Genetic diversity among Fusarium oxysporum f.sp. vasinfectum isolates revealed by UP-PCR and AFLP markers

Abstract

Fusarium wilt of cotton is responsible for significant yield losses worldwide. In this study we used UPPCR and AFLP molecular marker techniques to evaluate and study the genetic similarity and phylogeny of 20 Fusarium oxysporum f. sp. vasinfectum isolates. On the basis of comparisons of genetic similarity matrices, AFLP markers were very similar to UP-PCR markers for estimating intra-specific genetic relationships. Dendrograms constructed with both AFLP and UP-PCR revealed that all accessions of F. oxysporum f. sp. vasinfectum isolates were grouped into the same cluster, in good agreement with the pathogenicity tests. The principal difference between the two techniques was that AFLP markers gave better resolution than UP-PCR for discriminating closely related Fusaria.