Vol. 65 No. 1 (2026)
Articles

Resistance and virulence patterns in the pea seed-borne mosaic virus (PSbMV) / lentil (Lens culinaris) pathosystem

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  • Joop A.G. VAN LEUR,
  • Jule H. GEORGE,
  • Safaa G. KUMARI
Joop A.G. VAN LEUR
New South Wales Department of Primary Industries and Regional Development, Tamworth Agricultural Institute, Tamworth, Australia
Jule H. GEORGE
New South Wales Department of Primary Industries and Regional Development, Tamworth Agricultural Institute, Tamworth, Australia
Safaa G. KUMARI
International Center for Agricultural Research in the Dry Areas, Terbol, Lebanon
DOI: https://doi.org/10.36253/phyto-17081
Categories

Published 2026-05-14

Keywords

  • Lentil seed-borne PSbMV strains,
  • BYMV,
  • virus resistance,
  • pathogenicity test,
  • pathotypes

How to Cite

[1]
J. A. VAN LEUR, J. H. GEORGE, and S. G. KUMARI, “Resistance and virulence patterns in the pea seed-borne mosaic virus (PSbMV) / lentil (Lens culinaris) pathosystem”, Phytopathol. Mediterr., vol. 65, no. 1, pp. 121–132, May 2026.

Copyright (c) 2026 Joop A.G. VAN LEUR, Jule H. GEORGE, Safaa G. KUMARI

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Pea seed-borne mosaic virus (PSbMV) can reach high levels of seed infection in Australian field peas, resulting in severe crop losses. Endemic, pea-derived PSbMV strains can cause lentil seed infection under experimental conditions, but PSbMV transmission has not been detected in grain harvested from lentil crops in Australia. In contrast, specialised PSbMV strains that are seed-borne in lentils occur in countries with long histories of lentil cultivation. A total of 29 PSbMV isolates were obtained from seeds of 11 exotic lentil accessions held at the Australian Grains Gene-bank, and the isolates were identified as the P2 pathotype using the standard set of pea differentials. However, testing with an additional set of lentil genotypes revealed two distinct pathotypes, tentatively designated P2a and P2b. Screening of lentil accessions previously reported to possess resistance to PSbMV or to bean yellow mosaic virus (BYMV), as well as Australian (25) and North American (3) cultivars, identified resistance to the P2b pathotype in several entries. In contrast, resistance to the more virulent P2a pathotype was only detected in three germplasm accessions that were previously reported to be BYMV resistant. Incursions of lentil seed-borne PSbMV strains pose major risks to the Australian lentil industry. Collaboration with research programmes in countries where lentil seed-borne PSbMV is present will facilitate resistance screening against a possible wider range of pathotypes, and support research on virulence genes and virus genes controlling seed transmission. As large-scale testing with exotic virus strains is difficult to implement in Australia, development of molecular markers for resistance to the most virulent PSbMV strains is desirable.

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References

  1. Abraham A., Makkouk K.M., 2002. The incidence and distribution of seed-transmitted viruses in pea and lentil seed lots in Ethiopia. Seed Science and Technology 30: 567–574.
  2. Ademe A., Kumari S.G., Moukahel A., Alemu T., Abraham A., … Ahmed S., 2025. Phylogenetic analysis, mixed infection and seed transmission of Pea seed-borne mosaic virus in Ethiopia, Physiological and Molecular Plant Pathology 136: 102531. https://doi.org/10.1016/j.pmpp.2024.102531
  3. Agriculture Victoria, 2022. Temperate pulse viruses: pea seed-borne mosaic virus. Accessed March 1, 2026, from https://agriculture.vic.gov.au/
  4. Alconero R., Provvidenti R., Gonsalves D., 1986. Three pea seedborne mosaic virus pathotypes from pea and lentil germ plasm. Plant Disease 70: 783–786. https://doi.org/10.1094/PD-70-783
  5. Alconero R., Hoch J.G., 1989. Incidence of pea seedborne mosaic virus pathotypes in the US national Pisum germplasm collection. Annals of Applied Biology 114: 311–315. https://doi.org/10.1111/j.1744-7348.1989.tb02107.x
  6. Al Khalaf M., Kumari S.G., Haj Kassem A., Makkouk K.M., Al Chaabi A., 2009. Differentiation between susceptible and resistant faba bean, lentil and pea genotypes to Bean yellow mosaic virus on the basis of virus movement and multiplication. Arab Journal of Plant Protection 27: 165–173.
  7. Bayaa B., Kumari S.G., Akkaya A.W., Erskine W., Makkouk K.M., … Özberk, I., 1998. Survey of major bìotìc stresses of lentil in South-East Anatolia, Turkey. Phytopathologia Mediterranea 37: 88–95.
  8. Bekele B., Kumari S.G., Ali K., Yusuf A., Makkouk K.M., … Hailu D., 2005. Survey of viruses affecting legume crops in the Amhara and Oromia Regions of Ethiopia. Phytopathologia Mediterranea 44: 235–246. https://doi.org/10.14601/Phytopathol_Mediterr-1803
  9. Bruun-Rasmussen M., Moller I.S., Tulinius G., Hansen J.K.R., Lund O.S., Johansen I.E., 2007. The same allele of translation initiation factor 4E mediates resistance against two Potyvirus spp. in Pisum sativum. Molecular Plant-Microbe Interactions 20: 1075–1182. https://doi.org/10.1094/MPMI-20-9-1075
  10. Congdon B.S., Coutts B.A., Renton M., Banovic M., Jones R.A.C., 2016. Pea seed-borne mosaic virus in field pea: widespread infection, genetic diversity, and resistance gene effectiveness. Plant Disease 100: 2475–2482. https://doi.org/10.1094/PDIS-05-16-0670-RE
  11. Coutts B.A., Prince R.T., Jones R.A.C., 2008. Further studies on Pea seed-borne mosaic virus in cool-season crop legumes: responses to infection and seed quality defects. Australian Journal of Agricultural Research 59: 1130–1145. https://doi.org/10.1071/AR08113
  12. El Muadhidi M., Makkouk K.M., Kumari S.G., Jerjess M., Murad S.S., … Tarik F., 2001. Survey for legume and cereal viruses in Iraq. Phytopathologia Mediterranea 40: 224–233.
  13. FAOSTAT, 2026. United Nations Food and Agriculture Organization, Statistics Division, Rome, Italy. Accessed April 1, 2026, from https://www.fao.org/faostat/en/#data/QCL
  14. Freeman A., Spackman M., Aftab M., McQueen V., King S., … Rodoni B., 2013. Comparison of Tissue blot immunoassay and high throughput PCR for virus-testing samples from a southeastern Australian pulse virus survey. Australasian Plant Pathology 42: 675–683. https://doi.org/10.1007/s13313-013-0252-9
  15. Freeman A., 2014. DAV00048 - Victorian pulse pathology and virology support program – Final Report. Grains Research and Development Corporation, Canberra, Australia. Accessed December 11, 2025, from https://grdc.com.au/research/reports/report?id=319
  16. Gao Z., Johansen I.E., Eyers S., Thomas C.L., Ellis T.H.N., Maule A.J., 2004. The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking. The Plant Journal 40: 376–385. https://doi.org/10.1111/j.1365-313X.2004.02215.x
  17. Goodell J.J., Hampton R.O., 1984 Ecological characteristics of the lentil strain of pea seedborne mosaic virus. Plant Disease 68: 148–150. https://doi.org/10.1094/PD-68-148
  18. Haddad N.J., Muehlbauer F.J., Hampton R.O., 1978. Inheritance of resistance to Pea seed-borne mosaic virus in lentils. Crop Science 18: 613–615. https://doi.org/10.2135/cropsci1978.0011183X001800040022x
  19. Hampton R.O., 1982. Incidence of the lentil strain of Pea seedborne mosaic virus as a contaminant of Lens culinaris germ plasm. Phytopathology 72: 695–698. https://doi.org/10.1094/Phyto-72-695
  20. Hampton R.O., Muehlbauer F.J., 1977. Seed transmission of the Pea seedborne mosaic virus in lentil. Plant Disease Reporter 61: 235–238.
  21. Johansen I.E., Lund O.S., Hjulsager C.K., Laursen J., 2001. Recessive resistance in Pisum sativum and potyvirus pathotype resolved in a gene-for-cistron correspondence between host and virus. Journal of Virology 75: 6609–6614. https://doi.org/10.1128/JVI.75.14.6609–6614.2001
  22. Jones R.A.C., Congdon B.S., 2024. Australian cool-season pulse seed-borne virus research: 1. Alfalfa and cucumber mosaic viruses and less important viruses. Viruses 16: 144. https://doi.org/10.3390/v16010144
  23. Kanawaty A., Kumari S.G., van Leur J., Hammadi H., 2017. Screening of lentil genotypes for resistance to Bean yellow mosaic virus and effect of mixed infection on the susceptibility of some resistant lentil genotypes. Arab Journal of Plant Protection 35: 171–177. https://doi.org/10.22268/AJPP-035.3.171177
  24. Kasimor K., Bagget, J.R., Hampton, R.O., 1997. Pea cultivar susceptibility and inheritance of resistance to the lentil strain (pathotype P2) of pea seedborne mosaic virus. Journal of the American Society for Horticultural Science 122: 325–328. https://doi.org/10.21273/JASHS.122.3.325
  25. Khetarpal R.K., Maury Y., 1987. Pea seed-borne mosaic virus: a review. Agronomie 7: 215–224. https://doi.org/10.1051/agro:19870401
  26. Khadka R.B., Kumari S., van Leur J.A.G., 2024. Prevalence and incidence of lentil viruses in Nepal. Abstract book of the 15th Australasian Plant Virology Workshop, 29–31 October 2024, Gold Coast, Australia. Accessed January 15, 2026, from https://hdl.handle.net/10568/159867
  27. Knights E. J. 1987. Lentil: A potential winter grain legume crop for temperate Australia. Journal of the Australian Institute of Agricultural Science 53: 271–280.
  28. Kumari S.G., Makkouk K.M., Ismail L.D., 1993. Survey of seed-borne viruses in lentil in Syria and their effects on lentil yield. Arab Journal of Plant Protection 11: 28–32.
  29. Kumari S.G., Makkouk K.M., 1995. Variability among twenty lentil genotypes in seed transmission rates and yield loss induced by pea seed-borne mosaic potyvirus infection. Phytopathologia Mediterranea 34: 129–132.
  30. Kumari S.G., Larsen R., Makkouk K.M., Bashir M., 2009. Virus diseases and their control. In: The lentil: botany, production and uses. (W. Erskine, F. J. Muehlbauer, A. Sarker and B. Sharma, ed.), Cambridge, MA, USA, CABI Press, 306–325. https://doi.org/10.1079/9781845934873.0306
  31. Kumari S.G., Moukahel A., El Miziani I., 2022. Diagnostic tools validated by ICARDA’s Germplasm Health Unit (GHU) for detection of legume seed-borne pests. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon. Accessed January 15, 2026, from https://hdl.handle.net/10568/126879
  32. Latham L.J., Jones R.A.C., 2001a. Alfalfa mosaic and pea seed-borne mosaic viruses in cool season crop, annual pasture, and forage legumes: susceptibility, sensitivity and seed transmission. Australian Journal of Agricultural Research 52: 771–790. https://doi.org/10.1071/AR00165
  33. Latham L.J., Jones R.A.C., 2001b. Incidence of virus infection in experimental plots, commercial crops, and seed stocks of cool season crop legumes. Australian Journal of Agricultural Research 52: 397–413. https://doi.org/10.1071/AR00079
  34. Maina S., Jones R.A.C., 2023. Enhancing biosecurity against virus disease threats to Australian grain crops: current situation and future prospects. Frontiers in Horticulture 2: 1263604. https://doi.org/10.3389/fhort.2023.1263604
  35. Maina S., van Leur J., Ghorbanic A., Jones R.A.C., 2025. Analysis of 40 new isolates of bean yellow mosaic virus reveal important new insights into its phylogeny, evolutionary dynamics and biology. Proceedings of the 16th International Symposium of Plant Virus Epidemiology, São Paulo, Brazil. Accessed January 15, 2026, from https://ispve2025.com/files/ispveprocbook.pdf
  36. Makkouk K.M., Bashir M., Jones R.A.C., Kumari S.G., 2001. Survey for viruses in lentil and chickpea crops in Pakistan. Journal of Plant Diseases and Protection 108: 258–268. https://www.jstor.org/stable/45154856
  37. Makkouk K.M., Kumari S.G., Shahraeen N., Fazlali Y., Farzadfar S., … Reza Mansouri A., 2003. Identification and seasonal variation of viral diseases of chickpea and lentil in Iran. Journal of Plant Diseases and Protection 110: 157–169.
  38. Materne M., Reddy A.A., 2007. Commercial cultivation and profitability. In: Lentil: An Ancient Crop for Modern Times. (S.S. Yadav, D.L. McNeil and P.C. Stevenson, ed.), Dordrecht, The Netherlands, Springer, 173–186.
  39. McKirdy S.J., Jones R.A.C., Latham L.J., Coutts B.A., 2000. Bean yellow mosaic potyvirus infection of alternative annual pasture, forage, and cool season crop legumes: susceptibility, sensitivity, and seed transmission. Australian Journal of Agricultural Research 51: 325–345. https://doi.org/10.1071/AR99110
  40. Montejano-Ramírez V., Valencia-Cantero E., 2024. The importance of lentils: An Overview. Agriculture 14, 103. https://doi.org/10.3390/agriculture14010103
  41. Mustafayev E., Kumari S.G., Attar N., Zeynal A., 2011. Viruses infecting chickpea and lentil crops in Azerbaijan. Australasian Plant Pathology 40: 612–620. https://doi.org/10.1007/s13313-011-0094-2
  42. Provvidenti R., Alconero R. 1988. Inheritance of resistance to a lentil strain of pea seed-borne mosaic virus in Pisum sativum. Journal of Heredity 79:45-47. https://doi.org/10.1093/oxfordjournals.jhered.a110444
  43. Provvidenti R., Hampton R.O., 1991. Chromosomal distribution of genes for resistance to seven potyviruses in Pisum sativum. Pisum Genetics 23: 26–28.
  44. Rashed A., Feng X., Prager S.M., Porter L. D., Knodel J.J., … Eigenbrode S.D., 2018. Vector-borne viruses of pulse crops, with a particular emphasis on North American cropping system. Annals of the Entomological Society of America 111: 205–227. https://doi.org/10.1093/aesa/say014
  45. Simmons H.E., Munkvold G.P., 2014. Seed transmission in the Potyviridae. In: Global perspectives on the health of seeds and plant propagation material. Dordrecht, The Netherlands, Springer, 3–15.
  46. Slinkard A.E., 1988. Indianhead lentil as an annual legume green manure crop for Western Canada. Canadian Journal of Plant Science 68: 838.
  47. Swisher Grimm K.D., Porter L.D., 2020. Development and validation of KASP markers for the identification of pea seedborne mosaic virus Pathotype P1 resistance in Pisum sativum. Plant Disease 104: 1824–1830. https://doi.org/10.1094/PDIS-09-19-1920-RE
  48. Tadesse N., Ali K., Gorfu D., Yusuf A., Abraham A., … Kumari S.G., 1999. Survey for chickpea and lentil viruses in Ethiopia. Phytopathologia Mediterranea 38: 149–158.
  49. Thackwray E.L., Materne M.A., Shunmugam A.S., Henares B.M., Lee R.C., Kamphuis L.G., 2024. The history and pedigree of Australian lentil cultivars. Legume Science 6: p.e70006. https://doi.org/10.1002/leg3.70006
  50. van Leur J.A.G., Freeman A., Aftab M., Spackman M., Redden B., Materne M., 2013a. Identification of seed-borne Pea seed-borne mosaic virus in lentil (Lens culinaris) germplasm and strategies to avoid its introduction in commercial Australian lentil fields. Australasian Plant Disease Notes 8: 75–77. https://doi.org/10.1007/s13314-013-0099-5
  51. van Leur J.A.G., Kumari S.G., Aftab M., Leonforte A., Moore S., 2013b. Virus resistance of Australian pea (Pisum sativum) varieties. New Zealand Journal of Crop and Horticultural Science 41: 86–101. https://doi.org/10.1080/01140671.2013.781039
  52. van Leur J., Aftab M., Freeman A., 2025. Pea seed-borne mosaic virus pathotypes isolated from Australian pea (Pisum sativum) seed. Phytopathologia Mediterranea 64: 71–76. https://doi.org/10.36253/phyto-15934
  53. Wang D., Maule A.J., 1994. A model for seed transmission of a plant virus: Genetic and structural analysis of pea embryo invasion by Pea seed-borne mosaic virus. The Plant Cell 6: 777–787. https://doi.org/10.1105/tpc.6.6.777
  54. Wale M., Jembere, B., Seyoum E., 2000. Biology of the pea aphid, Acyrthosiphon pisum (Harris) (Homoptera: Aphididae) on cool-season legumes. International Journal of Tropical Insect Science 20: 171–180. https://doi.org/10.1017/S1742758400019603
  55. Wilson V.E., Muehlbauer F.J., 1983. Registration notice: ‘Redchief’ lentil. Crop Science 23: 802–803. https://doi.org/10.2135/cropsci1983.0011183X002300040062x
  56. Zohary D, 1972. The wild progenitor and the place of origin of the cultivated lentil: Lens culinaris. Economic Botany 26, 326–332. https://doi.org/10.1007/BF028607

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