Taiyun Wei &ViliamSimko (2017). R package "corrplot": Visualization of Correlation Matrix (Version 0.84).https://github.com/taiyun/corrplot.
Taxak, P. C., Khanna, S. M., Bharadwaj, C., Gaikwad, K., Kaur, S., Chopra, M. & Jain, P. K. (2017). Transcriptomic signature of Fusarium toxin in chickpea unveiling wilt pathogenicity pathways and marker discovery. Physiological and Molecular Plant Pathology, 100, 163e177.
Thompson, H. C., Langhans, R. W., Both, A. J., & Albright, L. D. (1998). Shoot and root temperature effects on lettuce growth in a floating hydroponic system. Journal of the American Society for Horticultural Science, 123(3), 361-364.
Trapero Casas, A., & Jiménez Díaz, R. M. (1985). Fungal wilt and root rot diseases of chickpea in southern Spain.
Varshney, R. K., Song, C., Saxena, R. K., Azam, S., Yu, S., Sharpe, A. G., ...& Millan, T. (2013). Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement. Nature biotechnology, 31(3), 240. doi:10.1038/nbt.2491.
Varshney, R. K., Thudi, M., Nayak, S. N., Gaur, P. M., Kashiwagi, J., Krishnamurthy, L., ...& Rathore, A. (2014). Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.). Theoretical and Applied Genetics, 127(2), 445- 462.doi:10.1007/s00122-013-2230-6.
Yadav, S. S., Kumar, J., Turner, N. C., Berger, J., Redden, R., McNeil, D., ... &Bahl, P. N. (2004). Breeding for improved productivity, multiple resistance and wide adaptation in chickpea (Cicer arietinum L.). Plant Genetic Resources, 2(3), 181. doi:10.1079/PGR200448.
Zheng, S. J., García-Bastidas, F. A., Li, X., Zeng, L., Bai, T., Xu, S., ... & Kema, G. H. (2018). New geographical insights of the latest expansion of Fusarium oxysporum f. sp. cubense tropical race 4 into the greater Mekong subregion. Frontiers in plant science, 9, 457.
Anderson, A. J., & Guerra, D. (1985). Responses of bean to root colonization with Pseudomonas putida in a hydroponic system. Phytopathology, 75(9), 992-995.
Barve, M. P., Haware, M. P., Sainani, M. N., Ranjekar, P. K., & Gupta, V. S. (2001). Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India. Theoretical and Applied Genetics, 102(1), 138– 147.doi: 10.1007/s001220051629.
Batista, R. O., Silva, J. L. O., Nicoli, A., Carneiro, P. C. S., Carneiro, J. E. D. S., Paula Júnior, T. J. D., & Queiroz, M. V. D. (2016). Resistance to Fusarium wilt in common bean. Crop Breeding and Applied Biotechnology, 16(3), 226-233.doi:10.1590/1984-70332016v16n3a34.
Belaidi, Hakima. (2016). Screening of Some Chickpea Germplasm Accessions for Resistance to Two Races of Fusarium oxysporum F. Sp. Ciceris, the Causal of Chickpea Wilt Disease.16. 1758-1763.doi:10.5829/idosi.aejaes.2016.1758.1763.
Caballo C, Castro P, Gil J, Millan T, Rubio J, Die JV (2019) Candidate genes expression profiling during wilting in chickpea caused by Fusarium oxysporum f. sp. ciceris race 5. PLoS ONE 14(10): e0224212. https://doi.org/10.1371/ journal.pone.0224212
Cong, L. L., Sun, Y., Wang, Z., Kang, J. M., Zhang, T. J., Biligetu, B., & Yang, Q. C. (2018).A rapid screening method for evaluating resistance of alfalfa (Medicago sativa L.) to Fusarium root rot. Canadian Journal of Plant Pathology, 40(1), 61-69. doi:10.1080/07060661.2017.1402822.
DAFW, Agricultural Statistics at a Glance 2019, Department of Agriculture, Cooperation & Farmer’s Welfare, Directorate of Economics and Statistics, Ministry of Agriculture and Farmers’ Welfare, Government of India 2019,
Dixit, G. P., Srivastava, A. K., & Singh, N. P. (2019).Marching towards self-sufficiency in chickpea. Current Science, 116(2), 239-242.doi:10.18520/cs/v116/i2/239-242.
Dubey, S. C., Priyanka, K., Singh, V., & Singh, B. (2012). Race Profiling and Molecular Diversity Analysis of Fusarium oxysporum f. sp. ciceris Causing Wilt in Chickpea. Journal of Phytopathology, 160(10), 576-587.doi:10.1111/j.1439- 0434.2012.01954.x
FAOSATAT, (2020). Crops.Retrieved from https://www.fao.org/faostat/ en/#data/QC/visualize
Haware, M. P., & Nene, Y. L. (1982).Races of Fusarium oxysporum f. sp. ciceri. Plant disease, 66(9), 809-810.doi:10.1094/PD-66-809.
Haware, M. P., Nene, Y. L., & Natarajan, M. (1996).The survival of Fusarium oxysporum f. sp. ciceri in the soil in the absence of chickpea. Phytopathologiamediterranea, 9-12.
Hickey, L. T., Hafeez, A. N., Robinson, H., Jackson, S. A., Leal-Bertioli, S. C., Tester, M., ... &Wulff, B. B. (2019). Breeding crops to feed 10 billion. Nature biotechnology, 37(7),744-754.doi:10.1038/s41587-019-0152-9.
Hoagland, D. R., &Arnon, D. I. (1950). The water-culture method for growing plants without soil. Circular.California agricultural experiment station, 347(2nd edit).
Jalali BL and Chand H (1992). Chickpea wilt. In: Plant Diseases of International Importance. Vol. 1. Diseases of Cereals and Pulses. U. S. Singh, A.N. Mukhopadhayay, J. Kumar, and H. S. Chaube, eds. Prentice Hall, Englewood Cliffs, NY, USA. p. 429-444
Jiménez-Díaz, R. M., Castillo, P., del Mar Jiménez-Gasco, M., Landa, B. B., &Navas Cortés, J. A. (2015). Fusarium wilt of chickpeas: Biology, ecology and management. Crop Protection, 73, 16-27.doi.10.1016/j.cropro.2015.02.023.
Jimenez-Diaz, R. M., Trapero-Casas, A., & de La Colina, J. C. (1989).Races of Fusarium oxysporum f. sp. ciceri infecting chickpeas in southern Spain. In Vascular wilt diseases of plants (pp. 515-520). Springer, Berlin, Heidelberg.doi:10.1007/978-3-42-73166-239
Jiménez-Fernández, D., Landa, B. B., Kang, S., Jiménez-Díaz, R. M., &Navas-Cortés, J. A. (2013). Quantitative and microscopic assessment of compatible and incompatible interactions between chickpea cultivars and Fusarium oxysporum f. sp. ciceris races. PLoS One, 8(4), e61360.
Li-sha, Z. E. N. G., Shun, L. Y. U., Cai-xian, D. U., Ling, X. I. A., Jian-kun, Z. H. O. U., Fang, W. A. N. G., ... & Jian-Pin, L. I. U. (2015). Identification of banana resistance to Fusarium wilt by method of hydroponics inoculation. Journal of Southern Agriculture, 46(5).
Maitlo, S. A., Syed, R. N., Khanzada, M. A., Rajput, A. Q., Rajput, N. A., &Lodhi, A. M. (2014). Host-response of chickpea cultivars to Fusariumoxysporum f. sp. ciceris. Pakistan Journal of Phytopathology, 26(2), 193-199.
Mannur, D. M., Babbar, A., Thudi, M., Sabbavarapu, M. M., Roorkiwal, M., Sharanabasappa, B. Y., ...&Chamarthi, S. K. (2019). Super Annigeri 1 and improved JG 74: two Fusarium wilt-resistant introgression lines developed using marker- assisted backcrossing approach in chickpea (Cicer arietinum L.). Molecular breeding, 39(1), 2.doi:10.1007/s11032-018-0908-9.
Merga, B., & Haji, J. (2019). Economic importance of chickpea: Production, value, and world trade. Cogent Food & Agriculture, 5(1),1615718. doi:10.1080/23311932.2019.1615718
Neupane, R. K., Sharma, M., Jha, P., Rao, J. N., Rao, B. V., Gaur, P. M., & Nepal, N. (2007). Evaluation of chickpea genotypes for resistance to Fusarium wilt in Nepal.
Pouralibaba, H. R., Rubiales, D., & Fondevilla, S. (2015). Identification of resistance to Fusarium oxysporum f. sp. lentis in Spanish lentil germplasm. European journal of plant pathology, 143(2), 399-405.
R Core Team (2019). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.https://www.r-project.org/
Roorkiwal, M., Bharadwaj, C., Barmukh, R., Dixit, G. P., Thudi, M., Gaur, P. M., ... & Varshney, R. K. (2020). Integrating genomics for chickpea improvement: achievements and opportunities. Theoretical and Applied Genetics, 133(5), 1703-1720.doi:10.1007/s00122-020-03584-2.
Sharma, K. D., Chen, W., &Muehlbauer, F. J. (2005).Genetics of chickpea resistance to five races of Fusarium wilt and a concise set of race differentials for Fusarium oxysporum f. sp. ciceris. Plant Disease, 89(4), 385-390.doi:10.1094/PD-89-0385.
Sheikh, B. A. (2006). Hydroponics: key to sustain agriculture in water stressed and urban environment. Pak. J. Agric., Agril. Eng., Vet. Sci, 22(2), 53-57.
Sharma, M., Babu, T. K., Gaur, P. M., Ghosh, R., Rameshwar, T., Chaudhary, R. G., ...&Sheikh, B. A. (2006). Hydroponics: key to sustain agriculture in water stressed and urban environment. Pak. J. Agric., Agril. Eng., Vet. Sci, 22(2), 53-57.
Srinivasa N, Tusarkanti Bag, Dikshit H, MurulidharAski (2019). Wilt disease of lentil and its management. Wilt Diseases of Crops, Indian Phytopathological society, New Delhi, India:355-399.
STAR, version 2.0.1 2014.Biometrics and Breeding Informatics, PBGB Division, International Rice Research Institute, Los Baños, Laguna.
Taiyun Wei &ViliamSimko (2017). R package "corrplot": Visualization of Correlation Matrix (Version 0.84).https://github.com/taiyun/corrplot.
Taxak, P. C., Khanna, S. M., Bharadwaj, C., Gaikwad, K., Kaur, S., Chopra, M. & Jain, P. K. (2017). Transcriptomic signature of Fusarium toxin in chickpea unveiling wilt pathogenicity pathways and marker discovery. Physiological and Molecular Plant Pathology, 100, 163e177.
Thompson, H. C., Langhans, R. W., Both, A. J., & Albright, L. D. (1998). Shoot and root temperature effects on lettuce growth in a floating hydroponic system. Journal of the American Society for Horticultural Science, 123(3), 361-364.
Trapero Casas, A., & Jiménez Díaz, R. M. (1985). Fungal wilt and root rot diseases of chickpea in southern Spain.
Varshney, R. K., Song, C., Saxena, R. K., Azam, S., Yu, S., Sharpe, A. G., ...& Millan, T. (2013). Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement. Nature biotechnology, 31(3), 240. doi:10.1038/nbt.2491.
Varshney, R. K., Thudi, M., Nayak, S. N., Gaur, P. M., Kashiwagi, J., Krishnamurthy, L., ...& Rathore, A. (2014). Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.). Theoretical and Applied Genetics, 127(2), 445- 462.doi:10.1007/s00122-013-2230-6.
Yadav, S. S., Kumar, J., Turner, N. C., Berger, J., Redden, R., McNeil, D., ... &Bahl, P. N. (2004). Breeding for improved productivity, multiple resistance and wide adaptation in chickpea (Cicer arietinum L.). Plant Genetic Resources, 2(3), 181. doi:10.1079/PGR200448.
Zheng, S. J., García-Bastidas, F. A., Li, X., Zeng, L., Bai, T., Xu, S., ... & Kema, G. H. (2018). New geographical insights of the latest expansion of Fusarium oxysporum f. sp. cubense tropical race 4 into the greater Mekong subregion. Frontiers in plant science, 9, 457.