Mechanisms of resistance to powdery mildew in cucumber
- Signaling pathway genes,
- Podosphaera xanthii
Copyright (c) 2022 Mumin Ibrahim TEK, Ozer CALIS
This work is licensed under a Creative Commons Attribution 4.0 International License.
Grant numbers FYL-2021-5501
Podosphaera xanthii causes powdery mildew of cucumber, and is associated with significant yield and quality losses. Development of resistant or tolerant varieties is the most effective and eco-friendly strategy for powdery mildew management. An important host resistance mechanism is based on the recognition of conserved resistance genes, resulting in durable resistance. To determine powdery mildew resistance mechanisms in cucumber, total RNAs were isolated from the powdery mildew resistant cultivar Meltem, the tolerant line VT18, and the susceptible local variety Camlica. Expression levels of nine genes in these plants were analysed by Reverse Transcription Polymerase Chain Reaction (RT-PCR). The host reactions were assessed using microscope observations of stained specimens. Serine/threonine (STN7), transcription factor (WRKY22), serine/threonine-protein kinase (D6PKL1), and serine/threonine receptor kinase (NFP) genes were induced, as positive regulators in defence mechanisms against powdery mildew. Polygalacturonase Inhibitor (PGIP) did not express after P. xanthii inoculation of Camlica, resulting in susceptibility. After inoculation, callose synthase (CALLOSE) and cinnamyl alcohol dehydrogenase (CAD) gene expression levels were increased in resistant Meltem, but Hypersensitive Reaction (HR) and ROS formation were only linked in the tolerant VT18. Powdery mildew development was less in Meltem than in VT18, indicating that cell wall thickening and HR play separate roles in resistance to this disease.
Barnes W. C., 1961. Multiple disease resistant cucumbers. Proceedings of the American Society for Horticultural Science 77: 417–423.
Chen Q., Yu G., Wang X., Menh X., Lv C. 2020. Genetics and Resistance Mechanism of the Cucumber (Cucumis sativus L.) Against Powdery Mildew. The Journal of Plant Growth Regulation 020-10075-7.
Cui H., Tsuda K., Parker J.E., 2015. Efector-triggered immunity: from pathogen perception to robust defense. Annual Review of Plant Biology 66: 487–511
FAO (2019). Food and Agriculture Organization Statistics. Production/Yield quantities of Cucumbers and gherkins in Turkey http://www.fao.org/faostat/en/#data/QC/visualize [The link verified date 7 May 2021].
Fugieda K., Akiya R. 1962. Genetic study of powdery mildew resistance and spine color on fruit in cucumber. Japanese Society for Horticultural Science 31: 30–32.
Fukino N., Yoshioka Y., Sugiyama M., Sakata Y., Matsumoto S. 2013. Identification and validation of powdery mildew (Podosphaera xanthii)-resistant loci in recombinant inbred lines of cucumber (Cucumis sativus L.). Molecular Breeding 32: 267–277.
Gao Y. F., Liu J. K., Yang F. M., Zhang G. Y., Wang D., Zhang L., Ou Y. B., Yao Y. A. (2020). The WRKY transcription factor WRKY8 promotes resistance to pathogen infection and mediates drought and salt stress tolerance in Solanum lycopersicum. Physiologia Plantarum 168: 98–117.
He X., Li, Y., Pandey S., Yandell B. S., Pathak M., Weng Y. 2013. QTL mapping of powdery mildew resistance in WI 2757 cucumber (Cucumis sativus L.). TAG. Theoretical and applied genetics. Theoretical and Applied Genetics 126: 2149–2161
Kang L., Li J., Zhao T., Xiao F., Tang X., Thilmony R., He S., Zhou J. M. 2003. Interplay of the Arabidopsis nonhost resistance gene NHO1 with bacterial virulence. Proceedings of the National Academy of Sciences of the United States of America 100: 3519–3524.
Kooistra E. 1968. Powdery mildew resistance in cucumber. Euphytica 17: 236–244.
Kim S. J., Kim M. R., Bedgar D. L., Moinuddin S. G., Cardenas C. L., Davin L. B., Kang C., Lewis N. G. 2004. Functional reclassification of the putative cinnamyl alcohol dehydrogenase multigene family in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 101: 1455–1460.
Li Y., Qiu L., Liu X., Zhang Q., Zhuansun X., Fahima T., Krugman T., Sun Q., Xie C. 2020. Glycerol-Induced Powdery Mildew Resistance in Wheat by Regulating Plant Fatty Acid Metabolism, Plant Hormones Cross-Talk, and Pathogenesis-Related Genes International Journal of Molecular Sciences 21: 673.
Liu L., Yuan X., Cai R., Pan J., He H., Yuan L., Guan Y., Zhu L. 2008. Quantitative trait loci for resistance to powdery mildew in cucumber under seedling spray inoculation and leaf disc infection. Journal of Phytopathology 156: 691–697.
Martiansyah I., Amanah D.M., Putranto R.A., (2018). Semi-quantitative RT-PCR analysis of transcripts encoding protease inhibitor in Hevea brasiliensis Muell. Arg latex. Earth and Environmental Science 183.
Miao L., Qin X., Gao L., Li Q., Li S., He C., Li Y., Yu X. 2019. Selection of reference genes for quantitative real-time PCR analysis in cucumber (Cucumis sativus L.), pumpkin (Cucurbita moschata Duch.) and cucumber-pumpkin grafted plants. Peer Journal Life and Environment 7, e6536.
Naumann M., Somerville, S., Voigt, C. 2013. Differences in early callose deposition during adapted and non-adapted powdery mildew infection of resistant Arabidopsis lines. Plant signaling and Behavior 8(6), e24408.
Nie J., He, H., Peng, J., Yang, X., Bie, B., Zhao, J., Wang, Y., Si, L., Pan, Jungsong, R. and Cai, R. 2015. Identification and Fine Mapping of Pm5.1: A Recessive Gene for Powdery Mildew Resistance in Cucumber (Cucumis sativus L.). Molecular Breeding 35: 1–13.
Rong W., Luo, M., Shan, T., Wei, X., Du, L., Xu, H., Zhang Z. 2016. A Wheat Cinnamyl Alcohol Dehydrogenase TaCAD12 Contributes to Host Resistance to the Sharp Eyespot Disease. Frontiers in Plant Science, 7: 1723.
Sakata Y., Kubo, N., Morishita, M., Kitadani, E., Sugiyama, M., Hirai, M. 2006. QTL analysis of powdery mildew resistance in cucumber. Theoretical and Applied Genetics 112: 243–250.
Thordal-Christensen H., Zhang, Z., Wei, Y. and Collinge, D. B. 1997. Subcellular localization of H2O2 in plants: H2O2 accumulation in papillae and hypersensitive response during the barley powdery mildew interaction. Plant Journal 11: 1187-1194.
Wang Y, Vanden L.K., Wen C., Wehner T.C. Weng Y. 2018. QTL mapping of downy and powdery mildew resistances in PI 197088 cucumber with genotyping by-sequencing in RIL population. Theoretical Applied Genetics 131: 597–611.
Yuceson M , Tek M. I., Calis O. 2020. Determination and identification of powdery mildews on domestic, wild and commercial cucurbits. Mediterranean Agricultural Sciences (In Turkish) 33: 207-214.
Zhang S.P., Liu M.M., Miao H., Zhang S.Q., Yang Y., Xie B.Y., Gu X.F. 2011. QTL mapping of resistance genes to powdery mildew in cucumber (Cucumis sativus L.). Scientia Agricultura Sinica 44: 3584–3593.
Zhang C., Badri Anarjan M., Win K. T., Begum S., Lee S. 2021. QTL-seq analysis of powdery mildew resistance in a Korean cucumber inbred line. Theoretical and Applied Genetics 134: 435–451.
Zitter T.A, Hopkins D.L., Thomas C.E. 1996. Compendium of cucurbits diseases. APS Press, Saint Paul, pp. 24-159.