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EUPHRESCO III-Special Issue on Plant Health Priorities-RESEARCH PAPERS

New citrus tristeza virus strains (Closterovirus tristezae) detected in the Chlef Valley, Algeria

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  • Abdellah Nabil BENTURQUIA,
  • Malika MEZIANE,
  • Khaled DJELOUAH,
  • Samir ALI-AROUS
Abdellah Nabil BENTURQUIA
Natural Bio-resources Laboratory, Hassiba Benbouali University of Chlef, Algeria – Research Laboratory of Crop Production and Protection, Hassiba Benbouali University of Chle, Algeria
Malika MEZIANE
Research Laboratory of Crop Production and Protection, Hassiba Benbouali University of Chle, Algeria
Khaled DJELOUAH
International Centre for Advanced Mediterranean Agronomic Studies (CIHEAM), Mediterranean Agronomic Institute of Bari, Via Ceglie 9, 70010 Valenzano (Bari), Italy
Samir ALI-AROUS
Research Laboratory of Crop Production and Protection, Hassiba Benbouali University of Chle, Algeria
DOI: https://doi.org/10.36253/phyto-17068
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Published 2026-06-10

Keywords

  • Citrus,
  • New strains,
  • MMMs,
  • Algeria,
  • CTV

How to Cite

[1]
A. N. BENTURQUIA, M. MEZIANE, K. DJELOUAH, and S. ALI-AROUS, “New citrus tristeza virus strains (Closterovirus tristezae) detected in the Chlef Valley, Algeria”, Phytopathol. Mediterr., Jun. 2026.

Copyright (c) 2026 Abdellah Nabil BENTURQUIA, Malika MEZIANE, Khaled DJELOUAH, Samir ALI-AROUS

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

rus production is economically important in the Chlef Valley, Algeria, but faces several challenges from diseases that threaten yields, fruit quality, and long-term sustainability of orchards. During recent decades, several citrus orchards have shown decline and severe symptoms, mostly associated with tristeza and caused by the virulent VT strain reported in 2019. To update current distribution and strains of tristeza pathogens and potential aphid vectors, an additional survey was conducted in the Chlef Valley from 2021 to 2024. Samples collected from different citrus areas were serologically assessed, showing an increase in the infection rates from 3.2% in 2019 to 4.24% in 2024. The molecular genotype characterization using strain specific RT-PCRs confirmed the presence of the pre-existing T30 and VT genotypes, and two new strains, S1 and T3, were also detected. This study has confirmed the widespread dissemination of the virulent VT genotype of CTV, and has detected additional CTV strains. Discovery of new strains and the further spread of the severe VT genotype, combined with detection of the aphid vector Aphis gossypii Glover, poses significant threats to the citrus industry in Algeria. This situation requires proactive measures by the National Phytosanitary Services, including extending surveillance to other citrus Algerian production regions.

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References

  1. Afechtal M., D’onghia A.M., Coccusa G.E.M., Djelouah K., 2018. First report of the Citrus tristeza virus resistance-breaking strain in Morocco. Journal of Plant Pathology 100: 351. https://doi.org/10.1007/s42161-018-0059-1
  2. Albiach-Marti MR (2013) The complex genetics of citrus tristeza virus. In: Current Issues In Molecular Virology - Viral Genetics and Biotechnological Applications (Romanowski V, ed.), InTech pp. 1–26.
  3. Ali-Arous S., 2020. Rôle des Pucerons dans la Propagation du Virus de Tristeza (CTV) Responsable du Dépérissement Rapide des Agrumes dans le Moyen Chlef et Identification des Biotypes Vecteurs des Souches Virulentes. PhD thesis: agricultural production and sustainable agricultural development. Abdelhamid Benbadis University of Mostaganem (Algeria). 82 pp.
  4. Ali Arous S., Guenaoui Y., Drais M.I., Djelouah K., 2019. First detection of a virulent strain of Citrus tristeza virus (Closteroviridae) in a citrus orchard of Chlef Valley (Algeria). Bulletin OEPP/EPPO Bulletin 49 (2): 321–326. https://doi.org/10.1111/epp.12563
  5. Bar-Joseph M., Garnsey S.M., Gonsalves D., Moscovitz M., Purcifull D.E.., Clark M.F., Loebenstein G., 1979. The use of enzyme-linked immunosorbent assay for detection of Citrus tristeza virus. Phytopathology 69(2): 190–194. https://doi.org/10.1094/phyto-69-190
  6. Blackman RL., Eastop VF., 2000. Aphids on the World’s Crops. An Identification and Information Guide. 2nd edition. Vol. 1, John Wiley and Sons, Chichester, 466 pp.
  7. Cambra M., Gorris M.T., Marroquın C., Roman M.P., Olmos A., Navarro L., 2000. Incidence and epidemiology of citrus tristeza virus in the Valencian Community of Spain. Virus Research 71(1-2): 85–95. doi: 10.1016/s0168-1702(00)00190-8
  8. Cook G., van Vuuren S.P., Breytenbach J.H., Burger J.T., Maree H.J., 2016. Expanded strainspecific RT‐PCR assay for differential detection of currently known Citrus tristeza virus strains: A useful screening tool. Journal of Phytopathology 164(10): 847–851. https://doi.org/10.1111/jph.12454
  9. Folimonova S.Y., Sun Y.D., 2022. Citrus Tristeza Virus: From Pathogen to Panacea. Annual Review of Virology 9(1): 417–435. https://doi.org/10.146/annurev-virology-100520-114412
  10. Gottwald T.R., Hughes G., 2000. A new survey method for Citrus tristeza virus disease assessment. In: Proceedings 14th Conference of IOCV (Brazil, 1998), IOCV, Riverside (US), 77–78.
  11. Hilf M.E., Mavrodieva V.A., Garnsey S.M., 2005. Genetic marker analysis of a global collection of isolates of Citrus tristeza virus: characterization and distribution of CTV genotypes and association with symptoms. Phytopathology 95: 909–917. https://doi.org/10.1094/PHYTO-95-0909
  12. Larbi D., Belkahla H., Djelouah K., D’Onghia A.M., 2015. Monitoring citrus tristeza virus (CTV) in Algeria and characterization of local isolates. Acta Horticulturae 1065: 767–771. https://doi.org/10.17660/ActaHortic.2015.1065.96
  13. Licciardello G., Scuderi G., Russo M., Bazzano M., Paradiso G., Bar-Joseph M., Catara, A.F., 2025. Progress in Our Understanding of the Cross-Protection Mechanism of CTV-VT No-SY Isolates Against Homologous SY Isolates. Pathogens 14: 701. https://doi.org/10.3390/pathogens14070701
  14. MADRP, 2023. Statistique Agricole, superficies et productions, SERIE B, 2021. Ministry of agriculture, rural development and fishing of Algeria, 77 pp.
  15. Roy A., Ananthakrishnan G., Hartung J.S., Brlansky R.H., 2010. Development and application of a multiplex reverse-transcription polymerase chain reaction assay for screening a global collection of Citrus tristeza virus isolates. Phytopathology 100(10): 1077–1088. https://doi.org/10.1094/phyto-04-10-0102
  16. Saponari M., Manjunath K., Yokomi R.K., 2008. Quantitative detection of Citrus tristeza virus in citrus and aphids by real-time reverse transcription-PCR (TaqMan). Journal of Virological Methods 147(1): 43–53. https://doi.org/10.1016/j.jviromet.2007.07.026
  17. Sun Y., Yokomi R.K., Folimonova S.Y., 2024. Citrus tristeza virus: a century-long challenge for the world’s citrus industries. Annals of Applied Biology 185 (3): 304–322. https://doi.org/10.1111/aab.12939
  18. Yokomi R., Saponari M., Metheny P., Vidalakis G., 2011. Genetic Differentiation and Biology of Citrus tristeza virus Populations Spreading in California. International Organization of Citrus Virologists Conference Proceedings 1957–2010. 18. 10.5070/C58T2153M4.
  19. Yokomi R.K., Selvara V., Maheshwari Y., Saponari M., Giampetruzzi A., Hajeri S., 2017. Identification and Characterization of Citrus tristeza virus Isolates Breaking Resistance in Trifoliate Orange in California. Phytopathology 107: 901–908. https://doi.org/10.1094/PHYTO-01-17-0007-R
  20. Yokomi R., Selvaraj V., Maheshwari Y., Chiumenti M., Saponari M., Subhas Hajeri., 2018. Molecular and biological characterization of a novel mild strain of citrus tristeza virus in California. Archives of Virology 163: 1795–1804. https://doi.org/10.1007/s00705-018-3799-5.