Vol. 54 No. 3 (2015)
Research Papers

Characterization of a new partitivirus strain in Verticillium dahliae provides further evidence of the spread of the highly virulent defoliating pathotype through new introductions

Mari Carmen CAÑIZARES
Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”- Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”, 29750 Algarrobo-Costa, Málaga, Spain
Encarnación PÉREZ-ARTÉS
Department of Crop Protection, Instituto de Agricultura Sostenible, IAS-CSIC, Alameda del Obispo s/n. Apdo 4084, 14080 Córdoba, Spain
Nicolás GARCÍA-PEDRAJAS
Department of Computing and Numerical Analysis, C2 Building 3rd Floor, Campus Universitario de Rabanales, 14071 Córdoba, Spain
María GARCÍA-PEDRAJAS
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", CSIC

Published 2015-12-30

Keywords

  • Vascular pathogens,
  • defoliating pathotype,
  • migration,
  • mycoviruses

How to Cite

[1]
M. C. CAÑIZARES, E. PÉREZ-ARTÉS, N. GARCÍA-PEDRAJAS, and M. GARCÍA-PEDRAJAS, “Characterization of a new partitivirus strain in Verticillium dahliae provides further evidence of the spread of the highly virulent defoliating pathotype through new introductions”, Phytopathol. Mediterr., vol. 54, no. 3, pp. 516–523, Dec. 2015.

Abstract

The soilborne pathogen Verticillium dahliae, causal agent of Verticillium wilt, has a worldwide distribution and many hosts of agronomic value. The worldwide spread of a highly virulent defoliating (D) pathotype has greatly increased the threat posed by V. dahliae in olive trees. For effective disease management, it is important to know if the D pathotype is spreading long distances from contaminated material, or if D pathotype isolates may have originated locally from native V. dahliae populations several times. We identified a double-stranded RNA mycovirus in an olive D pathotype isolate from Turkey. Sequencing and phylogenetic analysis clustered the virus with members of the family Partitiviridae. The virus was most similar to a partitivirus previously identified in a V. dahliae isolate from cotton in China (VdPV1), with sequence identities of 94% and 91% at the nucleotide level for RNA1 and RNA2, respectively. The virus therefore corresponded to a strain of the established species, and we designated it VdPV1-ol (VdPV1 from olive). The identification of the same viral species in these two fungal isolates from geographically distant origins provides evidence of their relationships, supporting the hypothesis of long-distance movement of V. dahliae isolates.

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