Vol. 44 No. 1 (2005)
Short Notes

Detection of Potato virus Y in Pepper by ELISA-RT-nested PCR

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  • K. Bougatef,
  • R.T. Marrakchi,
  • H.K. El KHil,
  • M.A. Jarboui,
  • A.B. Ammar Elgaaied
DOI: https://doi.org/10.14601/Phytopathol_Mediterr-1784

Published 2005-04-01

How to Cite

[1]
K. Bougatef, R. Marrakchi, H. El KHil, M. Jarboui, and A. Ammar Elgaaied, “Detection of Potato virus Y in Pepper by ELISA-RT-nested PCR”, Phytopathol. Mediterr., vol. 44, no. 1, pp. 75–79, Apr. 2005.

Copyright (c) 2005 K. Bougatef, R.T. Marrakchi, H.K. El KHil, M.A. Jarboui, A.B. Ammar Elgaaied

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

We describe a reliable method to improve the detection of potato virus Y in pepper (Capsicum annuum L.), based on nested PCR performed after an ELISA-RT-PCR stage. Many problems were overcome by this approach, particularly those due to PCR inhibitory compounds which seem to be abundant in pepper.
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