Articles
Activity of essential oils from Syzygium aromaticum and Rosmarinus officinalis against growth and ochratoxin A production by Aspergillus tubingensis and Aspergillus luchuensis from Moroccan grapes
Adil LAAZIZ- Yousra EL HAMMOUDI
- Souad QJIDAA
- Abdelouahed HAJJAJI
- Hassan HAJJAJ
- Geert HAESAERT
- Amina BOUSETA
Published 2022-07-19
Keywords
- Antifungal effect,
- mycotoxins,
- biological control,
- clove,
- rosemary
How to Cite
[1]
A. LAAZIZ, “Activity of essential oils from Syzygium aromaticum and Rosmarinus officinalis against growth and ochratoxin A production by Aspergillus tubingensis and Aspergillus luchuensis from Moroccan grapes”, Phytopathol. Mediterr., vol. 61, no. 2, pp. 299–310, Jul. 2022.
Abstract
Essential oils have been used since ancient times in traditional medicine and agri-food science to preserve food, and to combat human diseases. Essential oils (EOs) from clove and rosemary, obtained by hydro-distillation, were analyzed by GC/MS and evaluated for their antifungal activity against strains of Aspergillus tubingensis and A. luchuensis and their effect on ochratoxin A (OTA) production by these fungi. The major constituent of clove EO was eugenol (86.4%), and of rosemary EO was eucalyptol (35.9%). Mycelium growth inhibition assays showed that the EOs had dose-depend inhibition effects, which reached 36.6% for rosemary EO, and 100% for clove EO, at EO concentration of 200 μL L-1. These reductions were different for different Aspergillus strains, the essential oil type, and the strain/oil type interaction. Analyses of OTA in the culture medium extracts of Aspergillus strains was carried out using HPLC-FLD, and was confirmed by LC-MS/MS for positive controls of two OTA-producing strains of A. tubingensis. In general, OTA was reduced (from 45 to 100% reduction), except for two strains of A. tubingensis, where OTA production was stimulated.
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