Vol. 57 No. 2 (2018)
Short Notes

Identification of genes differentially expressed in onion infected with Iris yellow spot virus

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  • Ahmed ABDELKHALEK,
  • Adel ELMORSI,
  • Omar ALSHEHABY,
  • Neeti SANAN-MISHRA,
  • Elsayed HAFEZ
Ahmed ABDELKHALEK
Plant Protection and Bimolecular Diagnosis department, ALCRI, City of Scientific Research and Technological Applications, Alexandria, Egypt
Adel ELMORSI
Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
Omar ALSHEHABY
Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
Neeti SANAN-MISHRA
Plant RNAi Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
Elsayed HAFEZ
Plant Protection and Bimolecular Diagnosis department, ALCRI, City of Scientific Research and Technological Applications, Alexandria, Egypt
DOI: https://doi.org/10.14601/Phytopathol_Mediterr-21877

Published 2018-09-17

Keywords

  • DD-PCR,
  • defence system,
  • IYSV

How to Cite

[1]
A. ABDELKHALEK, A. ELMORSI, O. ALSHEHABY, N. SANAN-MISHRA, and E. HAFEZ, “Identification of genes differentially expressed in onion infected with Iris yellow spot virus”, Phytopathol. Mediterr., vol. 57, no. 2, pp. 334–340, Sep. 2018.

Copyright (c) 2018 Ahmed ABDELKHALEK, Adel ELMORSI, Omar ALSHEHABY, Neeti SANAN-MISHRA, Elsayed HAFEZ

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Iris yellow spot virus (IYSV) causes severe damage and economic losses in onion production. Differential display-PCR was used to study changes in the gene expression of IYSV-infected onion plants. Representative up-regulated and down-regulated genes were selected for further study. Based on sequence analysis, the up-regulated genes were identified as retrotransposon protein, disease resistance-like proteins, chitinase, pathogenesis-related protein, cytochrome oxidase, cytochrome c, pentatricopeptide repeat-containing protein and pectin methylesterase. A DNA-binding transcriptional repressor protein gene was greatly down-regulated. . Most of the identified genes are known to play essential roles in plant defence systems, and are newly identified in onion sequences.

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