Vol. 60 No. 1 (2021)
Articles

Molecular detection and identification of a ‘Candidatus Phytoplasma solani’-related strain associated with pumpkin witches’ broom in Xinjiang, China

Xu WANG
College of Mechanical and Electrical Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China
Chun-Guang WANG
College of Mechanical and Electrical Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China
Xiao-Yan LI
College of Mechanical and Electrical Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China
Zheng-Nan LI
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China
Published May 13, 2021
Keywords
  • Cucurbita moschata,
  • pathogen,
  • phylogeny,
  • RFLP
How to Cite
[1]
X. WANG, C.-G. WANG, X.-Y. LI, and Z.-N. LI, “Molecular detection and identification of a ‘Candidatus Phytoplasma solani’-related strain associated with pumpkin witches’ broom in Xinjiang, China”, Phytopathol. Mediterr., vol. 60, no. 1, pp. 63-68, May 2021.

Funding data

Abstract

Pumpkin plants showing symptoms of witches’ broom (PuWB) were observed in Xinjiang Uyghur autonomous region, China, in September 2018. A phytoplasma was detected in symptomatic plants by PCR amplifying portions of the 16S ribosomal and tuf genes. In addition, the phylogeny based on these genes sequencing indicated that the PuWB strain clusters with ‘Candidatus Phytoplasma solani’ (subgroup 16SrXII-A). Furthermore, based on in silico and in vitro restriction fragment length polymorphism analyses, the PuWB phytoplasma was confirmed as a ‘Ca. P. solani’-related strain. This was the first record of the occurrence of phytoplasma presence in pumpkins in China, and the first record of 16SrXII phytoplasma infecting pumpkins in the world.

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