Vol. 61 No. 2 (2022)

Activity of essential oils from Syzygium aromaticum and Rosmarinus officinalis against growth and ochratoxin A production by Aspergillus tubingensis and Aspergillus luchuensis from Moroccan grapes

Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, B.P. 1796-Atlas, 30003, Fez
Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, B.P. 1796-Atlas, 30003, Fez
Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, B.P. 1796-Atlas, 30003, Fez
Abdelouahed HAJJAJI
Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, B.P. 1796-Atlas, 30003, Fez
Laboratory of Plant Biotechnology and Molecular Biology, Faculty of Sciences, Moulay Ismail University, B.P. 11201-Zitoune, Meknes
Department of Crops and Plants, Faculty of Bioscience Engineering, Ghent University, Campus Schoonmeersen Building C, Valentin Vaerwyckweg 1, 9000, Ghent
Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, B.P. 1796-Atlas, 30003, Fez
Published July 19, 2022
  • Antifungal effect,
  • mycotoxins,
  • biological control,
  • clove,
  • rosemary
How to Cite
A. LAAZIZ, “Activity of essential oils from Syzygium aromaticum and Rosmarinus officinalis against growth and ochratoxin A production by Aspergillus tubingensis and Aspergillus luchuensis from Moroccan grapes”, Phytopathol. Mediterr., vol. 61, no. 2, pp. 299-310, Jul. 2022.


Essential oils have been used since ancient times in traditional medicine and agri-food science to preserve food, and to combat human diseases. Essential oils (EOs) from clove and rosemary, obtained by hydro-distillation, were analyzed by GC/MS and evaluated for their antifungal activity against strains of Aspergillus tubingensis and A. luchuensis and their effect on ochratoxin A (OTA) production by these fungi. The major constituent of clove EO was eugenol (86.4%), and of rosemary EO was eucalyptol (35.9%). Mycelium growth inhibition assays showed that the EOs had dose-depend inhibition effects, which reached 36.6% for rosemary EO, and 100% for clove EO, at EO concentration of 200 μL L-1. These reductions were different for different Aspergillus strains, the essential oil type, and the strain/oil type interaction. Analyses of OTA in the culture medium extracts of Aspergillus strains was carried out using HPLC-FLD, and was confirmed by LC-MS/MS for positive controls of two OTA-producing strains of A. tubingensis. In general, OTA was reduced (from 45 to 100% reduction), except for two strains of A. tubingensis, where OTA production was stimulated.


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