Vol. 53 No. 1 (2014)
Research Papers

Isolation of non-pathogenic <em>Agrobacterium</em> spp. biovar 1 from agricultural soils in Slovenia

Janja LAMOVŠEK
Agricultural Institute of Slovenia Plant Protection Department Hacquetova ulica 17 SI-1000 Ljubljana
Barbara GERIC STARE
Agricultural Institute of Slovenia Plant Protection Department Hacquetova ulica 17 SI-1000 Ljubljana
Gregor UREK
Agricultural Institute of Slovenia Plant Protection Department Hacquetova ulica 17 SI-1000 Ljubljana
Published April 18, 2014
Keywords
  • crown gall,
  • recA
How to Cite
[1]
J. LAMOVŠEK, B. GERIC STARE, and G. UREK, “Isolation of non-pathogenic <em>Agrobacterium</em&gt; spp. biovar 1 from agricultural soils in Slovenia”, Phytopathol. Mediterr., vol. 53, no. 1, pp. 130-139, Apr. 2014.

Abstract

The disease crown gall, which causes damage on perennial agricultural crops, is economically important in many countries. We therefore explored the presence of Agrobacterium spp. in a variety of agricultural soils where fruit trees are grown. Most samples were collected in four regions of Slovenia where a majority of fruit tree plantations are situated. Crown gall-affected plants were not observed on any of the sampling sites. Members of Agrobacterium spp. biovar 1 were isolated from 63 of the 72 soil samples (88%), but none from forest soil. All isolates of this Agrobacterium species complex were determined to be non-pathogenic by biotests and were assigned to genomic species based on a recA allele sequence analysis. Fourty-three isolates were allocated into genomic species G1 and 26 isolates into G4. Among both genomic species, new alleles of the recA gene were recognized: seven new alleles in G1 (denominated G1-15 to G1-21) and four in G4 (G4-7 to G4-10). Two alleles predominated among the Slovenian strains, recA-G1-15 and recA-G4-2. Different colony morphologies were observed between strains of G1 and G4 on KB medium.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...