Vol. 44 No. 2 (2005)
Short Notes

The Use of pac1 Gene from Schizosaccaromyces pombe to Protect Potato from Potato virus Y (PVY) and Potato X (PVX) Infections

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  • C. Colalongo,
  • N. Contaldo,
  • G. Faccioli,
  • P. Pagan
DOI: https://doi.org/10.14601/Phytopathol_Mediterr-1797

Published 2005-04-01

How to Cite

[1]
C. Colalongo, N. Contaldo, G. Faccioli, and P. Pagan, “The Use of pac1 Gene from Schizosaccaromyces pombe to Protect Potato from Potato virus Y (PVY) and Potato X (PVX) Infections”, Phytopathol. Mediterr., vol. 44, no. 2, pp. 226–232, Apr. 2005.

Copyright (c) 2005 C. Colalongo, N. Contaldo, G. Faccioli, P. Pagan

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

The effect that pac1, a yeast-derived ds-RNA specific RNase gene, has on Potato virus X (PVX) and on Potato virus Y (PVY) infections in potato was investigated. Tuber disks of cv. Desirée were transformed with LB4404 Agrobacterium tumefaciens carrying a modified PBI121 binary vector in which the transgene replaced GUS. Transformed plantlets were tested for possible acquired resistance by inoculation with purified virus suspensions at different concentrations and were ELISA-tested for virus content 40 days later. Plants of two clones had a significantly lower PVX content (66 to 75% that of the control, respectively), when inoculated with a 1 µg ml-1 suspension of the virus. One of these clones showed also a reduced PVY accumulation (41% that of the control), when inoculated with a 10 µg ml-1 suspension of this virus. Results demonstrated the practical utility of the pac1 gene for countering potato virus infections.
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