Vol. 40 No. 2 (2001)
Short Notes

A simple alternative method for the purification of Citrus tristeza virus

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  • Mehmet Asil Yilmaz,
  • Muharrem Arap Kamberoglu,
  • Selma Ozturk
DOI: https://doi.org/10.14601/Phytopathol_Mediterr-1603

Published 2001-08-01

How to Cite

[1]
. . Mehmet Asil Yilmaz, . . Muharrem Arap Kamberoglu, and . . Selma Ozturk, “A simple alternative method for the purification of Citrus tristeza virus”, Phytopathol. Mediterr., vol. 40, no. 2, pp. 176–178, Aug. 2001.
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Abstract

A simple method for the purification of Citrus tristeza virus is described, based on virus extraction from liquid-nitrogen frozen midribs and cortical tissue of infected Mexican lime, precipitation with polyethylene glicol mol. wt 6000 (PEG 6000) column chromatography on Sephacryl S-300-HR columns, and concentration of viruscontaining fractions by PEG 6000, followed by low-speed (17,200 g) centrifugation. Virus preparations were satisfactorily clean, and were successfully used for the production of an antiserum which is now being evaluated.
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